Occurrence of Pseudocowpox virus associated to Bovine viral diarrhea virus-1, Brazilian Amazon

Alves, Pedro Augusto; Figueiredo, P. O.; Oliveira, Cairo Henrique Sousa de; Barbosa, José Diomedes; Lima, Danillo Henrique S.; Silva, Natália S.; Campos, Karinny Ferreira; Oliveira, Carlos Magno Chaves; Barbosa-Stancioli, Edel Figueiredo; Abrahão, Jônatas Santos; Kroon, Erna Geessien; Trindade, Giliane de Souza

doi:10.1016/j.cimid.2016.09.005

Cited by 12 publications

(10 citation statements)

References 31 publications

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“…A BVDV-2b isolate was also detected in lung samples of a wild boar collected in a commercial slaughterhouse in RS in 2012 (Weber et al, 2016a(Weber et al, , 2016b. Alves et al (2016) detected a BVDV-1a genome in the sera of cattle during an outbreak of vesicular disease by pseudocowpox virus in Pará (PA), northern Brazil. Nucleotide sequencing and phylogeny based on the 5 ′ UTR demonstrated a high similarity with Oregon C24V BVDV strain.…”

Section: Animal Health Research Reviewsmentioning

confidence: 96%

A genetic profile of bovine pestiviruses circulating in Brazil (1998–2018)

Flores

Cargnelutti

Monteiro

et al. 2018

Anim. Health. Res. Rev.

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The pestiviruses bovine viral diarrhea virus 1 (BVDV-1), 2 (BVDV-2), and HoBi-like (HoBiPeV) are endemic among Brazilian cattle, the world's largest commercial bovine herd. In the last two decades (1998–2018) over 300 bovine pestiviruses have been partially or fully sequenced in Brazil, including viruses from different regions, different epidemiological backgrounds, and associated with diverse clinical presentations. Phylogenetic analysis of these viruses demonstrated a predominance of BVDV-1 (54.4%), with subgenotypes −1a (33.9% of total) and −1b (16.3%) being more frequent and subgenotypes −1d, −1e, and −1i at very low frequencies. The overall BVDV-2 frequency was 25.7% but it varied largely by region, reaching up to 48% in Southern states. BVDV-2b was the predominant subgenotype (84.8% of BVDV-2), followed by BVDV-2a (8.86%). HoBiPeV accounted for 19.9% (61/307) of the genotyped viruses and were detected at high frequency in cattle from Northeastern states. These findings demonstrate a unique mix of pestivirus species and subgenotypes, unlike that seen in Europe or North America. The design of effective diagnostic tools, vaccines, and control programs for limiting bovine pestivirus infections in Brazil must take into consideration this unique mix of viruses. This article provides a critical review of two decades of genetic identification of pestiviruses in Brazil.

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Section: Animal Health Research Reviewsmentioning

confidence: 96%

A genetic profile of bovine pestiviruses circulating in Brazil (1998–2018)

Flores

Cargnelutti

Monteiro

et al. 2018

Anim. Health. Res. Rev.

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“…Nas últimas décadas, episódios envolvendo coinfecções em bovinos e em humanos também foram registrados em território nacional. A origem e o significado de coinfecções na patogenia das doenças não são totalmente elucidados e, na atualidade, a frequência das coinfecções no Brasil, é também desconhecida, mas encontra-se sob vigilância (Abrahão et al, 2010;Canal et al, 2012;Campos et al, 2011;Alves et al, 2016;Nascimento et al, 2016;Sant'ana et al, 2013; (Untergasser et al, 2007).…”

Section: Methodsunclassified

Verificação Da Temperatura De Distribuição De Refeições Quentes Ofertadas Em Uma Instituição De Longa Permanência Para Idosos E a Correlação Com O Crescimento Microbiológico

Souza¹,

Pinto²,

Sobrinho³

et al. 2019

Pesquisa Científica E Tecnológica Em Microbiologia

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Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0).O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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“…Reactions with amplification of duplicate samples were considered valid, with melting temperature (Tm) values in accordance with the previously established standard curve. The SYBR Green® PCR Master Mix (Applied Biosystems, USA) was used together with the VGF forward and VGF reverse primers, which were designed to amplify a 82‐bp fragment of the vgf gene (Alves et al., ) at concentrations of 400 μM of each primer in a reaction with a final volume of 10 μl. The thermocycling conditions used were as follows: 95°C—10 min; denaturation: 95°C—15 s; pairing and extension: 60°C—60 s (40 cycles); and dissociation curves: 60–95°C, according to the standard protocol from Applied Biosystems (USA).…”

Section: Methodsmentioning

confidence: 99%

Vaccinia virusdetection in dairy products made with milk from experimentally infected cows

Oliveira

Guedes

Rehfeld

et al. 2017

Transbound Emerg Dis

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Summary Vaccinia virus (VACV) is the agent of bovine vaccinia (BV), an emerging zoonosis that causes exanthematic lesions on the teats of dairy cows and on the hands of milkers. The virus has been detected in the milk of naturally infected cows. The objective of this study was to investigate and quantify VACV DNA as well as the presence of infectious virus particles in samples of cheese curd, cheese whey and pasteurized milk produced using milk from cows experimentally inoculated with VACV‐GP2, a Brazilian isolate of VACV (VACV‐BR). VACV DNA was detected in samples of cheese and pasteurized milk at different time points, even after the resolution of the typical lesions caused by VACV, which occurred after 22 days post‐infection (dpi), on average. Moreover, it was possible to detect infectious viral particles in cheese samples on alternate days until 27 dpi. The presence of both VACV DNA and infectious viral particles in cheese samples throughout the clinical course of BV and even after the disappearance of the typical clinical signs of disease draws attention to the risk associated with consumption of the cheese. Furthermore, VACV‐contaminated milk and cheese may represent an occupational risk to cheesemakers who often manipulate milk and cheese curd without wearing gloves.

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Occurrence of Pseudocowpox virus associated to Bovine viral diarrhea virus-1, Brazilian Amazon

Cited by 12 publications

References 31 publications

A genetic profile of bovine pestiviruses circulating in Brazil (1998–2018)

A genetic profile of bovine pestiviruses circulating in Brazil (1998–2018)

Verificação Da Temperatura De Distribuição De Refeições Quentes Ofertadas Em Uma Instituição De Longa Permanência Para Idosos E a Correlação Com O Crescimento Microbiológico

Vaccinia virusdetection in dairy products made with milk from experimentally infected cows

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