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“…Initially, a variety of mobile phases and stationary phases were tested to obtain the best separation and resolution. Method development was started with a less polar mobile phase (Araujo, Zawadneak, Tavares, Benatto, & Mogor, ); however, no peaks were obtained at acceptable retention time with appreciable resolution. The polarity of the mobile phase was then enhanced by the addition of methanol and 10 m m phosphoric acid.…”
Section: Resultsmentioning
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“…Initially, a variety of mobile phases and stationary phases were tested to obtain the best separation and resolution. Method development was started with a less polar mobile phase (Araujo, Zawadneak, Tavares, Benatto, & Mogor, ); however, no peaks were obtained at acceptable retention time with appreciable resolution. The polarity of the mobile phase was then enhanced by the addition of methanol and 10 m m phosphoric acid.…”
Section: Resultsmentioning
“…The Aphis forbesi Weed, commonly known as the root aphid is native to North America (Blackman and Eastop, 2006), it is an aphid pest affecting strawberry crops (Passos et al, 2000;Cédola and Greco, 2010;Araujo et al, 2013;Bernardi et al, 2013;Zawadneak et al, 2014). A. forbesi is also reported as a vector of the strawberry crinkle virus (Babovic and Krczal, 1976).…”
Section: Introductionmentioning