Occurrence of fastidious Campylobacter spp. in fresh meat and poultry using an adapted cultural protocol

Lynch, Orla; Cagney, Claire; McDowell, D.A.; Duffy, Geraldine

doi:10.1016/j.ijfoodmicro.2011.07.037

Cited by 59 publications

(52 citation statements)

References 89 publications

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“…However, the samples were collected at different production stages. Furthermore, other studies con- cerning the prevalence of Campylobacter at the retail level showed a lower proportion of positive samples than obtained in the present investigations (Prencipe et al 2007;Lynch et al 2011). On the other hand, there are some reports showing a higher rate contamination in similar samples, even over 90% (Rozynek et al 2008;Tang et al 2009;Mackiw et al 2012).…”

Section: Discussioncontrasting

confidence: 45%

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Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Wieczorek¹,

Szewczyk²,

Osek³

2012

Vet. Med.

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ABSTRACT:The study was conducted to investigate the presence of Campylobacter spp. in meat sold to consumers at a retail market in Poland. Antimicrobial resistance and the presence of putative virulence genes of the isolates were also examined. A total of 558 meat samples, including beef (n = 105), pork (n = 85), and poultry (n = 368) were collected over an almost three year study period. It was found that 321 samples, all of them originating from poultry, were contaminated with Campylobacter spp. Most of the obtained isolates were classified as C. coli (189 strains, 58.9%), whereas C. jejuni was identified in 132 (41.1%) samples. All Campylobacter strains were susceptible to gentamicin and all but one C. coli isolate to erythromycin. On the other hand, the highest level of resistance among Campylobacter tested was to ciprofloxacin (91% for C. jejuni and 86.1% for C. coli) and nalidixic acid (89.3% for C. jejuni and 85% for C. coli). Furthermore, resistance to two or more classes of antibiotics was found in the majority (60.9%) of Campylobacter spp. and among them one C. coli strain showed resistance to four different classes of antimicrobials. Identification of virulence genes in the isolated Campylobacter showed that all of them had the flaA and cadF genes. The iam marker was found more often in C. coli strains (88.8%) compared to C. jejuni isolates (53.8%). On the other hand, the virB11 gene was identified only in 4.2% of C. coli and in 6.1% of C. jejuni strains, respectively. Furthermore, the prevalence of the cdtA, cdtB, and cdtC genes among C. jejuni strains was 97.7%, 93.2%, 96.2%, respectively, and was significantly higher than for C. coli regarding the cdtC (66.7%) gene. The obtained results showed that the presence of Campylobacter in retail meat may represent a threat to public health.

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Section: Discussioncontrasting

confidence: 45%

“…These findings are in contrast to data obtained by other authors who in similar samples detected mostly C. jejuni (Sallam 2007;Rozynek et al 2008;Bardon et al 2011;Anonymous 2012a). However, there are also some reports were C. coli was more predominant than C. jejuni in poultry meat (Kurincic et al 2005;Lynch et al 2011;Mackiw et al 2012). …”

Section: Discussionmentioning

confidence: 98%

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Wieczorek¹,

Szewczyk²,

Osek³

2012

Vet. Med.

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“…Campylobacter spp, especially C. jejuni subsp. jejuni, has been linked to extensive intestinal infections, including gastrointestinal infections, myocarditis, hepatitis, meningitis, myelitis, pancreatitis, haemolytic-uraemia syndrome and secondary complications, such as Guillain-Barré syndrome [13].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance profiles of Campylobacter species in the South Africa private health care sector

Shobo

Bester

Baijnath

et al. 2016

J Infect Dev Ctries

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Introduction: There is a dearth of surveillance data on clinical Campylobacter in South Africa, particularly in the private healthcare environment. We investigated the prevalence of resistance to first-line antibiotics used to treat campylobacterioses in clinical Campylobacter isolates from a private pathology laboratory. Methodology: Identification of the Campylobacter specific genes were confirmed by PCR. Minimum inhibitory concentrations were determined using the broth micro-dilution method against macrolides (erythromycin, azithromycin), fluoroquinolones (ciprofloxacin, gatifloxacin) and tetracycline. Results: Seventy-two Campylobacter isolates were identified by PCR, with 54 (75%) being classified as C. jejuni and 18 (25%) as C. coli. Of these, 11 (20.4%) C. jejuni and six (33.3%) C. coli strains were resistant to ciprofloxacin and three (7.41%) C. jejuni and three (16.7%) C. coli strains were resistant to gatifloxacin. The number of C. jejuni strains resistant to erythromycin and azithromycin was 17 (31.5%) and 36 (50%) respectively, while the resistance of C. coli strains to erythromycin and azithromycin were seven (38.9%) and 14 (77.8%) respectively. Resistance to tetracycline was detected in 10 (55.6%) C. coli and 14 (25.9%) C. jejuni strains. Conclusion: In the light of these resistant profiles, the lack of a South African Campylobacter surveillance program is of concern. Relatively high prevalence of resistance in clinical isolates of C. jejuni and C. coli to the fluoroquinolones, macrolides and tetracycline used in first line treatment is of great concern. The efficacy treating human campylobacteriosis should thus be revisited.

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“…Given recent studies demonstrating C. concisus is present in well established vectors of disease, namely domestic pets (Petersen et al, 2007) and poultry and beef samples (Lynch et al, 2011), further studies of this organism are certainly warranted.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of Campylobacter concisus strains from South Africa using Amplified Fragment Length Polymorphism (AFLP) profiling and a Genomospecies-specific Polymerase Chain Reaction (PCR) assay: Identification of novel genomospecies and correlation with clinical data

S¹,

B²,

S³

et al. 2013

Afr. J. Microbiol. Res.

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Amplified Fragment Length Polymorphism (AFLP) profiling was used to evaluate the distribution of phenotypically indistinguishable, but genetically distinct, among Campylobacter concisus strains from South Africa. A Polymerase Chain Reaction (PCR) assay described for identifying strains belonging to Genomospecies 1 and 2 was applied in this study. Forty-seven C. concisus strains were studied in total, of which 42 were of South African origin. Forty of the South African isolates were assigned to the major existing genomospecies typified by the type strain of oral origin (GS1), and reference strains from bloody diarrhoea (GS2). Eighteen South African isolates were distributed in the GS1 cluster including two oral strains. Twenty-two faecal South African isolates clustered with reference GS2 strains. Two novel genomospecies (GS 5 and 6) were inferred by their AFLP profile characteristics. Use of an existing PCR assay first described for identification of GS1 and GS2 strains generally indicated that the tool was accurate, although the novel genomospecies described here yielded an amplicon in the GS2 assay. No consistent clinical pattern among the diarrhoea South African strains could be discerned. The study extends the known genetic diversity among C. concisus, elucidates the presence of multiple genomospecies in South Africa, and confirms for the first time an association of GS1 with diarrhoea as well as the utility (with caveats) of a PCR assay for identifying GS1 and GS2 strains.

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Occurrence of fastidious Campylobacter spp. in fresh meat and poultry using an adapted cultural protocol

Cited by 59 publications

References 89 publications

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

Antibiotic resistance profiles of Campylobacter species in the South Africa private health care sector

Characterisation of Campylobacter concisus strains from South Africa using Amplified Fragment Length Polymorphism (AFLP) profiling and a Genomospecies-specific Polymerase Chain Reaction (PCR) assay: Identification of novel genomospecies and correlation with clinical data

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