Occurrence of a Specific Foetal Protein in a Primary Liver Carcinoma

Kithier, Karel; Houštěk, J; Masopust, J; Rádl, J.

doi:10.1038/212414a0

Cited by 41 publications

(7 citation statements)

References 2 publications

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“…However, only 2 out of 19 patients had levels clearly above the normal range. There was no correlation between the serum levels of TBG and alphafetoprotein, a fetal protein commonly found in the serum of patients with primary liver carcinoma (12). It appears, therfore, that elevated serum TBG is not a consistent finding in patients with hepatocellular carcinoma, and that TBG is not a "tumor marker" for this neoplasm, as suggested in a previous study (4).…”

Section: Discussionmentioning

confidence: 42%

Radioimmunoassay for Serum Thyroxine-Binding Globulin: Results in Normal Subjects and in Patients with Hepatocellular Carcinoma

Larsen

Robbins

1976

The Journal of Clinical Endocrinology & Metabolism

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Serum thyroxine-binding globulin (TBG) was measured by radioimmunoassay. The human TBG used in this study was purified by affinity, anion-exchange, and gel filtration chromatography. The serum TBG concentration in 98 euthyroid normals was 1.48 +/- 0.46 mg/100 ml (mean +/- SD), which is one-half that previously reported using a similar method. The level in females (1.66 +/- 0.56) was significantly higher than that in males (1.37 +/- 0.37). Comparison of the serum TBG level and the maximum binding capacity of serum TBG for thyroxine (T4) yielded a molar ratio of 1:1 for T4 and TBG. The mean serum TBG in 19 patients with hepatocellular carcinoma was 2.10 +/- 1.29 mg/100 ml; however, only 2 of these patients had serum TBG levels outside the normal range.

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Section: Discussionmentioning

confidence: 42%

Radioimmunoassay for Serum Thyroxine-Binding Globulin: Results in Normal Subjects and in Patients with Hepatocellular Carcinoma

Larsen

Robbins

1976

The Journal of Clinical Endocrinology & Metabolism

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“…This, and similar observations in mice (Abelev et al, 1963), prompted a whole series of systematic studies on the incidence of AFP in patients with hepatocellular carcinoma and other diseases of the liver (Abelev, 1965;Kithier et al, 1966;Uriel et al, 1967;Abelev, 1968;Alpert et al, 1968;Masopust et al, 1968;Masseyeff et al, 1968;Purves et al, 1968;Uriel et al, 1968;Alpert, 1969;Alpert et al, 1969;Endo et al, 1969;Foli et al, 1969;Hull et al, 1969b;de Necheaud et al, 1969;Economopoulas et al, 1970;Foy et al, 1970b;Hull et al, 1970;O'Conor et al, 1970;Purves et al, 1970a;Abelev, 1971;Kresno et al, 1971;Masseyeff, 1972;Purves et al, 1973b).…”

Section: Afp In Amniotic Fluidmentioning

confidence: 74%

Alpha-feto-protein during development and in disease.

Adinolfi¹,

Adinolfí²,

Lessof³

1975

Journal of Medical Genetics

107

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“…Thus, for example, the cross-reaction of the D14 cell sap antigen, which was also detected in two out of thirteen other hepatomas, with an embryonic serum component compares with the finding of an embryonic serum a globulin in o-aminoazotolueneinduced mouse hepatomas (Abelev et al, 1963). Also an embryonic a globulin has been detected in the serum of a patient with carcinoma of liver (Kithier, Houstek Masopust and Radl, 1966). The finding that several of the antigens in DMABinduced hepatomas, whilst absent from liver, could be found in other normal tissues compares with the observation by Day (1965) that antisera against microsome fractions of 2-acetylaminofluorene-induced rat hepatomas could be shown by fluorescence microscopy to cross-react with rat kidney.…”

Section: Discussionmentioning

confidence: 89%

Antigenic composition of transplanted rat hepatomas originally induced by 4-dimethylaminoazobenzene.

Baldwin¹,

Barker²

1967

Br J Cancer

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ABNORMAL antigens have been demonstrated by immunodiffusion methods in both the soluble cytoplasmic protein (cell sap) and microsome fractions of primary, 4-dimethylaminoazobenzene (DMAB)-induced rat hepatomas which could not be detected in normal or carcinogen treated liver (Baldwin, 1965). Comparable with these observations are the findings of Abelev and Avenirova (1960) showing the presence of abnormal antigens in o-aminoazotoluene-induced mouse hepatomas. Similarly, Hirai et al. (1963) demonstrated new antigens in a soluble protein fraction from a transplanted rat hepatoma originally induced with DMAB and Deckers (1964) identified abnormal microsomal antigens.Further, unequivocal evidence of tumour-specific antigens in DMAB-induced rat hepatomas has been provided by the demonstration that resistance can be evoked against tumour transplanted into isogeneic hosts (Baldwin and Barker, 1965). These studies also indicated that each tumour possessed an individual antigenic specificity since induction of resistance to one tumour did not modify the growth of other DMAB-induced hepatomas.In the previous study (Baldwin, 1965) heterologous antisera were produced against sub-cellular fractions from pools of several primary hepatomas thereby excluding the possibility of examining the specific antigenicities of individual tumours. Accordingly, a more detailed study of the abnormal antigens demonstrable by immunodiffusion methods in individual DMAB-induced hepatomas has been undertaken. For this purpose, the antigenic composition of sub-cellular fractions of two transplanted hepatomas (D8 and D14) have been compared with each other and with a range of other transplanted hepatomas. Furthermore, the tumour specificity of the abnormal antigens demonstrable in these two tumours has been more closely investigated by comparison of the cross-reactions with subcellular fractions of a number of normal tissues and foetal rat serum. MATERIALS AND METHODSRats.-Rats of an inbred Wistar strain were used for all tests. These were maintained on a standard cubed diet (MRC41B) or a low protein diet containing carcinogen with water ad libitum. Induction of TumoursHepatomas were induced by feeding 4-dimethylaminoazobenzene (DMAB) at a level of 0*06 per cent in an unpolished rice-carrot diet for 3 months followed by continuous feeding on the basal diet (Baldwin, 1964). One tumour, a hepatocellular carcinoma (D8), selected for study arose in the liver of a male rat 71

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Occurrence of a Specific Foetal Protein in a Primary Liver Carcinoma

Cited by 41 publications

References 2 publications

Radioimmunoassay for Serum Thyroxine-Binding Globulin: Results in Normal Subjects and in Patients with Hepatocellular Carcinoma

Radioimmunoassay for Serum Thyroxine-Binding Globulin: Results in Normal Subjects and in Patients with Hepatocellular Carcinoma

Alpha-feto-protein during development and in disease.

Antigenic composition of transplanted rat hepatomas originally induced by 4-dimethylaminoazobenzene.

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