Occurrence and Characterization of Aeromonas Species in Pasteurized Milk and White Cheese in Rio De Janeiro, Brazil

Freitas, A.C.; Nunes, Marly Paiva; Milhomem, Arlete Moreira; Ricciardi, Ilvan Delgado

doi:10.4315/0362-028x-56.1.62

Cited by 33 publications

(25 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A presença de A. hydrophila, na cadeia de leite e seus derivados, vem sendo documentada (CARNEIRO; ROSSI Jr., 2006;FREITAS et al, 1993). Bulhões e Rossi Junior (2002), ao estudar a prevalência deste microrganismo no queijo-de-minas frescal artesanal, detectaram a sua presença em 51,2% amostras (82/160), em quantidades que variaram entre 5,0 × 10 3 e 4,0 × 10 5 UFC.g -1 .…”

Section: A Quantificação De Células Planctônicas No Leiteunclassified

Formação de biofilme em aço inoxidável por Aeromonas hydrophila e Staphylococcus aureus usando leite e diferentes condições de cultivo

Boari¹,

Alves²,

Tebaldi³

et al. 2009

Ciênc. Tecnol. Aliment.

View full text Add to dashboard Cite

Biofilmes correspondem a comunidades constituídas por células sésseis, mono ou multiespécies, aderidas a um substrato, embebidas em uma matriz de polímeros extracelulares (exopolissacarídeos -EPS), em cuja formação os microrganismos exibem diferenciados fenótipos, metabolismo, fisiologia e transcrição genética. (DONLAN;COSTERTON, 2002). Como etapas importantes para a sua formação, são descritas as adesões iniciais, passando os microrganismos de seu estilo de vida planctônico ao séssil, à formação de microlônias, à maturação e ao destacamento de células, retornando estas a seu estilo de vida planctônico (Figura 1 e Figura 2).Estas estruturas naturalmente ocorrem em variados tipos de ambientes, sejam eles bióticos ou abióticos. Entretanto, afora a sua ocorrência natural, pesquisas sobre a sua formação em superfícies utilizadas na produção de alimentos, como aço inoxidável American Iron and Steel Institute -AISI 304 (2008), vêm recebendo destaque, principalmente no que se refere aos malefícios de sua presença. Uma vez constituídos, os biofilmes agem como pontos de contaminação constante, liberando células de microrganismos patogênicos e/ou deterioradores, como Staphylococcus aureus e Aeromonas hydrophila, podendo comprometer, assim, a qualidade microbiológica de matérias-primas, produtos pré-acabados e acabados (FUSTER-VALLS et al., 2008). As negatividades de sua ocorrência também se relacionam à corrosão de equipamentos, usualmente designada pela expressão Corrosão Microbiologicamente Influenciada (MIC), e pela redução da capacidade da troca de calor entre superfícies (MANSFELD, 2007). Seguindo à hipótese de que na natureza a constituição destas comunidades oferecia proteção aos indivíduos, o mesmo AbstractThe aim of this research was to verify the capability of biofilm formation on stainless steel by Aeromonas hydrophila and Staphylococcus aureus using milk and different conditions of cultivation. The variables consisted in mono and multi-species cultivation of these microorganisms and in the temperatures of 4, 7 and 18 °C. Containers containing 1000 mL of milk, population density of 10 5 CFU.mL -1 of each microorganism, and ten suspended chips of stainless steel AISI 304 (10 × 20 mm) were used to seal up and storage, under 60 rpm of agitation for 10 days. The analyses were conducted every 48 hours. Sessile cells of A. hydrophila and S. aureus and were enumerated by plating in m-Aeromonas selective agar and Baird-Parker agar. Complementary studies included the generation time calculation, enumeration of planktonic cells, and visualization of chips by scanning electron microscopy. S. aureus, in mono-cultivation, formed biofilm at 18 °C and at 7 °C. At 4 °C was observed attachment. The presence of A. hydrophila reduced the performance of S. aureus. In this condition, S. aureus formed biofilm at 18 °C. A. hydrophila formed biofilm under all conditions. Keywords: Aeromonas hydrophila; Staphylococcus aureus; biofilm; milk; stainless steel; temperature. ResumoO objetivo desta pesquisa consistiu em avaliar a formação de...

show abstract

Section: A Quantificação De Células Planctônicas No Leiteunclassified

Formação de biofilme em aço inoxidável por Aeromonas hydrophila e Staphylococcus aureus usando leite e diferentes condições de cultivo

Boari¹,

Alves²,

Tebaldi³

et al. 2009

Ciênc. Tecnol. Aliment.

View full text Add to dashboard Cite

show abstract

“…This soft, white cheese is made of pasteurized or raw milk. It is characterized by a high water content (43.0%) and low pH (5.1-5.6) (Freitas et al, 1993). Microbiological standards for soft cheese inspection are determined by the Brazilian National Public Health Agency (Portaria…, 1997).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of toxigenic Escherichia coli in raw milk cheese in Brazil

Paneto

Schocken-Iturrino

Macedo

et al. 2007

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

The occurrence of toxigenic Escherichia coli in raw milk cheese was surveyed in Middle Western Brazil. Fifty samples of cheese from different supermarkets were analyzed for E.coli. The isolates were serotyped and screened for the presence of verotoxigenic E. coli (VTEC) and enterotoxigenic E. coli (ETEC) by Polymerase Chain Reaction (PCR). The susceptibility to thirteen antimicrobial agents was evaluated by the disk diffusion method. E.coli were recovered from 48 (96.0%) of the samples. The serogroups identified were O125 (6.0%), O111 (4.0%), O55 (2.0%) and O119 (2.0%). Three (6.0%) and 1(2.0%) of the E.coli isolates were VTEC and ETEC, respectively. Most frequent resistance was observed to the following antimicrobials: cephalothin (60.0%), nalidixic acid (40.0%), doxycyclin (33.0%), tetracycline (31.0%) and ampicillin (29.0%). Keywords: Escherichia coli, cheese, VTEC, ETEC, antimicrobial resistance RESUMO Pesquisou-se a ocorrência de

show abstract

“…Aeromonas genus is considered an emerging pathogen (26,27) and responsible for gastrointestinal disturbances (19), septicemia, endocarditis, conjunctivitis and wound infections (8,9,13,16,18). These organisms are largely distributed in several water sources such as rivers, lakes, wells, sewage, wastewaters and drinking water supplies (19,24).…”

Section: Introductionmentioning

confidence: 99%

Aeromonas presence in drinking water from collective reservoirs and wells in peri-urban area in Brazil

Razzolini¹,

Günther²,

Martone-Rocha³

et al. 2010

Braz. J. Microbiol.

View full text Add to dashboard Cite

Aeromonas genus is considered an emerging pathogen and its presence in drinking water supplies is a reason to public health concern. This study investigated the occurrence of Aeromonas in samples from collective reservoirs and wells used as drinking water sources in a peri-urban area. A total of 35 water samples were collected from collective reservoirs and 32 from wells bimonthly, from September 2007 to September 2008. Aeromonas spp determination was carried out using a Multiple-Tube Technique. Samples were inoculated into alkaline peptone water and the superficial film formed was transferred to blood agar plates amended with ampicillin. Typical Aeromonas colonies were submitted to a biochemical screening and then to biochemical tests for species differentiation. Aeromonas was detected in 13 (19%) of the 69 samples examined (6 from collective reservoirs and 7 from wells). Concentrations of Aeromonas in collective reservoirs ranged from <0.3 to 1.2 x10 2 MPN/100mL and, in wells, from <0.3 to 2.4 x10 2 MPN/100mL. The most frequent specie in the collective reservoir samples was Aeromonas spp (68%), followed by A. encheleia (14%) and A. allosaccharophila (8%) and A. hydrophila (8%). Aeromonas spp (87%) was the most frequent specie isolated from well samples, followed by A. allosacchariphila (8%), A. encheleia (2%) and A. jandaei (5%). These data show the presence and diversity of Aeromonas genus in the samples analyzed and highlight that its presence in drinking water poses a significant public health concern.

show abstract

Occurrence and Characterization of Aeromonas Species in Pasteurized Milk and White Cheese in Rio De Janeiro, Brazil

Cited by 33 publications

References 0 publications

Formação de biofilme em aço inoxidável por Aeromonas hydrophila e Staphylococcus aureus usando leite e diferentes condições de cultivo

Formação de biofilme em aço inoxidável por Aeromonas hydrophila e Staphylococcus aureus usando leite e diferentes condições de cultivo

Occurrence of toxigenic Escherichia coli in raw milk cheese in Brazil

Aeromonas presence in drinking water from collective reservoirs and wells in peri-urban area in Brazil

Contact Info

Product

Resources

About