Occasional transsynaptic viral labeling in the central nervous system from the polycystic ovary induced by estradiol valerate

Abstract

Increased density of catecholaminergic nerves in the human polycystic ovary has been observed. The aim of the present study was to investigate the distribution of transsynaptically virus-labeled neurons in the central nervous system from the rat polycystic ovary to see whether is it different or not from that of cycling control rats. To induce a polycystic ovary, a single injection of estradiol valerate was given to adult female rats and 30 days later a neurotropic virus was injected into the right ovary. Rats were sacrificed 72 or 96 hours after viral infection. Weight of the ovaries of the estradiol valerate-treated rats was significantly lower compared to controls, and the histology of the ovaries of the treated rats displayed severely atretic large antral follicles. There was almost no viral labeling in the central nervous system from the ovaries showing precystic morphology, in spite of the fact that such altered organs are rich in nerve fibres. It is assumed that presently unidentified factors in the precystic ovary, presumably related to the link between the immune and the nervous system, might be involved in the infectivity of the virus, and thus be responsible for the lack of viral labeling from such an ovary.

Effects of metformin on the reproductive system of androgenized female rats

“…Our data on androgenized female rats are consistent with those of previous studies (1)(2)(3)(4)(5)(11)(12). Our findings suggest the animals had altered ovulation and even an absence of ovulation.…”

Neonatal Exposure to Estradiol Valerate Programs Ovarian Sympathetic Innervation and Follicular Development in the Adult Rat1

“…It is interesting to note that the effect of EV was not found at the celiac ganglion. Probably, the estradiol effect is preferentially localized to the ovarian nerves and does not involve the central sympathetic control, as demonstrated previously [30]. Ovarian NGFB concentration in adult rats was lower than that in controls.…”

“…Viral transneuronal tracing studies [30] have demonstrated that EV effect does not involve sympathetic neurons projecting from the hypothalamic paraventricular nucleus, thus supporting a local effect of estradiol on the expression of intraovarian neurotrophic factors. The rapid effect of EV on Ngfb and Ngfr mRNA expression found in our study could be the result of the estrogen action on the estrogen response elements present in the DNA for Ngfb and Ngfr [31].…”

Neonatal exposure to single doses of estradiol or testosterone programs ovarian follicular development–modified hypothalamic neurotransmitters and causes polycystic ovary during adulthood in the rat

“…Indeed, neonatal estradiol-induced increases in glutamate neurotransmission have been clearly demonstrated to occur during puberty (32). The administration of estradiol to adult rats using the same protocol used in the present study produced a deleterious effect on noradrenergic neurotransmission (34,35) and a neurodegenerative effect on opioid neurons in the hypothalamus (36). Therefore, we are tempted to suggest that the administration of EV before the maturation of the reproductive hypothalamus produced a permanent excitatory effect that altered its functioning up to adulthood.…”