Obtenção e caracterização de anticorpo monoclonal murino anti-fator VIII da coagulação sangüínea

ABSTRACT:The participation of dermatophytic antigens in the host-parasite balance is still poorly understood. One of the difficulties encountered by researchers is the lack of dominant and specific antigens that can be used in such studies. In order to contribute to a better understanding of this aspect of infection, the present study identifies antigen fractions obtained from exoantigen and cytoplasmic extracts of Trichophyton mentagrophytes. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed the presence of 13 proteins in the exoantigen extract, whose molecular weight ranged from 12.5 to 90 kDa. The cytoplasmic extract contained 18 protein fractions ranging from 11 to 110 kDa. Immunoblotting showed the presence of immunodominant antigens against IgG, IgM and IgA antibodies. This affinity was observed in three proteins of the exoantigen extract and in three proteins of the cytoplasmic extract, with respective molecular weights of 33, 39 and 59, and 40, 55 and 82 kDa. These results are promising, especially when considering that these extracts contain antigenically distinct protein fractions which, once determined, may contribute to a better understanding of dermatophytoses, and may thus help in the development of alternative strategies for the diagnosis and treatment of this condition.

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“…The marked presence of IgG was expected since we used a longer immunization scheme for our animals (26).…”

Section: Resultsmentioning

confidence: 94%

Production of Trichophyton mentagrophytes antigens and their characterization in mice

Venturini¹,

Rossi-Ferreira²,

Arruda³

2008

J. Venom. Anim. Toxins incl. Trop. Dis

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“…O crio-precipitado não é uma opção terapêutica para portadores de hemofilia B, uma vez que não contém o fator IX (33). Foi utilizado até a década de 80, quando houve o surgimento do problema da contaminação pelo vírus do HIV, detectado no sangue de hemofílicos que receberam crio-precipitado plasmático (34).…”

Section: Tratamento E Acompanhamento Do Paciente Hemofílicounclassified

Hemofilias: Fisiopatologia, Diagnóstico E Tratamento

Colombo

Júnior

2013

Infarma

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Hemophilia is a serious hereditary blood coagulation disorder caused by a deficiency of clotting activity of factor VIII (hemophilia A) or IX (hemophilia B). The disease is caused by alterations in the coding genes of these factors located on the long arm of the sex chromosome X. Thus its occurrence in males is almost exclusive due to the man's presenting only one X chromosome. The severity of the disease can vary from severe, mild and moderately severe after dosage of the factors VIII and IX clotting. One of the most feared complications of patients with this disease refers to the development of inhibitors that are antibodies directed against the factors infused. The diagnosis is made through laboratory tests, mainly by coagulation profile and serum specific factors, based on the clinical manifestations of the patient. Hemophilia is not curable, and the basis of its treatment is the infusion of the deficient factor concentrate. However, patients may develop specific inhibitors to the factors infused and in these cases the treatment alternatives are the use of biotechnology to new drugs and the use of gene therapy.

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“…Hemodialysis in hemophiliacs with chronic renal failure is almost unknown in the literature. Hemophilia is a hereditary disease, linked to the X chromosome and characterized by a deficiency in factor VIII, causing a disturbance in the mechanism of blood coagulation 1. It manifests by susceptibility of the patient to episodes of spontaneous or post‐trauma bleeding 2.…”

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confidence: 99%

Management of a hemophilia patient in renal replacement therapy

Silva

Soares

Fráguas

et al. 2011

Dialysis & Transplantation

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We describe the case of a male hypertensive patient with severe hemophilia A. In August 1999 he was admitted to our nephrology department, with hemarthrosis, severe hypertension, dyspnea with minimal efforts, increasing blood urea nitrogen, anemia, uremic symptoms, reduced urine volume, mild edema of the lower limbs, and no hyperkalemia. Imaging confirmed the diagnosis of end‐stage renal disease. A Tenckhoff peritoneal dialysis catheter was inserted, and he began continuous ambulatory peritoneal dialysis. In August 2005 he evolved to peritoneal failure (peritoneal equilibration test showing ultrafiltration disorder I) and was transferred to hemodialysis. A permanent catheter was inserted into the right subclavian vein. Hemodialysis sessions lasted 4 hours, three times a week, and gradually resulted in hemodynamic stabilization. In September 2005, an arteriovenous fistula was placed in the right forearm between the cephalic vein and the radial artery. In January 2007 the patient was admitted with abdominal and epigastric pain, double‐lumen catheter infection, peritoneal catheter infection, globoid tympanic abdomen, and mild pain on palpation. Preliminary studies showed a large preperitoneal hematoma with bowel compression. Due to the catheter infection, we decided to puncture the fistula using a 17‐G needle. Apart from some bleeding during and after the beginning of hemodialysis, there were no other fistula complications. The patient had progressive worsening of clinical symptoms and died in February 2007. In summary, an individualized treatment plan, mainly adequate hemostatic monitoring, care of the dialysis access, and multiprofessional and family involvement, may help in the management of hemophilia patients undergoing dialysis. Dial. Transplant. © 2011 Wiley Periodicals, Inc.

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Obtenção e caracterização de anticorpo monoclonal murino anti-fator VIII da coagulação sangüínea

Cited by 3 publications

References 16 publications

Production of Trichophyton mentagrophytes antigens and their characterization in mice

Production of Trichophyton mentagrophytes antigens and their characterization in mice

Hemofilias: Fisiopatologia, Diagnóstico E Tratamento

Management of a hemophilia patient in renal replacement therapy

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