PURPOSE:To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal.
METHODS:This study was performed with New Zealand adult rabbits weighing 3.0 -4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS and Triton X-100 detergents), and enzymatic methods (DNase and RNase).
RESULTS:Generally, the cells surrounding soft tissues were successfully removed, but none protocol removed cells from the tracheal cartilage. However, longer protocols were more effective. The cost-benefits relation of the enzymatic processes was not favorable. It was possible to find out that the cartilaginous tissue submitted to the irradiation with LED 630nm and 475 nm showed an increased number of gaps without cells, but several cells were observed to be still present.
CONCLUSION:The light-emitting diode is a promising tool for decellularization of soft tissues.
Introduction: Tissue repair has been the ultimate goal of surgery. Cell culture requires a mechanical scaffold that supports cell growth and nutrient diffusion. Using platelet-rich plasma (PRP) as a 3D scaffold presents various advantages: it is a biological material, easily absorbed after transplantation, rich in growth factors, in particular, PDGF-ββ and TGF-β that stimulate extracellular matrix synthesis in cartilage culture. Objective: To develop a PRP 3D scaffold. Material and Methods: Two forms were idealized: Sphere and Carpet. Sterile conditions were used. The platelet gel remained in culture conditions, observed at an inverted microscope on a daily basis. Results: Both forms were successful because they produced a 3D
Entre os avanços da engenharia celular e biotecnologia nas últimas décadas, destaca-se a produção de anticorpos monoclonais murinos (AcMm) utilizados no aprimoramento diagnóstico nas rotinas laboratoriais. A produção de fator
IntroduçãoA hemofilia é uma doença causada pela deficiência do Fator VIII, uma glicoproteína que participa na via intrínse-ca da coagulação sangüínea e tem como etiologia a herança genética, ligada ao cromossomo X, ocorrendo exclusivamente em homens. A severidade da doença está diretamente relacionada com a extensão da deficiência de FVIII, que é classificada em hemofilia severa, moderada e branda. O tratamento consiste na reposição do Fator VIII através de transfusões sangüíneas.Em 1959, Pool & Robinson, 1 utilizando o método de Cohn 2 na produção de sedimento insolúvel (crioprecipitado) através de processos de congelamento e descongelamento lento, purificaram as proteínas plasmáticas com atividade anti-hemofílica. O crioprecipitado foi utilizado na terapia da hemofilia até a início da década de 80, quando surgiu o dramático problema da contaminação pelo vírus do HIV, detectado no sangue de hemofílicos que receberam crioprecipitado plasmático. A partir daí iniciaram-se inúmeras pesquisas com o objetivo de introduzir novos produtos na terapêutica da hemofilia. [3][4][5][6][7][8][9][10][11][12]
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