Observation of Brillouin scattering from single muscle fibres

We investigate the potential of Brillouin Light Scattering (BLS) Microspectroscopy for fast non-invasive all-optical assessment of the mechanical properties of viscose fibers and bleached softwood pulp. Using an optimized Brillouin spectrometer, we demonstrate fast spatial mapping of the complex longitudinal modulus over extended areas ([ 100 lm). Our results reveal that while the softwood pulp has a relatively uniform moduli, the viscous fibers have significant spatial heterogeneous in the moduli. Specifically, the viscose fibers exhibited a regular pattern of increasing and decreasing modulus normal to the fiber axis. The potential influence of a locally changing refractive index is investigated by holographic phase microscopy and ruled out. We discuss our results in light of the anisotropic mechanical properties of the fibers and are able to estimate the relative difference between the modulus along the fiber axis and that perpendicular to it. Results are presented alongside reference measurements of the quasi-static mechanical properties transverse to the fiber axes obtained using AFM-nanoindentation which reveal a similar trend, hinting at the potential usefulness of BLS for mechanical characterization applications. However, more detailed investigations are called for to uncover all the factors influencing the measured high-frequency BLS modulus and its significance in relation to physical properties of the fiber that may be of practical interest.

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“…It follows that the components of the latter can be calculated from the measured acoustic velocity (Eq. 1) via (Berne and Pecora 2000):…”

Section: Calculation Of Elastic Modulus From Bls Measurementsmentioning

confidence: 99%

Mechanical Properties of cellulose fibers measured by Brillouin spectroscopy

et al. 2020

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“…The muscle fibres used in this investigation were dissected from glycerinated rabbit psoas muscle, which was prepared by following the same procedure as Berovic et al (1989). Fibres were stored at-20°C for up to three months in a glycerinating solution consisting of 50% glycerol and 50% relaxing solution containing 3 mM ATP (Brenner 1983).…”

Section: Fibre Preparationmentioning

confidence: 99%

“…Fibres were stored at-20°C for up to three months in a glycerinating solution consisting of 50% glycerol and 50% relaxing solution containing 3 mM ATP (Brenner 1983). Single fibres were mounted inside an optical cell (Berovic et al 1989), which was filled with glycerinating solution. Fibres were carefully dissected in this solution without either twisting them or bending them unnecessarily.…”

Section: Fibre Preparationmentioning

confidence: 99%

Optical diffraction by well-ordered muscle fibres

1991

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We have studied the diffraction of a focussed laser beam by single fibres of glycerinated rabbit psoas muscle as a function of the angle of incidence. Diffraction efficiencies as high as 34% were observed at the first-order Bragg angle, indicative of well-ordered striated fibres with a strong periodic modulation of the refractive index. A theory is presented to account for our observations based upon the coupled-wave model developed by Kogelnik (1967) and Magnusson and Gaylord (1977) for the description of thick phase gratings in holography. We have solved the coupled-wave equations on a computer using a realistic index modulation taken from the measurements of Huxley and Hanson (1957). Comparison of theory with experiment shows that coupled-wave effects are indeed present in well-ordered muscle fibres, and the observed diffraction efficiency is in quite good agreement with what would be expected theoretically. Most importantly, the computer model allows us to calculate the diffraction efficiency for curved striations, which are observed for real muscle fibres under a microscope. The sensitivity of the diffraction efficiency to curvature of the striations may have implications for the interpretation of other optical experiments on muscle. We also consider the effects on our measurements of the focussing lens and refraction by the cylindrical fibre.

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“…Acoustically induced inelastic Brillouin light-scattering utilizing the advantages offered by confocal microscopy represents a non-invasive approach to determining the spatially resolved viscoelastic properties of biological tissue [16,17]. …”

Section: Introductionmentioning

confidence: 99%

Spatially resolved Brillouin spectroscopy to determine the rheological properties of the eye lens

Reiß¹,

Burau²,

Stachs³

et al. 2011

Biomed. Opt. Express

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Presbyopia is closely associated with the loss of accommodation, and hence with a decline in the viscoelastic properties of the human eye lens. In this article we describe a method for obtaining spatially resolved in vivo measurements of the rheological properties of the eye lens, based on the spectroscopic analysis of spontaneous Brillouin scattering using a virtually imaged phased array (VIPA). The multi-pass configuration enhances resolution to the extent that measurements are possible in elastic biological tissue characterized by intense scattering. We also present spatially resolved measurements obtained in extracted animal eyes and lenses. The results yield entirely new insights into the aging process of the eye lens.

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Observation of Brillouin scattering from single muscle fibres

Cited by 16 publications

References 13 publications

Mechanical Properties of cellulose fibers measured by Brillouin spectroscopy

Mechanical Properties of cellulose fibers measured by Brillouin spectroscopy

Optical diffraction by well-ordered muscle fibres

Spatially resolved Brillouin spectroscopy to determine the rheological properties of the eye lens

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