Objective Measurement of Sperm Motility Based Upon Sperm Penetration of Accudenz®

Froman, D. P.; McLean, Derek J.

doi:10.3382/ps.0750776

Cited by 88 publications

(63 citation statements)

References 31 publications

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“…In birds, the vaginal portion of oviduct regulates spermatozoa entry and only motile spermatozoa are able to traverse the vagina and enter into the hen's spermatozoa storage tubules (King et al 2000). As described by Froman and McLean (1996), spermatozoa motility is a primary determinant of fertility in the fowl. On the basis of results, a graded relationship was predicted between fertility and spermatozoa motility.…”

Section: Discussionmentioning

confidence: 99%

Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

Slanina¹,

Miškeje²,

Tirpák³

et al. 2018

Czech J. Anim. Sci.

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Slanina T., Miškeje M., Tirpák F., Błaszczyk M., Stawarz R., Massányi P. (2018): Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility. Czech J. Anim. Sci., 63, 127-135.The effect of taurine on the turkey spermatozoa motility and viability during the in vitro incubation was assessed. Experimental samples were prepared by diluting the raw semen in nine different concentrations of taurine -from 10 mg/ml to 0.078125 mg/ml. The motility parameters were evaluated by the CASA system (Computer Assisted Semen Analyser) using the program Sperm Vision ® and for spermatozoa viability assessment the eosin-nigrosin staining was performed. Selected parameters were evaluated at six time periods: 0, 1, 2, 3, 4, and 5 h at 5°C and 41°C. At 5°C, a significantly lower percentage of motility and progressive motility was detected only in the samples with the highest concentration of taurine (10 mg/ml) at time 0 and 1. After 2 h of incubation a significant preventive effect of taurine on spermatozoa parameters was observed. The tendency of the taurine effect on motility parameters was different during the in vitro incubation at 41°C. Significantly lower values of motility parameters were detected in all experimental samples in comparison to the control after 5 h. The analysed concentrations of taurine did not significantly affect viability of turkey spermatozoa during all time periods. A higher percentage of dead spermatozoa were observed at 41°C (4.87-9.90%) if compared to 5°C (2.12-4.88%). The results indicated that the addition of taurine (from 2.5 to 7.5 mg/ml) to turkey spermatozoa positively affected the monitored spermatozoa parameters incubated at 5°C.

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Section: Discussionmentioning

confidence: 99%

Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

Slanina¹,

Miškeje²,

Tirpák³

et al. 2018

Czech J. Anim. Sci.

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“…Sperm mobility was measured using the Sperm Mobility Test developed by Froman and McLean (1996) and modified for the turkey . Semen concentration was determined using a spectrophotometer validated for poultry semen (IMV Microreader I, Minneapolis, MN, USA).…”

Section: Evaluation Of Sperm Mobility Of Tomsmentioning

confidence: 99%

Field testing the influence of sperm competition based on sperm mobility in breeder turkey toms

Donoghue

Kirby

Froman

et al. 2003

British Poultry Science

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1. Commercial reproduction of turkeys relies on pooling of semen from multiple males for inseminations. Understanding how sperm characteristics influence paternity under commercial breeding conditions is important to improving production efficiency. 2. The objective of this study was to evaluate progeny production of individual toms following commercial practices of pooling semen to determine if sperm mobility influences progeny production in field conditions. 3. A total of 104 toms were evaluated for sperm mobility. A subset of 10 toms were housed together and semen was collected, pooled and used to inseminate hens (n = 28). Hens were inseminated at 30 weeks of age and weekly thereafter. 4. Ejaculates from each tom were evaluated on two separate days for sperm mobility. Semen from each tom was diluted and layered upon 6% (wt/vol) Accudenz solution. The sperm suspension was incubated at 41 degrees C for 5 min and absorbance was measured with a spectrophotometer. 5. Toms were ranked by absorbance and categorised as high or low if mobility score was +/- 1 SD from the flock mean (average). 6. For parentage determination, DNA was extracted from tom, hen and poult blood. Poult parentage (n = 276) was determined at one day of age or at 14 weeks by analysis of marker genotypes that were generated by polymerase chain reaction (PCR) amplification of genomic DNA with selected microsatellite markers. 7. Sperm mobility differed across males with absorbance values ranging from 0.147 to 0.366. 8. Findings demonstrate differences in poult production among individual toms when semen from multiple males was pooled and inseminated. Toms classified as high, average and low produced 55, 41 and 4% of the offspring, respectively. 9. It appears that sperm mobility is a trait that influences sperm competition among toms under field conditions where sperm numbers inseminated from individual toms are not controlled or constant and that toms with low sperm mobility produce few offspring.

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“…Sperm motility was measured with an objective spectrophotometric method that quantitates the number of sperm that enter a 6% (w:v) Accudenz solution [15]. Semen was obtained from individual males (n = 40 males per genotype), and sperm concentration was determined.…”

Section: Evaluation Of Sperm Motility By Sperm Penetration Of Accudenzmentioning

confidence: 99%

Reduced Glucose Transport in Sperm from Roosters (Gallus domesticus) with Heritable Subfertility1

McLean

Jones

Froman

1997

Biology of Reproduction

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Roosters homozygous for the rose comb allele (R/R) are subfertile. In previous research, these subfertile roosters were characterized by an in vitro sperm penetration assay as having limited sperm motility. The objectives in the present study were to characterize sperm motility by computer-assisted sperm motion analysis and to account for a mechanism underlying poor sperm motility. Percentages of motile sperm differed between subfertile males and fertile controls (r/r) by 29% (p < 0.001). The concentration of intracellular ATP in sperm form subfertile roosters was less than in that from fertile controls (p < 0.001). The genotypic difference is sperm motility, as measured with the sperm penetration assay, was maintained when ATP production was dependent on anaerobic glycolysis (p < 0.001). In this case, sperm were incubated with exogenous glucose and cyanide. Consequently, we could not attribute the genotypic difference in sperm mobility to mitochondrial respiration. In contrast, glucose transport, as measured by the uptake of [1,2-3H]-2-deoxy-D-glucose, was reduced in sperm from subfertile roosters (p < 0.001). Neither hexokinase nor glyceraldehyde-3-phosphate dehydrogenase activity differed between genotypes (p > 0.05). Likewise, lactate dehydrogenase activity did not differ between genotypes (p > 0.05). As evidenced by creatine kinase activity and dynein ATPase activity, neither the potential for energy transfer nor utilization within the axoneme differed between genotypes (p > 0.05). Therefore, we attribute the subfertility of roosters homozygous for the rose comb allele to decreased spermatozoal glucose transport.

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Objective Measurement of Sperm Motility Based Upon Sperm Penetration of Accudenz®

Cited by 88 publications

References 31 publications

Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

Field testing the influence of sperm competition based on sperm mobility in breeder turkey toms

Reduced Glucose Transport in Sperm from Roosters (Gallus domesticus) with Heritable Subfertility1

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