Reduced Glucose Transport in Sperm from Roosters (Gallus domesticus) with Heritable Subfertility1

McLean, Derek J.; Jones, Louis G.; Froman, D. P.

doi:10.1095/biolreprod57.4.791

Cited by 11 publications

(5 citation statements)

References 36 publications

(55 reference statements)

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“…Glucose uptake was determined using radiolabeled [1,2-3 H]-2-deoxy-Dglucose [18]. Sperm were incubated 30 min at RT with 0.5 lCi [1,2-3 H]-2deoxy-D-glucose (52 Ci/mmol) in 1 ml modified HEPES-TYH medium without glucose.…”

Section: Glucose Uptakementioning

confidence: 99%

Lactate Dehydrogenase C and Energy Metabolism in Mouse Sperm

Odet

Gabel

Williams

et al. 2011

Biology of Reproduction

106

103

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We demonstrated previously that disruption of the germ cell-specific lactate dehydrogenase C gene (Ldhc) led to male infertility due to defects in sperm function, including a rapid decline in sperm ATP levels, a decrease in progressive motility, and a failure to develop hyperactivated motility. We hypothesized that lack of LDHC disrupts glycolysis by feedback inhibition, either by causing a defect in renewal of the NAD(+) cofactor essential for activity of glyceraldehyde 3-phosphate dehydrogenase, sperm (GAPDHS), or an accumulation of pyruvate. To test these hypotheses, nuclear magnetic resonance analysis was used to follow the utilization of labeled substrates in real time. We found that in sperm lacking LDHC, glucose consumption was disrupted, but the NAD:NADH ratio and pyruvate levels were unchanged, and pyruvate was rapidly metabolized to lactate. Moreover, the metabolic disorder induced by treatment with the lactate dehydrogenase (LDH) inhibitor sodium oxamate was different from that caused by lack of LDHC. This supported our earlier conclusion that LDHA, an LDH isozyme present in the principal piece of the flagellum, is responsible for the residual LDH activity in sperm lacking LDHC, but suggested that LDHC has an additional role in the maintenance of energy metabolism in sperm. By coimmunoprecipitation coupled with mass spectrometry, we identified 27 proteins associated with LDHC. A majority of these proteins are implicated in ATP synthesis, utilization, transport, and/or sequestration. This led us to hypothesize that in addition to its role in glycolysis, LDHC is part of a complex involved in ATP homeostasis that is disrupted in sperm lacking LDHC.

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Section: Glucose Uptakementioning

confidence: 99%

Lactate Dehydrogenase C and Energy Metabolism in Mouse Sperm

Odet

Gabel

Williams

et al. 2011

Biology of Reproduction

106

103

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“…Gradually it was elucidated that defective sperm motility in roosters homozygous for Rose-comb is the cause of the observed poor fertility and duration of fertility [9]–[15]. This reduced motility is thought to result in sperm from a homozygous Rose-comb rooster ( RR ) being outcompeted by sperm from roosters carrying the wild-type allele ( Rr and rr ) in promiscuous mating or heterospermic insemination experiments [16], [17].…”

Section: Introductionmentioning

confidence: 99%

The Rose-comb Mutation in Chickens Constitutes a Structural Rearrangement Causing Both Altered Comb Morphology and Defective Sperm Motility

et al. 2012

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Rose-comb, a classical monogenic trait of chickens, is characterized by a drastically altered comb morphology compared to the single-combed wild-type. Here we show that Rose-comb is caused by a 7.4 Mb inversion on chromosome 7 and that a second Rose-comb allele arose by unequal crossing over between a Rose-comb and wild-type chromosome. The comb phenotype is caused by the relocalization of the MNR2 homeodomain protein gene leading to transient ectopic expression of MNR2 during comb development. We also provide a molecular explanation for the first example of epistatic interaction reported by Bateson and Punnett 104 years ago, namely that walnut-comb is caused by the combined effects of the Rose-comb and Pea-comb alleles. Transient ectopic expression of MNR2 and SOX5 (causing the Pea-comb phenotype) occurs in the same population of mesenchymal cells and with at least partially overlapping expression in individual cells in the comb primordium. Rose-comb has pleiotropic effects, as homozygosity in males has been associated with poor sperm motility. We postulate that this is caused by the disruption of the CCDC108 gene located at one of the inversion breakpoints. CCDC108 is a poorly characterized protein, but it contains a MSP (major sperm protein) domain and is expressed in testis. The study illustrates several characteristic features of the genetic diversity present in domestic animals, including the evolution of alleles by two or more consecutive mutations and the fact that structural changes have contributed to fast phenotypic evolution.

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“…Sperm motility in chickens correlates with glucose uptake used for sperm motility. These results are based on the study reported by (McLean et al 1997) that single-combed chickens absorb glucose better than rosy-combed chickens. According to McGary et al (2002), broadcombed chickens have better fertility than small-combed chickens.…”

Section: Quality Of Fresh Kub Semen Quality With Different Phenotypementioning

confidence: 79%

Effect of Carbohydrate Type and Phenotype on the Quality of Post-Thawing Frozen Semen of KUB Chicken

Maghfira,

Karja,

Arifiantini

et al. 2023

JITV

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The superior Balitbangtan Kampung Chicken (KUB) chickens have different phenotypes. It was reported that the chicken phenotype was related to semen quality. This study aimed to determine the post-thawing characteristics and quality of KUB chicken semen with different phenotypes frozen in Ringer's lactate egg yolk (RLEY) diluent with the addition of fructose or glucose. Semen was collected using the massaging method from 20 KUB chickens with a single comb phenotype and black or dark brown feather color with a red feather neck (SCNR), green-black single comb with white feather neck collar (SCNW), pea comb and black feathers or dark brown fur with a red neck (PCNR), and a green-black pea comb with a white neck (PCNW). Semen from each chicken phenotype was divided into three parts or frozen in three types of diluents: RLEY, RLEY+fructose (RLEYF), and RLEY+glucose (RLEYG). The highest sperm motility was found in the diluent with the addition of glucose in the SCNR and PCNW phenotypes (P0.05). The highest sperm viability was shown in the RLEYG diluent in the PCNW phenotype (P0.05). The highest abnormality was found in the RLEY and RLEYF diluents in the SCNW, PCNR, and PCNW groups, whereas in the RLEYG group, it was only found in the PCNR group. From the results of this study, it can be concluded that the type of glucose and chicken phenotype influences the quality of post-thawing semen. The best is found in diluents with glucose attachments in the SCNR phenotype.

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Reduced Glucose Transport in Sperm from Roosters (Gallus domesticus) with Heritable Subfertility1

Cited by 11 publications

References 36 publications

Lactate Dehydrogenase C and Energy Metabolism in Mouse Sperm

Lactate Dehydrogenase C and Energy Metabolism in Mouse Sperm

The Rose-comb Mutation in Chickens Constitutes a Structural Rearrangement Causing Both Altered Comb Morphology and Defective Sperm Motility

Effect of Carbohydrate Type and Phenotype on the Quality of Post-Thawing Frozen Semen of KUB Chicken

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