Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

Abstract

Slanina T., Miškeje M., Tirpák F., Błaszczyk M., Stawarz R., Massányi P. (2018): Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility. Czech J. Anim. Sci., 63, 127-135.The effect of taurine on the turkey spermatozoa motility and viability during the in vitro incubation was assessed. Experimental samples were prepared by diluting the raw semen in nine different concentrations of taurine -from 10 mg/ml to 0.078125 mg/ml. The motility parameters were evaluated by the CASA system (Computer Assisted Semen Analyser) using the program Sperm Vision ® and for spermatozoa viability assessment the eosin-nigrosin staining was performed. Selected parameters were evaluated at six time periods: 0, 1, 2, 3, 4, and 5 h at 5°C and 41°C. At 5°C, a significantly lower percentage of motility and progressive motility was detected only in the samples with the highest concentration of taurine (10 mg/ml) at time 0 and 1. After 2 h of incubation a significant preventive effect of taurine on spermatozoa parameters was observed. The tendency of the taurine effect on motility parameters was different during the in vitro incubation at 41°C. Significantly lower values of motility parameters were detected in all experimental samples in comparison to the control after 5 h. The analysed concentrations of taurine did not significantly affect viability of turkey spermatozoa during all time periods. A higher percentage of dead spermatozoa were observed at 41°C (4.87-9.90%) if compared to 5°C (2.12-4.88%). The results indicated that the addition of taurine (from 2.5 to 7.5 mg/ml) to turkey spermatozoa positively affected the monitored spermatozoa parameters incubated at 5°C.

Exogenous Factors Affecting the Functional Integrity of Male Reproduction

“…The exposure to oxidative stress may result in adaptation (increased activity of defense mechanisms), damage to macromolecules (DNA fragmentation, protein modifications, lipid peroxidation) [ 172 , 173 ], or cell death (apoptosis, or even necrosis with a wider impact on surrounding cells and tissue) [ 174 ]. The effect of OS may be also mitigated by antioxidants by oral administration [ 175 , 176 , 177 , 178 ] or direct supplementation of reproduction-associated cells [ 179 , 180 , 181 , 182 , 183 ].…”

Exposure to non‐ionizing electromagnetic radiation of public risk prevention instruments threatens the quality of spermatozoids

“…Ejaculates extended with cryopreservative medium and radiated prior to/post‐freezing were analysed in time periods: 0 and 60 min and were kept at the same incubation temperature. Following the thawing in a 37°C water bath for 20 s, each of prepared samples was evaluated using a CASA system—Sperm Vision ® program (Minitub, Tiefenbach, Germany) equipped with a microscope (Olympus BX 51, Japan; Roychoudhury & Massanyi, ; Slanina, Miškeje, Tirpák, Błaszczyk, Stawarz, et al, ).…”

Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality

“…Using the species-specific set-up, the basic and the most important parameters were selected-total motile spermatozoa (MOT; %), progressively motile spermatozoa (PRO; %), velocity curved line (VCL; µm/s), amplitude of lateral head displacement (ALH; µm), and beat cross frequency (BCF; Hz)-and were evaluated using the computer-assisted semen analyzer (CASA) Sperm Vision ® program (Minitube, Tiefenbach, Germany) equipped with a microscope (Olympus BX 51, Olympus Corporation, Tokyo, Japan) [37]. Within each of the CASA assessments, several different fields of view of Makler Counting Chamber were evaluated as described by Slanina et al [38] and Tirpak et al [39]. To distinguish the time of the analysis, every parameter is labeled with an underscore (_) and the time of the measurement (0-initial analysis; 1-analysis after an hour incubation at 6 • C) (e.g., MOT_0, MOT_1, etc.…”