o-toluoylcholine as substrate for measurement of serum pseudo-cholinesterase activity

Okabe, Hiroaki; Kita, Masami; Netsu-Nakayama, K; Yamamoto, Tamotsu; Watanabe, Kinuyo; Shimizu, Yasuko; Noma, Akio

doi:10.1016/0009-8981(80)90154-0

Cited by 8 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Choline oxidase, peroxidase, phenol and 4aminoantipyrine (6,15) were obtained from Kyowa Hakko Kyogyo Co. Acetyl-, butyryl-, and propionyl-thiocholine iodide, 2,2'-dithiodipyridine (16) and the other regents used were purchased from Nakarai Chem. Co., respectively.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Substrates for Measuring Serum Choline Esterase Activity in Hepato-Biliary Disease

Uete¹,

Masui²,

Miyauchi³

1985

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

The efficacy of various Substrates for measuring serum choline esterase for the evaluation of hepatic function was studied using 0-toluoyl-and succinyl-choline, and acetyl, butyryl-and propionylthiocholine. In hepatic disease, the serum choline esterase activity with these Substrates was decreased at a similar rate, showing no significant difference. In 78 -84% of cases with hepatic cirrhosis the enzyme activity with these Substrates was less than 50% of the average level of normal individuals, but in acute and chronic hepatitis only 4-9 and 12-14% of patients showed these lower values, respectively. The present study indicates the usefulness of sequential monitoring of serum choline esterase activity with any of these Substrates for assessing hepatic disease, particularly cirrhosis, and for monitoring the course of hepatic disease.

show abstract

Section: Methodsmentioning

confidence: 99%

Comparison of Substrates for Measuring Serum Choline Esterase Activity in Hepato-Biliary Disease

Uete¹,

Masui²,

Miyauchi³

1985

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…Serum ChE activity was determined by the enzymatic method using a Determiner ChE-S® assay kit (Kyowa Hakko Kogyo Co. Ltd., Tokyo) (8). Choline was liberated from the substrates (o-toluoylcholine) by ChE and oxidized by choline oxidase to betaine with the simultaneous production of hydrogen peroxide, which was determined using 4-aminoantipyrine and phenol in the presence of peroxidase.…”

Section: ) Measurement Of Serum Che Activitymentioning

confidence: 99%

Hereditary Serum Cholinesterase Deficiency Associated with Severe Lipid Deposition in the Kidney.

et al. 1993

View full text Add to dashboard Cite

A 47-year-old womanwho was homozygousfor a silent cholinesterase gene (hereditary serum cholinesterase deficiency) presented with nephrotic syndrome and hyperlipidemia. Renal biopsy performed in 1986 demonstrated mesangial proliferative glomerulonephritis. Four years later, a second biopsy revealed progression with mesangial interpositions and severe lipid deposition in the glomeruli, tubules and interstitium. This is the first case of hereditary serum cholinesterase deficiency accompanied by renal disease. Serum cholinesterase deficiency may be related to hyperlipidemia and abnormal lipid deposition in the kidney, which promotes the progression of renal disease. (Internal Medicine 32: 145-151 1993)

show abstract

A procedure for the kinetic colorimetric determination of serum cholinesterase activity

Artiss

McGowan

Strandbergh

et al. 1982

Clinica Chimica Acta

View full text Add to dashboard Cite

o-toluoylcholine as substrate for measurement of serum pseudo-cholinesterase activity

Cited by 8 publications

References 8 publications

Comparison of Substrates for Measuring Serum Choline Esterase Activity in Hepato-Biliary Disease

Comparison of Substrates for Measuring Serum Choline Esterase Activity in Hepato-Biliary Disease

Hereditary Serum Cholinesterase Deficiency Associated with Severe Lipid Deposition in the Kidney.

A procedure for the kinetic colorimetric determination of serum cholinesterase activity

Contact Info

Product

Resources

About