“…Barham and Trinder (1972) suggested to replace phenol by 2-hydroxy-3,5-dichlorobenzene sulfonate (HDCBS), which would achieve a raise in sensitivity by 4 to 5 fold. This reagent was used successfully for quantification of glucose, triglycerides, creatine kinase and choline esterase in coupled enzyme reactions (Artiss et al, 1982, McGowan et al, 1983, Wimmer et al, 1985. So far there is no experience concerning application in EIA.…”