Nutrition of the micropropagated fruit trees in vitro and ex vitro

Balla, I.; Vértesy, J.; Végváry, Gy.; Szűcs, E.; Kállay, T.; Vörös, I.; Bíró, Borbála

doi:10.31421/ijhs/9/2/390

Cited by 5 publications

(2 citation statements)

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“…Varying sucrose concentrations in a rooting medium can positively affect root induction and development. For example, the in vitro shoots of Astragalus chrysochlorus failed to root in MS medium containing 30 g L −1 of sucrose, but rooting was strongly stimulated (93% of shoots) when the sucrose concentration was reduced to 20 g L −1 [33]. In our study, no rooting occurred on medium without sucrose under all different LED treatments.…”

Section: Effects Of Light Spectra and Sucrose Treatments And Their In...contrasting

confidence: 41%

Effects of Artificial Light Spectra and Sucrose on the Leaf Pigments, Growth, and Rooting of Blackberry (Rubus fruticosus) Microshoots

et al. 2022

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Light emitting diodes (LEDs) are potential light sources for in vitro plant cultures. Here, axillary blackberry shoots were grown in MS medium with indole-3-butyric acid (1 mg L−1), naphthalene acetic acid (0.5 mg L−1), and sucrose supplementation (0–60 g L−1) and the cultures were incubated under four light treatments: three LED light treatments (blue + red light (2:1 spectral ratio), blue + red light (1:2), and cool + warm white light (1:1)) and a standard florescent tube white spectrum treatment. Sucrose was indispensable for rooting of blackberry microshoots. Sucrose concentrations up to 45 g L−1 increased total root length and root surface area under all light treatments. However, at this sucrose concentration, leaf area and vegetative growth were negatively affected. Plantlets grown in media containing 15–30 g L−1 of sucrose exhibited the highest leaf pigments, shoot length, and number of leaves. LED treatments increased leaf pigments as compared with florescent treatment. Plantlets grown under blue + red light (2:1) had the highest stoma aperture length and width, whereas cool + warm white light resulted in the lowest values. Among the LED treatments, blue + red light (2:1) resulted in the highest leaf area, chlorophyll and carotenoid contents, and vegetative growth, whereas fluorescent resulted in the lowest values. A combination of blue and red light at a 2:1 spectral ratio with 30 g L−1 of sucrose is recommended for the optimal in vitro rooting and vegetative growth of blackberry microshoots.

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Section: Effects Of Light Spectra and Sucrose Treatments And Their In...contrasting

confidence: 41%

Effects of Artificial Light Spectra and Sucrose on the Leaf Pigments, Growth, and Rooting of Blackberry (Rubus fruticosus) Microshoots

et al. 2022

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“…Inducing Malus seiboldii genotypes at night with 25 M IBA in liquid or agarized medium increased rooting % (51). Various concentrations of IBA initiate roots, but 2.0 and 2.5 mg/L in MM106 show the most (68). At 8+5 weeks, X0.5 and X1.0 MS medium with 14.70 m IBA showed the highest rooting response (20.8% and 75.9%).…”

Section: In Vitro Rootingmentioning

confidence: 97%

Micropropagation of fruit crops: a review

et al. 2022

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In vitro culture has the greatest commercial value in the extremely quick creation of clonal plants compared to conventional techniques of propagation. It has also proven to be of tremendous practical utility as assistance to the development and maintenance of disease-free planting material, germplasm conservation, and supplements to the regular methods of plant progress, such as plant genetic engineering. The discovery and implementation of current tissue culture techniques are thought to be paving the way for a second green revolution. Here, the standardization of techniques for the in vitro clonal propagation of fruit crops is highlighted. In addition, we summarize the effects of several surface sterilizing agents, plant growth regulators (PGRs), and anti-phenolic chemicals on culture establishment, shoot proliferation, in vitro rooting, hardening and economics of in vitro generation of true-to-type plants.

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