Nutrient metabolism and utilization in the liver

Danfær, A.

doi:10.1016/0301-6226(94)90163-5

Cited by 63 publications

(50 citation statements)

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“…Furthermore, GOx is inversely correlated to milk production . The GOx obtained in the present study supports previous measurements (Hammon et al, 2008), and the increasing rate of GOx with lactation is in line with increasing eGP and glucose availability (Danfaer, 1994;Bell and Bauman, 1997). Glucose oxidation was numerically lower in CLA-than Ctrl-fed cows, which seems to support the hypothesis of lower glucose use in CLA-fed cows.…”

Section: Cla Effects On Glucose Metabolismsupporting

confidence: 92%

“…Endogenous glucose production increased with ongoing lactation in both groups, supporting the concept of increased eGP with increased DMI in ruminants (Bell and Bauman, 1997;Brockman, 2005;Aschenbach et al, 2010). The amount of available glucose is important to achieve the full genetic potential for milk production, because lactose is the major osmoregulator for mammary uptake of water (Linzell, 1972;Danfaer, 1994;Rigout et al, 2002).…”

Section: Cla Effects On Glucose Metabolismsupporting

confidence: 51%

“…On the other hand, lactose output increased with CLA feeding, as more glucose was available for lactose synthesis, despite eGP reduction in CLA-fed cows. We assume a higher lactose synthesis due to higher glucose availability because of less glucose use for milk fat synthesis in CLA-fed cows and therefore a greater milk yield in CLA-fed cows (Linzell, 1972;Danfaer, 1994;Rigout et al, 2002). In contrast to long-chain FA in rumen-protected fat, the trans-10,cis-12 CLA isomer inhibits milk fat synthesis in the mammary gland, resulting in a reduced milk fat content; whereas rumen-protected long-chain FA feeding did not inhibit milk fat content, but reduced de novo FA synthesis and increased milk fat synthesis from long-chain FA (Grummer and Carroll, 1991;Bauman et al, 2008).…”

Section: Cla Effects On Glucose Metabolismmentioning

confidence: 99%

“…With the onset of lactation, GOx decreases dramatically in dairy cows, because cows reduce GOx in muscle and fat tissue during early lactation to provide sufficient glucose for milk synthesis (Danfaer, 1994;Bell and Bauman, 1997). Furthermore, GOx is inversely correlated to milk production .…”

Section: Cla Effects On Glucose Metabolismmentioning

confidence: 99%

“…In ruminants, very little glucose originates from net portal absorption, and endogenous glucose production (eGP); that is, the sum of gluconeogenesis and glycogenolysis, provides most of the glucose for milk production (Danfaer, 1994;Bell and Bauman, 1997;Brockman, 2005;Aschenbach et al, 2010). Therefore, hepatic glucose production strongly increases after calving in dairy cows to provide glucose (Danfaer, 1994;Bell and Bauman, 1997;Brockman, 2005;Aschenbach et al, 2010). However, it is not known if CLA supplementation affects eGP as well as glucose oxidation (GOx) in high-yielding dairy cows during early lactation.…”

Section: Introductionmentioning

confidence: 99%

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Supplementation of conjugated linoleic acid in dairy cows reduces endogenous glucose production during early lactation

Hötger

Hammon

Weber

et al. 2013

Journal of Dairy Science

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Trans-10,cis-12 conjugated linoleic acid (CLA) supplementation causes milk fat depression in dairy cows, but CLA effects on glucose metabolism are not clear. The objective of the study was to investigate glucose metabolism, especially endogenous glucose production (eGP) and glucose oxidation (GOx), as well as hepatic genes involved in endogenous glucose production in Holstein cows supplemented either with 50 g of rumen-protected CLA (9% trans-10,cis-12 and 10% cis-9,trans-11; CLA; n = 10) or 50 g of control fat (24% C18:2; Ctrl; n = 10) from wk 2 before parturition to wk 9 of lactation. Animal performance data were recorded and blood metabolites and hormones were taken weekly from 2 wk before to 12 wk after parturition. During wk 3 and 9 after parturition, glucose tolerance tests were performed and eGP and GOx were measured by [U-13 C] glucose infusion. Liver biopsies were taken at the same time to measure total fat and glycogen concentrations and gene expression of pyruvate carboxylase, cytosolic phosphoenolpyruvate carboxykinase, glucose-6-phosphatase, and carnitine palmitoyl-transferase 1. Conjugated linoleic acid feeding reduced milk fat, but increased milk lactose output; milk yield was higher starting 5 wk after parturition in CLA-fed cows than in Ctrl-fed cows. Energy balance was more negative during CLA supplementation, and plasma concentrations of glucose were higher immediately after calving in CLAfed cows. Conjugated linoleic acid supplementation did not affect insulin release during glucose tolerance tests, but reduced eGP in wk 3, and eGP and GOx increased with time after parturition. Hepatic gene expression of cytosolic phosphoenolpyruvate carboxykinase tended to be lower in CLA-fed cows than in Ctrl-fed cows. In spite of lower eGP in CLA-fed cows, lactose output and plasma glucose concentrations were greater in CLA-fed cows than in Ctrl-fed cows. This suggests a CLA-related glucose sparing effect most likely due to lower glucose utilization for milk fat synthesis and probably because of a more efficient whole-body energy utilization in CLA-fed cows.

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Section: Cla Effects On Glucose Metabolismsupporting

confidence: 92%