Nutrient culture media with agar is effective for early and rapid screening of iron toxicity tolerance in rice

Nugraha, Yudhistira; Ardie, Sintho Wahyuning; Ghulammahdi, Munif; Suwarno, &; Aswidinnoor, Hajrial

doi:10.1007/s12892-015-0075-z

Cited by 15 publications

(17 citation statements)

References 31 publications

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“…Two-week-old seedlings were grown on the other container (35 × 28.5 × 12 cm 3 ) containing 8 L half-strength Yoshida's solution (pH = 4.0). In each nutrient culture solution, 0.2% (g/v) agar was added according to Nugraha et al, 25 and Turhadi, 26 to prevent the excessive oxidation of Fe. Two types of treatments (0 ppm as control and 400 ppm Fe in the form of…”

Section: Plant Materials and Experiments Conditionsmentioning

confidence: 99%

Iron toxicity-induced physiological and metabolite profile variations among tolerant and sensitive rice varieties

Turhadi

Hamim

Ghulamahdi

et al. 2019

Plant Signaling & Behavior

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Iron toxicity stress causes physiological and metabolic changes in rice and other plants. To date, there is little information about the metabolite profile of rice under Fe toxicity conditions. In fact, metabolite has a contribution to the physiological condition of plants. Plant metabolomics is a study of low-molecular weight metabolites in plants under certain conditions. The objective of the research was to investigate physiological and metabolic changes in rice under Fe toxicity stress. Two-week-old seedlings of four rice varieties with various Fe toxicity tolerance levels were stressed hydroponically with 400 ppm FeSO 4. 7H 2 O for 10 d. Numerous physiological characters were observed and untargeted metabolomic analysis was carried out using gas chromatography-mass spectrophotometry (GC-MS). The results showed Fe toxicity induced physiological and metabolite variation in rice. By comparing the metabolites synthesized in Fe toxicity-stressed plants with control plants, it showed that elaidic acid, linoleic acid, and linolenic acid could be as metabolite marker candidates for rice response to Fe toxicity stress. When plants exposed to Fe toxicity stress, elaidic acid increased, whereas linoleic-and linolenic acid decreased. The alteration of fatty acid composition in the root and shoot suggests the alteration of metabolites is one of the tolerance strategies of rice to Fe toxicity stress. This finding offers an insight about the tolerance strategies of rice under Fe toxicity stress related to the maintenance process of the cell membranes during this stress. The genes underlying biosynthesis of the fatty acid could be a target of future research on responsible genes for Fe toxicity tolerance in rice.

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Section: Plant Materials and Experiments Conditionsmentioning

confidence: 99%

Iron toxicity-induced physiological and metabolite profile variations among tolerant and sensitive rice varieties

Turhadi

Hamim

Ghulamahdi

et al. 2019

Plant Signaling & Behavior

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“…The plastic trays were filled with pre-culture solution using 1 L of 8×strength stock nutrient solution (Yoshida solution) added with 7 L of deionized water. After 14 days the preculture media solutions were replaced by new Yoshida solution with addition of 400 mg L-1 of Fe 2+ supplied as FeSO4 and a 0.2 % agar, to minimize oxidation of ferrous iron (Nugraha et al 2016). The initial pH was adjusted at 5.5 (±0.2).…”

Section: Plant Materials and Media Culturementioning

confidence: 99%

Responses of Selected Indonesian Rice Varieties under Excess Iron Condition in Media Culture at Seedling Stage

Nugraha¹,

Rumanti²,

Guswara³

et al. 2016

j. penelit. pertan. tanam. pangan

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Iron toxicity could limit rice productivity on irrigated lowland acid and swampy soil. The use of iron toxicity tolerant rice is an alternative strategy to improve rice productivity in these areas. We studied the phenotypic variation of twenty-four rice genotypes and characterized the fate of Fe2+ along its path between the roots and shoot of rice plant. Twenty-four rice genotypes form different agro-ecosystem were grown under agar nutrient solution conditions with 400 mg. L-1 iron stress and under normal condition. We found variation in the biomass accumulation of rice seedling during stress of iron namely, high accumulated biomass tolerant type and low accumulate biomass tolerant type. The relative biomass weight was highly correlated with the leaf bronzing scores (LBS) under excess iron. Based on these categorizations, we chose six genotypes to observe the present of Fe in root and shoot using invivo-staining 2,2 bypiridine. The result indicated that some genotypes were able to develop root and shoot aerenchym during iron stress. This was related to the development on root iron plaque and the iron content of the shoot of the rice seedling. In this present study, rice genotypes could be classified as the includer tolerant type (Inpara 2) and some others were the excluder tolerant type (Mahsuri, Pokkali and Siam Saba). This information on tolerance strategies is important for rice breeder to develop physiological-based breeding program of iron-toxicity tolerant in rice.

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“…However, less than 1 million ha has been used for rice cultivation. Moreover, the productivity of rice in these areas was low, with only 2-4 t/ha (Hairmansis et al 2013;Nugraha et al 2016), compared to the national productivity of 5.1 t/ha (http://www.bps.go.id/tnmn_ pgn.php).…”

Section: Introductionmentioning

confidence: 99%

Development of High-Yielding Rice Varieties Suitable for Swampy Lands in Indonesia

Rumanti¹,

Nugraha²,

Wening³

et al. 2016

Plant Breed. Biotech.

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Productivity of swampy land rice varieties in Indonesia remains low due to abiotic and biotic stresses. Iron toxicity, submergence and long-term flooding are common problems that affect plant growth, sometimes leading to total crop loss. Meanwhile, rice blast, rice tungro virus (RTV), and bacterial leaf blight (BLB) can also lead to severe yield loss. These stresses could be overcome by an integrative approach of cultural practices and planting multi-stress tolerant rice. Here we describe the development of new high-yielding varieties that are adapted to the swampy land environments in Indonesia with multi-stress tolerance to diverse abiotic and biotic stresses. Multi-environmental trials were performed in nine locations to screen for several abiotic and biotic stresses in the field and greenhouse in 2012 and 2013. This study identified promising rice lines that had high grain yield as well as tolerance to iron toxicity and submergence, resistance to BLB and RTV, and good grain quality. The best line was B11377F-MR-34-2, derived from a double cross among Cinglonik, IRBB7, Mamberamo and IR64. It was confirmed that this advanced breeding line carries the submergence tolerance gene, SUB1, through expression analysis of the SUB1A gene. Another promising line was B11586F-MR-11-2-2, having iron toxicity tolerance, resistance to RTV, and high milling recovery. Moreover, these lines have passed the Indonesian national variety release committee and have been named as Inpara8 and Inpara9, and are targeted for dissemination and adoption in the swampy areas.

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Nutrient culture media with agar is effective for early and rapid screening of iron toxicity tolerance in rice

Cited by 15 publications

References 31 publications

Iron toxicity-induced physiological and metabolite profile variations among tolerant and sensitive rice varieties

Iron toxicity-induced physiological and metabolite profile variations among tolerant and sensitive rice varieties

Responses of Selected Indonesian Rice Varieties under Excess Iron Condition in Media Culture at Seedling Stage

Development of High-Yielding Rice Varieties Suitable for Swampy Lands in Indonesia

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