Nutrient availability as an arbiter of cell size

Kellogg, Douglas R.; Levin, Petra Anne

doi:10.1016/j.tcb.2022.06.008

Cited by 20 publications

(10 citation statements)

References 72 publications

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“…In the present study, we provided evidence that the intracellular c-di-GMP level was important for cell size maintenance in Anabaena . As a fundamental physical trait characteristic of each bacterial species, cell size control has been extensively studied in model bacteria such as E. coli and B. subtilis ( 38 ). However, such studies have been very scarce for cyanobacteria, despite the ecological importance of these organisms.…”

Section: Discussionmentioning

confidence: 99%

Control of Cell Size by c-di-GMP Requires a Two-Component Signaling System in the Cyanobacterium Anabaena sp. Strain PCC 7120

et al. 2023

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Section: Discussionmentioning

confidence: 99%

Control of Cell Size by c-di-GMP Requires a Two-Component Signaling System in the Cyanobacterium Anabaena sp. Strain PCC 7120

et al. 2023

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“…Molecular studies have identified pathways connecting nitrogen sensing to the cell-cycle machinery controlling the G2/M transition, resulting in rapid acceleration or inhibition of mitosis commitment upon sudden changes of the nitrogen source in the medium (Carlson et al, 1999;Petersen and Nurse, 2007;Chica et al, 2016). However, it has not yet been reported how different features of the cell geometry, such as volume, length, width or surface-to-volume ratio, are regulated across different types of growth rate modulation (Gu and Oliferenko, 2021;Kellogg and Levin, 2022).…”

Section: Descriptionmentioning

confidence: 99%

Fission yeast obeys a linear size law under nutrient titration

Bertaux

Kleijn

Marguerat

et al. 2023

Preprint

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Steady-state cell size and geometry depend on growth conditions. Here, we use an experimental setup based on continuous culture and single-cell imaging to study how cell volume, length, width and surface-to-volume ratio vary across a range of growth conditions including nitrogen and carbon titration, the choice of nitrogen source, and translation inhibition. Overall, we find cell geometry is not fully determined by growth rate and depends on the specific mode of growth rate modulation. However, under nitrogen and carbon titrations, we observe that the cell volume and the growth rate follow the same linear scaling.

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“…Most organisms control DNA replication based on environmental cues and nutrient conditions to ensure each daughter cell has a complete copy of the genome. Multiple mechanisms are used to coordinate DNA replication with other cellular processes, including metabolism and cell division (reviewed in Kellogg & Levin, 2022 ). Failure to properly regulate DNA replication can result in a variety of consequences, including cell division defects, anucleate daughter cells, DNA damage, and in higher organisms, disease (Magdalou et al, 2014 ; O'Donnell et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Multiple mechanisms for overcoming lethal over‐initiation of DNA replication

Anderson

Smith

Grossman

2022

Molecular Microbiology

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DNA replication is highly regulated and primarily controlled at the step of initiation. In bacteria, the replication initiator DnaA and the origin of replication oriC are the primary targets of regulation. Perturbations that increase or decrease replication initiation can cause a decrease in cell fitness. We found that multiple mechanisms, including an increase in replication elongation and a decrease in replication initiation, can compensate for lethal over‐initiation. We found that in Bacillus subtilis, under conditions of rapid growth, loss of yabA, a negative regulator of replication initiation, caused a synthetic lethal phenotype when combined with the dnaA1 mutation that also causes replication over‐initiation. We isolated several classes of suppressors that restored viability to dnaA1 ∆yabA double mutants. Some suppressors (relA, nrdR) stimulated replication elongation. Others (dnaC, cshA) caused a decrease in replication initiation. One class of suppressors decreased replication initiation in the dnaA1 ∆yabA mutant by causing a decrease in the amount of the replicative helicase, DnaC. We found that decreased levels of helicase in otherwise wild‐type cells were sufficient to decrease replication initiation during rapid growth, indicating that the replicative helicase is limiting for replication initiation. Our results highlight the multiple mechanisms cells use to regulate DNA replication.

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Nutrient availability as an arbiter of cell size

Cited by 20 publications

References 72 publications

Control of Cell Size by c-di-GMP Requires a Two-Component Signaling System in the Cyanobacterium Anabaena sp. Strain PCC 7120

Control of Cell Size by c-di-GMP Requires a Two-Component Signaling System in the Cyanobacterium Anabaena sp. Strain PCC 7120

Fission yeast obeys a linear size law under nutrient titration

Multiple mechanisms for overcoming lethal over‐initiation of DNA replication

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