Control of Cell Size by c-di-GMP Requires a Two-Component Signaling System in the Cyanobacterium
            <i>Anabaena</i>
            sp. Strain PCC 7120

Sun, Qin-Xue; Huang, Min; Zhang, Ju‐Yuan; Zeng, Xiaobo; Zhang, Cheng‐Cai

doi:10.1128/spectrum.04228-22

Cited by 7 publications

(15 citation statements)

References 47 publications

(80 reference statements)

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“…Our results also support the recent research that "cheaters" of QS were capable of active communication by recruiting organisms from other domains, forming a pair in which one organism carries the receptive gene and another the synthetic gene [16]. Additional QS communications in bacteria are intricately connected to AOA, such as the AI-2 system and cyclic-di-GMP system; the latter has been reported as a signal modulating the cell size of cyanobacteria [71,72]. The interplays observed between AOA metabolic pathways and bacterial quorum sensing underscores the essential role of communication in metabolic exchanges in the marine water column, which could enhance the growth efficiency of AOA and surrounding heterotrophic communities.…”

Section: Discussionsupporting

confidence: 88%

Metabolic activities of marine ammonia-oxidizing archaea orchestrated by quorum sensing

Pereira,

Qin,

Galand

et al. 2023

Preprint

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Ammonia-oxidizing archaea (AOA) play crucial roles in marine carbon and nitrogen cycles by fixing inorganic carbon and performing the initial step of nitrification. Evaluation of carbon and nitrogen metabolism popularly relies on functional genes such as amoA and accA. Increasing studies suggest that quorum sensing (QS) mainly studied in biofilms for bacteria may serve as a universal communication and regulation mechanism among prokaryotes; however, this has yet to be demonstrated in marine planktonic archaea. To bridge this knowledge gap, we employed a combination of metabolic activity markers (amoA, accA, and grs) to elucidate the regulation of AOA-mediated nitrogen and carbon processes, including their interactions with the surrounding heterotrophic population. Through co-transcription investigations linking metabolic markers to potential key QS genes, we discovered that QS molecules could regulate AOA's carbon, nitrogen, and lipid metabolisms under different conditions. Interestingly, specific AOA ecotypes showed a preference for employing distinct QS systems and a distinct QS circuit involving typical population. Overall, our data demonstrate that quorum sensing (QS) orchestrates nitrogen and carbon metabolism, including the exchange of organic metabolites between AOA and surrounding heterotrophic bacteria, which has been previously overlooked in marine AOA research.

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Section: Discussionsupporting

confidence: 88%

Metabolic activities of marine ammonia-oxidizing archaea orchestrated by quorum sensing

Pereira,

Qin,

Galand

et al. 2023

Preprint

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“…shown in Figure 3a, whatever the OD of the culture, cell elongation was observed under diazotrophic conditions, including the culture maintained at an OD 750 value of 1.0. With the same cell concentration (OD 750 = 1.0), cells of the WT became even smaller in length under diazotrophic conditions, as previously described (Neunuebel & Golden, 2008;Sun et al, 2023). Consistently, filaments of the mutant appeared yellowish in colour in comparison to those of the WT (Figure 3b), and further incubation under such conditions led to cell lysis.…”

Section: Hetf Inactivation Led To Defect Of Cell Division Under Diazo...supporting

confidence: 79%

HetF defines a transition point from commitment to morphogenesis during heterocyst differentiation in the cyanobacterium Anabaena sp. PCC 7120

Xing,

Liu,

Zhang

2023

Molecular Microbiology

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The filamentous cyanobacterium Anabaena sp. PCC 7120 is able to form heterocysts for nitrogen fixation. Heterocyst differentiation is initiated by combined‐nitrogen deprivation, followed by the commitment step during which the developmental process becomes irreversible. Mature heterocysts are terminally differentiated cells unable to divide, and cell division is required for heterocyst differentiation. Previously, we have shown that the HetF protease regulates cell division and heterocyst differentiation by cleaving PatU3, which is an inhibitor for both events. When hetF is required during the developmental program remains unknown. Here, by controlling the timing of hetF expression during heterocyst differentiation, we provide evidence that hetF is required just before the beginning of heterocyst morphogenesis. Consistent with this finding, transcriptome data show that most of the genes known to be involved in the early step (such as hetR and ntcA) or the commitment step (such as hetP and hetZ) of heterocyst development could be expressed in the ΔhetF mutant. In contrast, most of the genes involved in heterocyst morphogenesis and nitrogen fixation remain repressed in the mutant. These results indicated that in the absence of hetF, heterocyst differentiation is able to be initiated and proceeds to the stage just before heterocyst envelope formation.

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“…This multi-domain protein (CdgSH) harbours a GAF domain that does not contain the amino acids important for chromophore binding in a typical photosensory GAF domain. Further, it has been shown that a phosphodiesterase (CdgS), which is part of a two-component system including the multi-domain histidine kinase CdgK, controls the cell size of this cyanobacterium (Sun et al 2023 ). In both systems, the signal for the activation of c-di-GMP-related enzymes is unknown.…”

Section: Role Of Blue Light In Lifestyle Decisionsmentioning

confidence: 99%

Control of light-dependent behaviour in cyanobacteria by the second messenger cyclic di-GMP

2023

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Nucleotide-derived signalling molecules control a wide range of cellular processes in all organisms. The bacteria-specific cyclic dinucleotide c-di-GMP plays a crucial role in regulating motility-to-sessility transitions, cell cycle progression, and virulence. Cyanobacteria are phototrophic prokaryotes that perform oxygenic photosynthesis and are widespread microorganisms that colonise almost all habitats on Earth. In contrast to photosynthetic processes which are well understood, the behavioural responses of cyanobacteria have rarely been studied in detail. Analyses of cyanobacterial genomes have revealed that they encode a large number of proteins that are potentially involved in the synthesis and degradation of c-di-GMP. Recent studies have demonstrated that c-di-GMP coordinates many different aspects of the cyanobacterial lifestyle, mostly in a light-dependent manner. In this review, we focus on the current knowledge of light-regulated c-di-GMP signalling systems in cyanobacteria. Specifically, we highlight the progress made in understanding the most prominent behavioural responses of the model cyanobacterial strains Thermosynechococcus vulcanus and Synechocystis sp. PCC 6803. We discuss why and how cyanobacteria extract crucial information from their light environment to regulate ecophysiologically important cellular responses. Finally, we emphasise the questions that remain to be addressed.

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Control of Cell Size by c-di-GMP Requires a Two-Component Signaling System in the Cyanobacterium Anabaena sp. Strain PCC 7120

Cited by 7 publications

References 47 publications

Metabolic activities of marine ammonia-oxidizing archaea orchestrated by quorum sensing

Metabolic activities of marine ammonia-oxidizing archaea orchestrated by quorum sensing

HetF defines a transition point from commitment to morphogenesis during heterocyst differentiation in the cyanobacterium Anabaena sp. PCC 7120

Control of light-dependent behaviour in cyanobacteria by the second messenger cyclic di-GMP

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