Numerical and Chemotaxonomy of Polyphosphate Accumulating Acinetobacter Strains with High Polyphosphate: AMP Phosphotransferase (PPAT) Activity

Kämpfer, Peter; Bark, Kerstin; Busse, Hans‐Jürgen; Auling, Georg; Dott, Wolfgang

doi:10.1016/s0723-2020(11)80215-8

Cited by 48 publications

(32 citation statements)

References 46 publications

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“…In addition to the enzyme system based on polyphosphate : AMP phosphotransferase and adenylate kinase reported for Acinetobacter johnsonii [5,12,22], the polyphosphate glucokinase and glucose-6-phosphate-dependent NAD kinase in coryneform bacteria reported here are further enzymatic mechanisms by which organisms may benefit from polyphosphate as energy reserve. However, the role of this enzyme system in vivo still has to be investigated.…”

Section: Polyphosphate-dependent Enzyme Activitiesmentioning

confidence: 67%

“…Again, it should be pointed out that the overall process, often simply described as 'biological' phosphate removal from wastewater, is a complex combination of enzyme-mediated biological mechanisms (as reported here and elsewhere [1,4,12,22]), chemical mechanisms including phosphate adsorption at the outer membrane structures [4,20] of microorganisms and phosphate precipitation [1,2]. The exact 'biological' proportion is still to be investigated.…”

Section: Polyphosphate-dependent Enzyme Activitiesmentioning

confidence: 86%

“…Generally, the spectrum of polyphosphate-dependent enzymes in the coryneform organisms is very heterogeneous in comparison to the Gramnegative isolates studied previously [4,5,12,22]. A most interesting result was the detection of the polyphosphate glucokinase.…”

Section: Polyphosphate-dependent Enzyme Activitiesmentioning

confidence: 92%

“…Cellular fatty acids were transmethylated with methanolic HCI, and fatty acid methyl esters were analysed by gas liquid chromatography using a Shimadzu GC 9A equipped with a fused silica capillary column (50 m × 0.32 mm) as reported elsewhere [12]. Table 1 Specific activities of different polyphosphate-dependent enzymes and presence of polyphosphate fractions according to Clark et al [7] PGK, polyphosphate-dependent 3-phosphoglycerate kinase.…”

Section: Cellular Fatty Acidsmentioning

confidence: 99%

“…Although the enzymes polyphosphate : AMP phosphotransferase and adenylate kinase have been shown to form ATP from native and synthetic polyphosphate in strain Acinetobacterjohnsonii 210A [5,22] and several other strains belonging to the genus Acinetobacter [12], the role of this energy generating process within the overall process of 'biological' phosphate removal is far from clear. Several reports described the predominance of other genera than Acinetobacter in activated sludges showing enhanced phosphate removal [3,13,19,21].…”

Section: Introductionmentioning

confidence: 99%

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Polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge

Bark

Kämpfer

Sponner

et al. 1993

FEMS Microbiology Letters

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Eleven isolates obtained from a laboratory sewage treatment plant, most of them presumptively assigned to the coryneform genera Curtobacterium and Aureobacterium were studied for the presence of intracellular polyphosphates and polyphosphate dependent enzymes. All isolates stored polyphosphates and showed adenylate kinase activities ranging from 64 to 815 mU mg -1. Polyphosphate:AMP phosphotransferase could only be detected in one isolate. Three isolates showed a polyphosphate kinase activity also in minor amounts from 15 to 17 mU mg -1. A polyphosphate dependent NAD or 3-phosphoglycerate kinase could not be detected. Polyphosphate glucokinase activity was measured in cell-free extracts of nine isolates ranging from 2 to 376 mU mg-1. Three isolates showed in addition to the polyphosphate glucokinase, a glucose-6-phosphate-dependent NAD kinase. For the regeneration of NADP from NAD and polyphosphate, this enzyme system may give the isolates a distinct competitive advantage, especially for anabolic processes. The polyphosphate-dependent enzymes reported here may play an additional role in the complex process of 'biological' phosphate removal from wastewater.

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Section: Polyphosphate-dependent Enzyme Activitiesmentioning

confidence: 67%

Section: Polyphosphate-dependent Enzyme Activitiesmentioning

confidence: 86%

Section: Polyphosphate-dependent Enzyme Activitiesmentioning

confidence: 92%

Section: Cellular Fatty Acidsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge

Bark

Kämpfer

Sponner

et al. 1993

FEMS Microbiology Letters

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Diversity of PAH‐degrading bacteria in an airlift‐suspension reactor system for waste‐water cleaning

Maue

Dott

Kämpfer

1994

Acta Biotechnologica

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Different samples from a reactor system for the cleaning of an emulsified waste oil containing approximately 1 % of different polycyclic aromatic hydrocarbons (PAH) were studied for the diversity of phenanthrene, anthracene, and acenaphthene-metabolizing bacteria. From more than 28 different taxa found in the airlift-suspension reactor, nine were able to mineralize at least one of the PAH, belonging to the genera Bacillus, Mycobacterium, Nocardia, Sphingomonas, Alcaligenes, Pseudomonas, and the Flauobacrerinm/Cytohaga group. In the sludge from the thickener of the reactor system, a reduced number of metabolically active groups could be detected. The impact of differcnt precultivation substrates on the degradation capability was investigated with five PAH-degrading bacterial strains. Four isolates were influenced by the precultivation substrates, however, one isolate (Alcaligenes-like) as well as the mixture of the five strains showed no changes in their degradation capability. The results indicated a strong impact of the precultivation methods on the composition of bacterial communities and the activity and the degradation characteristics of bacteria with respect to different PAH.

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Studies on polyphosphate and poly‐β‐hydroxyalkanoate accumulation in Acinetobacter johnsonii 120 and some other bacteria from activated sludge in batch and continuous culture

Weltin

Hoffmeister

Dott

et al. 1996

Acta Biotechnologica

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Twelve bacterial isolates, four of them assigned to the genus Acinetobacter. were taken from sewage of a treatment plant with Enhanced Biological Phosphorus Removal (EBPR) and screened for phosphorus uptake, polyphosphate (polyp) accumulation and adsorption under limited carbon and nitrogen conditions. In addition, poly-J-hydroxyalkanoate (PHA) production was studied under carbon. nitrogen, phosphorus, and oxygen limitation. Under C limitation, the uptake of phosphorus was highest, ranging up to 66 mg P per g dry weight (dw) for the Acinetobacter isolates, whereas the highest amount of polyp was detected under limited N conditions (up to 25 mg PolyPIg dw). Extra-cellular polyp was detected, however to a minor extent, accounting for a maximum of 10% of the total polyp in one Acinetobacter isolate. The highest PHA concentration (given as 3-hydroxybutyrate, 3 HB) with 211 mg 3 H B / g dry weight (21% of the dried cell mass) was found for A. johnsonii 120 under nitrogen limitation. but also under P and 9 limitation, PHA, mainly poly-$3-hydroxybutyrate and poly-P-hydroxyvaler, were produced. Three isolates, assigned to the genus Pseudomonas, showed even higher values (345427 mg 3 HB / g dw) under N limitation. Studies with Acinetobacter johnsonii 120 in continuous culture, simulating the aerobic/anaerobic periods of a waste-water matment plant, resulted in a P elimination of 36% at an anaerobic contact time of 0.6 h. This value increased to 51% at an anaerobic contact time of 3.1 h. No release of phosphate and no uptake of acetate could be detected during the anaerobic period. In addition, Acinetobacter johnsonii 120 was not able to synthesize PHA under anaerobic conditions. By changing the anaerobic conditions to aerobic, a continuous decrease of the polyp content relative to the totalp content from 45% (day 1 of the aerobic process) to 19% (day 17 of the aerobic process) was observed. The amount of PHA increased to 50.4 mg 3 HB / g dw under aerobic conditions. The results indicate again that the EPBR process cannot be defined by simply applying the knowledge of the metabolic processes, observed or assumed in Acinetobacter pure cultures, to the complexity of the process in sewage treatment plants.

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Numerical and Chemotaxonomy of Polyphosphate Accumulating Acinetobacter Strains with High Polyphosphate: AMP Phosphotransferase (PPAT) Activity

Cited by 48 publications

References 46 publications

Polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge

Polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge

Diversity of PAH‐degrading bacteria in an airlift‐suspension reactor system for waste‐water cleaning

Studies on polyphosphate and poly‐β‐hydroxyalkanoate accumulation in Acinetobacter johnsonii 120 and some other bacteria from activated sludge in batch and continuous culture

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