Studies on polyphosphate and poly‐β‐hydroxyalkanoate accumulation in <i>Acinetobacter johnsonii</i> 120 and some other bacteria from activated sludge in batch and continuous culture

Weltin, D.; Hoffmeister, Diego; Dott, Wolfgang; Kämpfer, Peter

doi:10.1002/abio.370160202

Cited by 11 publications

(7 citation statements)

References 20 publications

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“…This can be explained by high RNA content in growing cells (25) and by the fact that RNA can be used as a P reserve when the cells become P limited (18). Since bacteria can store P as polyphosphate (32), one can expect lower C:P and N:P ratios when elements other than P are limiting the growth and P is in excess, which was also the case in our experiments. However, this storage capacity of P was not so large that it could cover the P losses due to a reduction in RNA content that probably occurred during growth limitation.…”

Section: Discussionmentioning

confidence: 60%

“…RNA is usually the largest P pool of the cell, and the growth rate will therefore affect the P content of the cell. However, P can also be stored as polyphosphate during growth limitation and thereby affect the P content of the cell (32). Other compounds, such as carbohydrates, can also be stored during growth limitation (31) and therefore affect the stoichiometry of the cell.…”

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confidence: 99%

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Elemental Composition (C, N, P) and Cell Volume of Exponentially Growing and Nutrient-Limited Bacterioplankton

Vrede

Heldal

Norland

et al. 2002

Appl Environ Microbiol

301

220

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Marine bacterioplankton were isolated and grown in batch cultures until their growth became limited by organic carbon (C), nitrogen (N), or phosphorus (P). Samples were taken from the cultures at both the exponential and stationary phases. The elemental composition of individual bacterial cells was analyzed by X-ray microanalysis with an electron microscope. The cell size was also measured. The elemental content was highest in exponentially growing cells (149 ؎ 8 fg of C cell ؊1 , 35 ؎ 2 fg of N cell ؊1 , and 12 ؎ 1 fg of P cell ؊1 ; average of all isolates ؎ standard error). The lowest C content was found in C-limited cells (39 ؎ 3 fg of C cell ؊1 ), the lowest N content in C-and P-limited cells (12 ؎ 1 and 12 ؎ 2 fg of N cell ؊1 , respectively), and the lowest P content in P-limited cells (2.3 ؎ 0.6 fg of P cell ؊1 ). The atomic C:N ratios varied among treatments between 3.8 ؎ 0.1 and 9.5 ؎ 1.0 (average ؎ standard error), the C:P ratios between 35 ؎ 2 and 178 ؎ 28, and the N:P ratios between 6.7 ؎ 0.3 and 18 ؎ 3. The carbon-volume ratios showed large variation among isolates due to different types of nutrient limitation (from 51؎ 4 to 241 ؎ 38 fg of C m ؊1 ; average of individual isolates and treatments ؎ standard error). The results show that different growth conditions and differences in the bacterial community may explain some of the variability of previously reported elemental and carbonvolume ratios.

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Section: Discussionmentioning

confidence: 60%

mentioning

confidence: 99%

Elemental Composition (C, N, P) and Cell Volume of Exponentially Growing and Nutrient-Limited Bacterioplankton

Vrede

Heldal

Norland

et al. 2002

Appl Environ Microbiol

301

220

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“…However, in our study, we kept the growth rate constant at 0.41 d -1 , and thus, we can exclude the possibility that the observed change in the bacterial cellular P content was a growth rate-related effect. In some bacteria, P can also be stored as polyphosphate during growth limitation (Weltin et al 1996), and microscopic observations of bacterial cells in the P50 treatment did indeed reveal the presence of such polyphosphate granules (data not shown). The bacterial molar C:P ratio fluctuated 211 (Table 2).…”

Section: Cell Characteristicsmentioning

confidence: 99%

Effects of phosphorus loading on interactions of algae and bacteria: reinvestigation of the phytoplankton-bacteria paradox in a continuous cultivation system

Mindl¹,

Sonntag

Pernthaler

et al. 2005

Aquat. Microb. Ecol.

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The effect of different phosphorus loads (L P ) on the phosphorus (P) and carbon (C) content (biomass) of algae and bacteria was assessed in continuous culture. We tested if a mixed freshwater microbial assemblage co-cultured with a phytoflagellate (Cryptomonas phaseolus) would comply with the 'phytoplankton-bacteria paradox ' (sensu Bratbak & Thingstad 1985). This hypothesis states that the ratio of bacterial to algal abundance changes to the benefit of bacteria with decreasing L P . However, the phenomenon was originally investigated by simultaneously altering L P and microbial growth rates, and it is unclear to which extent it can be assigned to either parameter. Therefore, we set up 3 chemostat systems in triplicate at equal dilution rates, but with daily L P of 21, 41 or 62 µg l -1 d -1 (corresponding to 50, 100 and 150 µg P l -1 ). Higher L P led to a 5-fold increase in total algal abundance and biomass but to less than a doubling of these parameters in the bacterial assemblage. Total biomass ratios of bacteria to algae changed from 0.18 to 0.06 with increasing L P , while the bacteria-algae total phosphorus ratios decreased from 0.80 to 0.17. The cellular C:P ratio of algae remained similar at all P concentrations, whereas the molar C:P ratios of bacterial cells significantly increased at higher L P (from 44 to 73). An enrichment experiment with the 50 µg P l -1 treatment demonstrated that bacteria at the lowest L P were co-limited by P and C, and that increased P stimulated mainly the algal fraction. The phytoplankton-bacteria paradox at the level of a mixed microbial assemblage is thus characterised by the following aspects: (1) bacteria profit from their high affinity to P and are better competitors at lower L P ; (2) although algae compete with bacteria for P, P-limited algae release extracellular C that stimulates growth of their bacterial competitors; (3) when bacteria depend on algae as their sole source of organic C, this provides a feedback mechanism by which algae limit the abundance of their competitors at higher L P ; (4) large oscillations in the bacteria-algae ratios at the lowest L P point to a greater instability of this interaction with stronger P competition. However, bacteria were not able to outcompete C. phaseolus, as algae were their only C source.KEY WORDS: Algae-bacteria interaction · Chemostat · Continuous cultivation system · Cryptomonas phaseolus · Eutrophication · Phosphorus · Phytoplankton-bacteria paradox Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 38: [203][204][205][206][207][208][209][210][211][212][213] 2005 nutrients, such as nitrogen or phosphorus, the relationship between bacteria and algae is not considered mutualistic, but competitive (Currie & Kalff 1984b, Elser et al. 1995, Vadstein 2000.The competition for P between bacteria and algae cannot easily be studied in situ, i.e. in a planktonic food web of high complexity. Continuous cultivation systems have the advantage that algae and bacteria experience...

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“…and Acinetobacter spp. have been reported to be able to produce PHA [9,42,44,48]. In addition, Acinetobacter spp.…”

Section: Microbial Community Analysismentioning

confidence: 99%

polyhydroxyalkanoate production from different carbon substrates using sludge from a wastewater treatment plant: Microbial communities, polymer compositions, and thermal characteristics

Woraittinun

Suwannasilp

2017

Env Prog and Sustain Energy

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Polyhydroxyalkanoate (PHA) is a microbial storage biopolymer that can be used in biodegradable plastics. This study investigates the effects of two carbon substrates (acetate and propionate) on PHA production using sludge from a wastewater treatment plant in terms of their microbial communities, PHA contents, polymer compositions and structures, and thermal characteristics of the PHA. Sludge was enriched aerobically with acetate or propionate in sequencing batch reactors (SBRs) under feast/famine feeding conditions. The sludge enriched with acetate produced a homopolymer of hydroxybutyrate (HB) and accumulated a maximum PHA of 44.78% gPHA/g mixed liquor suspended solids (MLSS). In contrast, the sludge enriched with propionate produced a copolymer of HB and hydroxyvalerate (HV) and accumulated a maximum PHA of 12.65% gPHA/gMLSS. The results from the microbial community analysis using 16S rRNA gene amplicon sequencing showed that bacteria belonging to the phylum Bacteroidetes and class Alphaproteobacteria appeared to be dominant in the system fed with acetate when PHA content was at its maximum. In contrast, Alphaproteobacteria and Gammaproteobacteria were dominant in the system fed with propionate when the PHA content was at its maximum. The PHA produced from acetate also had a higher melting temperature, higher glass transition temperature, and higher crystallinity than did that produced from propionate. © 2017 American Institute of Chemical Engineers Environ Prog, 36: 1754–1764, 2017

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Studies on polyphosphate and poly‐β‐hydroxyalkanoate accumulation in Acinetobacter johnsonii 120 and some other bacteria from activated sludge in batch and continuous culture

Cited by 11 publications

References 20 publications

Elemental Composition (C, N, P) and Cell Volume of Exponentially Growing and Nutrient-Limited Bacterioplankton

Elemental Composition (C, N, P) and Cell Volume of Exponentially Growing and Nutrient-Limited Bacterioplankton

Effects of phosphorus loading on interactions of algae and bacteria: reinvestigation of the phytoplankton-bacteria paradox in a continuous cultivation system

polyhydroxyalkanoate production from different carbon substrates using sludge from a wastewater treatment plant: Microbial communities, polymer compositions, and thermal characteristics

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Studies on polyphosphate and poly‐β‐hydroxyalkanoate accumulation in Acinetobacter johnsonii 120 and some other bacteria from activated sludge in batch and continuous culture

Cited by 11 publications

References 20 publications

Elemental Composition (C, N, P) and Cell Volume of Exponentially Growing and Nutrient-Limited Bacterioplankton

Elemental Composition (C, N, P) and Cell Volume of Exponentially Growing and Nutrient-Limited Bacterioplankton

Effects of phosphorus loading on interactions of algae and bacteria: reinvestigation of the phytoplankton-bacteria paradox in a continuous cultivation system

polyhydroxyalkanoate production from different carbon substrates using sludge from a wastewater treatment plant: Microbial communities, polymer compositions, and thermal characteristics

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Product

Resources

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Effects of phosphorus loading on interactions of algae and bacteria: reinvestigation of the phytoplankton-bacteria paradox in a continuous cultivation system