Null Association between Vitamin D and PSA Levels among Black Men in a Vitamin D Supplementation Trial

Chandler, Paulette D.; Giovannucci, Edward; Scott, Jerry B.; Bennett, Gary G.; Ng, Kimmie; Chan, Andrew T.; Hollis, Bruce W.; Emmons, Karen M.; Fuchs, Charles S.; Drake, Bettina F.

doi:10.1158/1055-9965.epi-14-0522

Cited by 23 publications

(21 citation statements)

References 14 publications

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“…In another study conducted to study the association between VD and PSA levels among black men, it was found that there was no effect on PSA levels, free or total with VD supplementation in men without a cancer diagnosis [22]. However, no correlation was made between patients who had cancer and VD deficiency.…”

Section: Discussionmentioning

confidence: 99%

Association of Vitamin D With Prostate Carcinoma: A Single Institutional Observational Study

Choubey¹,

Pipara²,

Mittal³

2017

World J Nephrol Urol

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Background: Recent studies highlight a role for vitamin D (VD) in the growth and differentiation of various cell types. The biologically active form of vitamin D3 is 1,25-dihydroxyvitamin D3. Most cells of the body including prostate cells have vitamin D receptor (VDR) and VD metabolizing enzymes, and can respond to 1,25-VD. Literature supports multipronged effects of 1,25-VD in the prevention of prostate carcinoma development and progression. However, the relationship between prostate carcinoma and VD is still not entirely understood. There are no studies conducted on the association of VD and prostate carcinoma among the Asian population and our study is the first of its kind in literature and hence the need for the same.

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Section: Discussionmentioning

confidence: 99%

Association of Vitamin D With Prostate Carcinoma: A Single Institutional Observational Study

Choubey¹,

Pipara²,

Mittal³

2017

World J Nephrol Urol

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“…Kristal et al recently reported reduced risk of Gleason 7-10 prostate cancer among AAs in the upper three quintiles of serum 25(OH)D compared to the lower two quintiles in the Selenium and Vitamin E Cancer Prevention Trial [22]. In a recent randomized, placebo-controlled clinical trial, varying doses of vitamin D supplementation had no effect on PSA levels in healthy AA men without prostate cancer [50]. The current study results, along with these recent reports underscore the need for more research on vitamin D and prostate health among AAs who have been underrepresented in previous research studies.…”

Section: Discussionmentioning

confidence: 99%

Vitamin D and Related Genes, Race, and Prostate Cancer Aggressiveness

Steck¹

2014

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“…A neighboring unbleached cell served as a control for focus drift and photobleaching during image acquisition. Normalization was done to the pre-bleach data point (39). At least 10 cells were collected per treatment condition.…”

Section: Live Cell Imaging Using Flip Microscopymentioning

confidence: 99%

Phosphorylation of Human Retinoid X Receptor α at Serine 260 Impairs Its Subcellular Localization, Receptor Interaction, Nuclear Mobility, and 1α,25-Dihydroxyvitamin D3-dependent DNA Binding in Ras-transformed Keratinocytes

Jusu

Presley

Kremer

2017

Journal of Biological Chemistry

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Human retinoid X receptor α (hRXRα) plays a critical role in DNA binding and transcriptional activity through heterodimeric association with several members of the nuclear receptor superfamily, including the human vitamin D receptor (hVDR). We previously showed that hRXRα phosphorylation at serine 260 through the Ras-Raf-MAPK ERK1/2 activation is responsible for resistance to the growth inhibitory effects of 1α,25-dihydroxyvitamin D (1α,25(OH)D), the biologically active metabolite of vitamin D To further investigate the mechanism of this resistance, we studied intranuclear dynamics of hVDR and hRXRα-tagged constructs in living cells together with endogenous and tagged protein in fixed cells. We find that hVDR-, hRXRα-, and hVDR-hRXRα complex accumulate in the nucleus in 1α,25(OH)D-treated HPK1A cells but to a lesser extent in HPK1ARas-treated cells. Also, by using fluorescence resonance energy transfer (FRET), we demonstrate increased interaction of the hVDR-hRXRα complex in 1α,25(OH)D-treated HPK1A but not HPK1ARas cells. In HPK1ARas cells, 1α,25(OH)D-induced nuclear localization and interaction of hRXRα are restored when cells are treated with the MEK1/2 inhibitor UO126 or following transfection of the non-phosphorylatable hRXRα Ala-260 mutant. Finally, we demonstrate using fluorescence loss in photobleaching and quantitative co-localization with chromatin that RXR immobilization and co-localization with chromatin are significantly increased in 1α,25(OH)D-treated HPK1ARas cells transfected with the non-phosphorylatable hRXRα Ala-260 mutant. This suggests that hRXRα phosphorylation significantly disrupts its nuclear localization, interaction with VDR, intra-nuclear trafficking, and binding to chromatin of the hVDR-hRXR complex.

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Null Association between Vitamin D and PSA Levels among Black Men in a Vitamin D Supplementation Trial

Cited by 23 publications

References 14 publications

Association of Vitamin D With Prostate Carcinoma: A Single Institutional Observational Study

Association of Vitamin D With Prostate Carcinoma: A Single Institutional Observational Study

Vitamin D and Related Genes, Race, and Prostate Cancer Aggressiveness

Phosphorylation of Human Retinoid X Receptor α at Serine 260 Impairs Its Subcellular Localization, Receptor Interaction, Nuclear Mobility, and 1α,25-Dihydroxyvitamin D3-dependent DNA Binding in Ras-transformed Keratinocytes

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