Nucleus- and plastid-targeted annexin 5 promotes reproductive development in Arabidopsis and is essential for pollen and embryo formation

Lichocka, Małgorzata; Rymaszewski, Wojciech; Morgiewicz, Karolina; Barymow-Filoniuk, Izabela; Chlebowski, Aleksander; Sobczak, Mirosław; Samuel, Marcus A.; Schmelzer, Elmon; Krzymowska, Magdalena; Hennig, Jacek

doi:10.1101/331249

Cited by 3 publications

(8 citation statements)

References 51 publications

(58 reference statements)

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“…Annexin genes have been reported to play significant roles in plant growth, development, and overcoming abiotic adversity [27][28][29][30][31][32][33][34][35][36][37]. Numerous annexin family gene members have been identified in other plants.…”

Section: Discussionmentioning

confidence: 99%

Identification, Characterization and Expression Analysis Reveal the Potential Function of Annexin Genes in Response to Abiotic stress, Calcium and Hormones in Cassava (Manihot esculeta Crantz)

Li¹,

Wang²,

Zhou³

et al. 2019

Preprint

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Background The calcium (Ca2+)-dependent phospholipid binding protein annexin gene family, which is known to be related to membrane lipid and cytoskeletal components, is involved in a diverse range of biological functions. However, in cassava (Manihot esculenta Crantz), no studies focusing on the roles of annexin genes in response to abiotic stresses, calcium, and hormones have been informed. Results 12 annexin genes were found and assigned to eight chromosomes in the cassava genome. All of the MeAnns contain a typical annexin domain with four 70-amino acid repeats. The MeAnns are classified into six groups in the phylogenetic tree. In their promoter regions, MeAnns possess at least 3 hormone response-related cis-elements and 1 abiotic stress response-related cis-acting element. MeAnn1, MeAnn2 and MeAnn5 exhibit very high levels of expression in each tested organs or tissues. By contrast, MeAnn12 exhibits very low levels in all the tested organs or tissues. qRT-PCR analysis indicates that both MeAnn5 and MeAnn9 have significantly high expression in leaves after cold, drought, and salt treatments and are highly responsive to CaCl2, GA and JA treatments. MeAnn2 and MeAnn10 are significantly downregulated in leaves by cold, drought and salt stress and negatively respond to CaCl2, GA and JA. The expression patterns of MeAnns under cold, drought, and salt stress are irregular in shoots. In roots, MeAnn1 and MeAnn9 are downregulated by cold, CaCl2 and JA treatments, while their other gene expression patterns are irregular. Conclutions In this study, we identified annexin genes in cassava and our expression profiling analysis demonstrated that cassava annexin genes responded to multiple stresses. Our results laid the foundation for further study of the function of cassava anesxin genes and provided an entry point for understanding the response mechanism of cassava to abiotic stress.

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Section: Discussionmentioning

confidence: 99%

Identification, Characterization and Expression Analysis Reveal the Potential Function of Annexin Genes in Response to Abiotic stress, Calcium and Hormones in Cassava (Manihot esculeta Crantz)

Li¹,

Wang²,

Zhou³

et al. 2019

Preprint

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“…pLAT52:ANN5-YFP and pLAT52:ANN1-YFP transformants were generated in Arabidopsis Col-0 background. ANN5 RNAi-silenced lines 13 and 15 were previously generated in our lab (Lichocka et al, 2018). Arabidopsis plants were grown in Jiffy7 pots in controlled-environment chambers (Percival Scientific, Iowa, USA) at 22°C, ca.…”

Section: Methodsmentioning

confidence: 99%

“…In contrast to ANN1 , which is constitutively expressed in vegetative tissues and also during pollen development, the onset of ANN5 expression occurs in the bicellular microspore and its transcripts accumulate until the pollen maturity (Honys and Twell, 2004; Zhu et al, 2014b). Previous studies showed that downregulation of ANN5 level compromised pollen viability and vigor reflected by collapsing of the pollen grains and formation of the smaller grains with a reduced competitiveness compared to the wild-type grains (Zhu et al, 2014b; Lichocka et al, 2018). It was also shown that ANN5 in vitro binds to liposomes composed of phosphatidyl cholines and phosphatidyl serines in a calcium-dependent manner (Zhu et al, 2014a).…”

Section: Introductionmentioning

confidence: 98%

“…In Arabidopsis ANN5 is predominately expressed in developing pollen grains and its essential for male gametophyte development (Zhu et al, 2014b; Lichocka et al, 2018). Interestingly, the homologous genes in B. napus , are mainly expressed in buds and newly formed pistils which might indicate species-specific function of the homologous annexins (He et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Arabidopsis annexin 5 controls plasma membrane properties in mature pollen grains

Lichocka

Krzymowska

Górecka

et al. 2021

Preprint

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In Arabidopsis, a dry stigma surface enables a gradual hydration of pollen grains by a controlled release of water. Occasionally the grains may be exposed to extreme precipitations that cause rapid water influx, swelling and eventually lead to pollen membrane (PM) rupture. In metazoans, calcium- and phospholipid-binding proteins, referred to as annexins participate in repair of the plasma membrane damages. It remains unclear, however, how this process is conducted in plants. Here, we examined whether the plant annexin 5 (ANN5), the most abundant member of the annexin family in pollen, is involved in the restoration of PM integrity. We analyzed a cellular dynamics of ANN5 in the pollen grains undergoing in vitro and in vivo hydration. We observed a transient ANN5 association to PM during the in vitro hydration that did not occur in the pollen grains being hydrated on the stigma. To simulate a rainfall, we performed spraying of the pollinated stigma with deionized water that induced ANN5 accumulation at PM. Similarly, calcium or magnesium application affected PM properties and induced ANN5 recruitment to PM. Our data suggest a model, in which ANN5 is involved in the maintenance of membrane integrity in pollen grains exposed to osmotic or ionic imbalances.

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“…In Arabidopsis thaliana (Arabidopsis), the nuclear gene RAB GTPASE HOMOLOG 8D (RAB8D, also called RABE1B) encodes a plastid-targeted counterpart of the prokaryotic translation elongation factor thermal unstable (EF-Tu), and is constitutively expressed in both shoots and roots (Lichocka et al, 2018;Liu et al, 2019). In prokaryotes, EF-Tu functions in translation through transporting aminoacyl-tRNA to the A site of the 70S ribosome.…”

Section: Introductionmentioning

confidence: 99%

RAB GTPASE HOMOLOG 8D is required for the maintenance of both the root stem cell niche and the meristem

Sun

et al. 2021

The Plant Journal

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Previous studies have suggested that the plastid translation elongation factor, elongation factor thermo unstable (EF-Tu), encoded by RAB GTPASE HOMOLOG 8D (RAB8D) is essential for plant growth. Here, through analyzing the root phenotypes of two knock-down alleles of RAB8D (rab8d-1 and rab8d-2), we further revealed a vital role for RAB8D in primary root development through the maintenance of both the stem cell niche (SCN) and the meristem. Our results showed that RAB8D deficiency affects the root auxin response and SCN maintenance signaling. RAB8D interacts with GENOMES UNCOUPLED 1 (GUN1) in vivo. Further analysis revealed that GUN1 is over-accumulated and is required for both stem cell death and maintenance of root architecture in rab8d Arabidopsis mutants. The ATAXIA-TELANGIECTASIA-MUTATED (ATM)-SUPPRESSOR OF GAMMA RESPONSE 1 pathway is involved in the regulation of root meristem size through upregulating SIAMESE-RELATED 5 expression in the rab8d-2 allele. Moreover, ETHYLENE RESPONSE FACTOR 115 is highly expressed in rab8d-2, which plays a role in further quiescent center division. Our observations not only characterized the role of RAB8D in root development, but also uncovered functions of GUN1 and ATM in response to plastid EF-Tu deficiency.

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Nucleus- and plastid-targeted annexin 5 promotes reproductive development in Arabidopsis and is essential for pollen and embryo formation

Abstract: 4 8 Background 4 9 7 2of ANN5 is to orchestrate the plastidial and nuclear genome activities via protein-7 3 protein interactions however not only in maturing pollen but also during the transition 7 4 from the vegetative to the generative growth and embryo development. 7 5 7 6

Cited by 3 publications

References 51 publications

Identification, Characterization and Expression Analysis Reveal the Potential Function of Annexin Genes in Response to Abiotic stress, Calcium and Hormones in Cassava (Manihot esculeta Crantz)

Identification, Characterization and Expression Analysis Reveal the Potential Function of Annexin Genes in Response to Abiotic stress, Calcium and Hormones in Cassava (Manihot esculeta Crantz)

Arabidopsis annexin 5 controls plasma membrane properties in mature pollen grains

RAB GTPASE HOMOLOG 8D is required for the maintenance of both the root stem cell niche and the meristem

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