Nucleotide sequence of the coat protein coding region of the potyvirus tobacco vein-banding mosaic virus

Chang, Ban‐Yang; Huang, Ching-Wen; Yeh, Shyi-Dong; Chiang, J. K.; Hung, Li-Sang; Hu, Heyu

doi:10.1007/bf01310035

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Tomato cultivation in Taiwan suffers from numerous insect-borne viruses, including potato virus Y (PVY) [3], chilli veinal mottle virus (ChiVMV) [4], pepper veinal mottle virus (PVMV) [5], pepper mottle virus (PepMoV) [6], and tobacco vein banding mosaic virus (TVBMV) [7] of the aphid-transmitted genus Potyvirus in the family Potyviridae. Viruses belonging to the genus Potyvirus have a monopartite, single-stranded positive-sense RNA genome of about 10 kb, encapsidated by multiple units of a single type of coat protein (CP) in flexuous and filamentous particles of 720-850 nm long and 12-15 nm in diameter.…”

Section: Introductionmentioning

confidence: 99%

Development of Attenuated Viruses for Effective Protection against Pepper Veinal Mottle Virus in Tomato Crops

Wang,

Lin,

Chen

2024

Viruses

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Tomato (Solanum lycopersicum) is the most important vegetable and fruit crop in the family Solanaceae worldwide. Numerous pests and pathogens, especially viruses, severely affect tomato production, causing immeasurable market losses. In Taiwan, the cultivation of tomato crops is mainly threatened by insect-borne viruses, among which pepper veinal mottle virus (PVMV) is one of the most prevalent. PVMV is a member of the genus Potyvirus of the family Potyviridae and is non-persistently transmitted by aphids. Its infection significantly reduces tomato fruit yield and quality. So far, no PVMV-resistant tomato lines are available. In this study, we performed nitrite-induced mutagenesis of the PVMV tomato isolate Tn to generate attenuated PVMV mutants. PVMV Tn causes necrotic lesions in Chenopodium quinoa leaves and severe mosaic and wilting in Nicotiana benthamiana plants. After nitrite treatment, three attenuated PVMV mutants, m4-8, m10-1, and m10-11, were selected while inducing milder responses to C. quinoa and N. benthamiana with lower accumulation in tomato plants. In greenhouse tests, the three mutants showed different degrees of cross-protection against wild-type PVMV Tn. m4-8 showed the highest protective efficacy against PVMV Tn in N. benthamiana and tomato plants, 100% and 97.9%, respectively. A whole-genome sequence comparison of PVMV Tn and m4-8 revealed that 20 nucleotide substitutions occurred in the m4-8 genome, resulting in 18 amino acid changes. Our results suggest that m4-8 has excellent potential to protect tomato crops from PVMV. The application of m4-8 in protecting other Solanaceae crops, such as peppers, will be studied in the future.

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Section: Introductionmentioning

confidence: 99%

Development of Attenuated Viruses for Effective Protection against Pepper Veinal Mottle Virus in Tomato Crops

Wang,

Lin,

Chen

2024

Viruses

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“…Tobacco vein banding mosaic virus (TVBMV) was first discovered in Taiwan in 1964 [1] and confirmed to be a distinct species of the largest plant virus genus, Potyvirus, in 1994 [2,3]. TVBMV was once a major threat to tobacco production in some regions including Taiwan and northern USA, but only occurred rarely in most parts of China [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

The complete genomic sequence of Tobacco vein banding mosaic virus and its similarities with other potyviruses

Lan

Wang

et al. 2007

Virus Genes

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The complete genomic sequence of an isolate of Tobacco vein banding mosaic virus (TVBMV-YND) from Yunnan, China was determined by sequencing overlapping cDNA fragments obtained by RT-PCR with degenerate and/or specific primers. The genome is composed of 9,570 nucleotides (nt) excluding the 3'-terminal poly (A) tail and contains one single open reading frame of 9,240 nt encoding a large polyprotein of 3,079 amino acids with predicted Mr of 348.6 kDa. Phylogenetic analysis of complete genomic sequences confirmed that TVBMV is a distinct species of the genus Potyvirus. Different parts of TVBMV-YND genome shared different levels of identity with other species of potyviruses, while most parts showed greatest identity with Chilli veinal mottle virus among the potyviruses with available complete genomic sequences. TVBMV-YND had a rare Q/N cleavage site for NIb/CP and uncommon RITC motif in HC-Pro that is crucial for aphid transmission of potyviruses.

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“…TVBMV, which was discovered in Taiwan in 1964 (Chin, 1966;Tian et al, 2007), is a distinct member of the genus Potyvirus (Chang et al, 1994;Habera et al, 1994). Th e genome of TVBMV is a single-stranded positive-sense RNA with 9570 nucleotides (nt).…”

Section: Introductionmentioning

confidence: 99%

Characterization of 10 tobacco vein banding mosaic virus isolates from China

Yuan¹,

Song²,

Li³

et al. 2012

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Summary. -Th e complete RNA genome sequences of 10 tobacco vein banding mosaic virus (TVBMV) isolates from China were determined using fi ve overlapping cDNA clones. TVBMV was divided into three groups for HC-Pro and CP and into two groups for P3 and 6K1. With more isolate sequences analyzed, the phylogenetic results suggest that TVBMV could be divided into four subgroups based on HC-Pro and CP and into three subgroups with P3 and 6K1. Nucleotide sequence diversity analysis showed geographical diff erentiation among the TVBMV isolates. Th ree of the 10 isolates were found to have undergone recombination and new recombination sites were identifi ed in the TVBMV genome. All coding genes were under negative selection and the population appeared to have remained stable over a long period. Th is study also provides preliminary data on the 3´-untranslated region as potentially the best genome sequence for developing transgenic tobacco.

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Nucleotide sequence of the coat protein coding region of the potyvirus tobacco vein-banding mosaic virus

Cited by 6 publications

References 25 publications

Development of Attenuated Viruses for Effective Protection against Pepper Veinal Mottle Virus in Tomato Crops

Development of Attenuated Viruses for Effective Protection against Pepper Veinal Mottle Virus in Tomato Crops

The complete genomic sequence of Tobacco vein banding mosaic virus and its similarities with other potyviruses

Characterization of 10 tobacco vein banding mosaic virus isolates from China

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