The complete genomic sequence of Tobacco vein banding mosaic virus and its similarities with other potyviruses

Abstract

The complete genomic sequence of an isolate of Tobacco vein banding mosaic virus (TVBMV-YND) from Yunnan, China was determined by sequencing overlapping cDNA fragments obtained by RT-PCR with degenerate and/or specific primers. The genome is composed of 9,570 nucleotides (nt) excluding the 3'-terminal poly (A) tail and contains one single open reading frame of 9,240 nt encoding a large polyprotein of 3,079 amino acids with predicted Mr of 348.6 kDa. Phylogenetic analysis of complete genomic sequences confirmed that TVBMV is a distinct species of the genus Potyvirus. Different parts of TVBMV-YND genome shared different levels of identity with other species of potyviruses, while most parts showed greatest identity with Chilli veinal mottle virus among the potyviruses with available complete genomic sequences. TVBMV-YND had a rare Q/N cleavage site for NIb/CP and uncommon RITC motif in HC-Pro that is crucial for aphid transmission of potyviruses.

DEVELOPMENTALLY REGULATED PLASMA MEMBRANE PROTEIN of Nicotiana benthamiana Contributes to Potyvirus Movement and Transports to Plasmodesmata via the Early Secretory Pathway and the Actomyosin System

“…Tobacco vein banding mosaic virus (TVBMV) is a distinct potyvirus mainly infecting solanaceous crops (Tian et al, 2007;Yu et al, 2007;Zhang et al, 2011). In this article, we provide evidence showing the length requirements of the PIPO domains for its function in mediating TVBMV movement and the restoration of the movement-defective TVBMV mutants.…”

Characterization of 10 tobacco vein banding mosaic virus isolates from China

“…Total RNA was extracted from 100-mg leaf tissues using Trizol reagent and cDNA was obtained with a RevertAid First Strand cDNA Synthesis Kit following the manufacturer΄s instructions (MBI Fermentas, Germany). Five pairs of degenerate primers (Table1) were designed according to previously reported full-length genome sequences of TVBMV-YND and TVBMV-HN39 (Wang et al, 2010;Yu et al, 2007) to construct the entire genome sequences of the 10 TVBMV isolates. Th e 5ʹ-end of the RNA genome was amplifi ed by 5ʹ-RACE using a 5ʹ-Full RACE Kit (Takara, China).…”

“…TVBMV was once a major threat to tobacco production activities in North America and Taiwan, but it has rarely occurred in most regions of China (Reddick et al, 1992;Tian et al, 2007;Yu et al, 2007). Due to the lack of TVBMVresistant tobacco (Zhang et al, 2011), TVBMV may potentially spread quickly and cause serious damages to tobacco production in commercial planting areas.…”

“…Specifi cally, the HC-Pro, P3, 6K1, and CP genes have been only moderately studied (Tian et al, 2007;Zhang et al, 2011). Complete genomes of only two isolates have been reported (Wang et al, 2010;Yu et al, 2007). Th e TVBMV isolates could be divided into three and two groups based on the analysis of HC-Pro and CP and on that of P3 and 6K1, respectively.…”

Complete genomic sequence analyses of Turnip mosaic virus basal-BR isolates from China

“…The presence of insertion in the plasmid was confirmed by PCR and restriction enzyme digestion before sequencing by the dideoxyl chain reaction termination method in Shanghai Invitrogen Biotechnology Co. Ltd. For each amplified fragment, two clones from independent PCR reactions were sequenced twice on both strands. If there was difference at any position of the sequences, at least four more clones were sequenced to obtain the consensus sequence [23]. At least six clones for the fragments obtained from 5 0 -RACE were sequenced.…”