Nucleotide sequence of cDNA clones encoding the complete precursor for subunit delta of thylakoid-located ATP synthase from spinach

Abstract

The nucleotide sequence of the entire nuclear-encoded precursor for subunit delta of the ATP synthase from spinach thylakoid membranes was determined by cDNA sequencing. Appropriate recombinant DNAs were selected from pBR322 and lambda gt11 libraries made from polyadenylated RNA of greening spinach seedlings. The mature protein consists of 187 amino acid residues corresponding to a molecular weight of 20468. The precursor protein (257 amino acid residues; M r=27676) is probably processed between a Met-Val bond. The predicted secondary structure of the transit sequence (70 residues; 7.2 kDa) resembles that of the Rieske Fe/S polypeptide, but shows little similarity with those of stromal or luminal proteins. The comparison of the chloroplast delta amino acid sequence with the published delta sequences from respiratory ATP synthases of bacterial and mitochondrial sources and from the thylakoid ATP synthase of the cyanobacterium Synechococcus suggests substantial divergence at the genic level although structural elements appear to be remarkably conserved.

Plant mitochondrial F1-ATPase. The presence of oligomycin-sensitivity-conferring protein (OSCP)

“…The sequence similarity is even greater when the sequence obtained is compared with the N-terminal sequence of sweet-potato 6-subunit (Kimura et al 1989), which also has an N-terminal extension of six residues when compared with the bovine OSCP. The similarity to the Escherichia coli (Walker et al, 1984) and spinach (Spinacia oleracea) chloroplast CF1 (Hermans et al, 1988) 6-subunits is less than that to bovine OSCP.…”

Isolation of CF0CF1 from Chlamydomonas reinhardtii cw15 and the N‐terminal amino acid sequences of the CF0CF1 subunits

“…Photosynthetic membranes contain four different F0 subunits I (b), II (b'), III (c~ 10), IV (a) and five different Ft subunits in a stoichiometry of ~Sf13~E are present in FoFi-ATPases [1]. in higher plants six CFoF ~ subunits namely 5, fl, e, I, III and IV are encoded in the plastid genome and three of the subunits, namely ~', ~ and II are nuclear encoded [2]. The same gene dibtribution is assumed for green algae.…”

Organization of plastid-encoded ATPase genes and flanking regions including homologues of infB and tsf in the thermophilic red alga Galdieria sulphuraria

“…This gene order is conserved in genomes of land plant chloroplasts [17]. However, genes for subunits b (atpG), 6 (atpD) and 7 (atpC) are nuclear-encoded in higher plants, probably due to gene transfer from the plastid genome to the nucleus [ 18,35,50]. AtpF shows an intron which is absent in other algae [4,22].…”