Purified pea (Pisum sativum) cotyledon Fl-ATPase contains six subunits rather than the five usually reported for F1-ATPases. The additional 26.5 kDa (a) subunit is shown by immunoblotting and N-terminal amino acid sequencing to be similar to bovine oligomycin-sensitivity-conferring protein (OSCP). It is concluded that the a subunit of plant mitochondrial Fl-ATPase is the plant OSCP. This OSCP subunit occurs in all mono-and di-cotyledonous species of plants tested (maize, oats, peas, potatoes, sweet potatoes and turnips).
Poly A+ RNA has been purified from phorbol myristate acetate stimulated mouse EL4 cells. Translation, by microinjection into frog oocytes, gave biologically active interleukin 2 (IL2) and colony stimulating factor for granulocytes and macrophages (GM-CSF). These two mRNAs were separated by centrifugation through linear sucrose gradients. The sedimentation coefficients for IL2 and GM-CSF mRNAs were found to be 11.5S and 8S respectively. The clonal and cellular morphologies induced by the oocyte material corresponded to low concentrations of authentic GM-CSF.
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