Nucleotide sequence and structural organization of Yersinia pestis insertion sequence IS100

Подладчикова, О. Н.

doi:10.1016/0378-1097(94)90302-6

Cited by 21 publications

(23 citation statements)

References 13 publications

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“…All Y. pseudotuberculosis strains which had hybridized with probe C were found to be Pst s , whereas no strain which failed to hybridize showed sensitivity to pesticin ( In this study, which included both serogroup I and serogroup III isolates, neither pesticin sensitivity nor IS100 carriage in Y. pseudotuberculosis was restricted to the serogroup I isolates, as has been observed by others (7,9,20,24). However, geographic heterogeneity has been reported for the distribution of the ypm gene within serotypes in Y. pseudotuberculosis isolates from Europe and the Far East (33), and it is possible that the probe-positive Y. pseudotuberculosis isolates that we have identified represent a geographic population of clonal variants.…”

supporting

confidence: 75%

Homology with a repeated Yersinia pestis DNA sequence IS100 correlates with pesticin sensitivity in Yersinia pseudotuberculosis

McDonough

Hare

1997

J Bacteriol

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We have identified IS100 sequences in a specific subset of Yersinia pseudotuberculosis isolates that were also sensitive to the Y. pestis-produced bacteriocin, pesticin. In contrast, Y. pseudotuberculosis strains which did not contain IS100 sequences were not sensitive to pesticin. We propose that IS100 serves as a molecular marker that identifies a subset of Y. pseudotuberculosis isolates that have a particularly close evolutionary and/or ecological relationship with Y. pestis.Yersinia pestis, the causative agent of bubonic plague (1, 21), and Y. pseudotuberculosis are closely related at the genetic level, having more than 90% DNA homology overall (3,14,18). Despite this genetic similarity, these two species differ greatly in their means of transmission and disease presentation, indicating that important genotypic differences between the agents must also exist. Unlike plague, Y. pseudotuberculosis infection is rarely fatal and cannot be transmitted by fleas (32). Understanding the factors that differentiate Y. pestis from Y. pseudotuberculosis at the molecular and genetic levels may facilitate plague control efforts and provide insight into the evolution of pathogenic microorganisms.In previous studies, we identified two contiguous DNA fragments (probe fragments B and C) from the 9.5-kbp "pesticin plasmid," pKYP1, that hybridized with all Y. pestis isolates and a subset of Y. pseudotuberculosis strains that we tested (16). We were intrigued by the possibility that these DNA probes may identify a subset of Y. pseudotuberculosis isolates that are more closely related to Y. pestis than others and undertook a study to characterize further the nature of these cross-hybridizing DNA sequences. We now report that DNA probe fragments B and C encode IS100 and that the presence of this repeated sequence correlates with pesticin sensitivity in a clonal subset of Y. pseudotuberculosis isolates. In addition, we suggest that the distinctive and variable restriction fragment length polymorphism (RFLP) patterns observed with Y. pestis and Y. pseudotuberculosis make IS100 useful as a molecular epidemiological tool in investigating the spread of these organisms in the environment.Characterization of DNA probe fragment C. Initial experiments to define the boundaries and specificity of the crosshybridizing DNA sequences within fragment C were performed by colony blot hybridization analysis as described previously (16). Except as noted, Y. pestis and Y. pseudotuberculosis were collected during plague surveillance operations and were generously provided by T. Quan, Division of Viral and Vector-Borne Disease, Centers for Disease Control and Prevention (CDC), Fort Collins, Colo. All Y. pseudotuberculosis isolates were phenotypically Lcr ϩ when tested on magnesium-oxalate agar for calcium dependence (12,17). Yersinia species were grown at 28ЊC in brain heart infusion broth or on solid agar (Difco Laboratories, Detroit, Mich.). All of 27 Y. pestis and 12 (36%) of 33 Y. pseudotuberculosis isolates hybridized with the DNA probe fragment C....

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supporting

confidence: 75%

Homology with a repeated Yersinia pestis DNA sequence IS100 correlates with pesticin sensitivity in Yersinia pseudotuberculosis

McDonough

Hare

1997

J Bacteriol

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“…2). The polypeptides predicted by these ORFs were found to be related to the two polypeptides encoded by the istA and istB genes of IS21 (34) and to the corresponding polypeptides in three further known relatives of IS21, namely, IS232 (23), IS5376 (51), and IS100 (27). On the basis of the similarity of the IS1326 ORFs to IstA and IstB from IS21, which have been shown to be required for transposition (33), the IS1326 genes are also designated istA and istB.…”

Section: Maps Of the Region Between The 3-cs And The Integron Boundarmentioning

confidence: 98%

The integrons In0, In2, and In5 are defective transposon derivatives

1996

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The class 1 integrons In0, In2, and In5, found in different locations in pVS1, Tn21, and pSCH884, have closely related structures. All three integrons contain an insertion sequence, IS1326, that is a new member of the IS21 family. IS1326 has caused deletions of adjacent 3-conserved segment and transposition module sequences, and all three integrons retain a complete copy of only one of four genes required for transposition of related transposons and are thus defective transposon derivatives. In2 contains an additional insertion sequence, IS1353, located within IS1326. IS1353 is a member of the IS3 family and appears to have been acquired after the integron was inserted into an ancestral mercury resistance transposon to create the ancestor of Tn21 and several other transposons that are close relatives of Tn21.

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“…Genotyping of Y. pestis was also accomplished by restriction fragment length polymorphism (RFLP) detected on Southern blots using probes originating from IS100, which is found at least singly on each of the three plasmids of the species and as multiple copies within the chromosome (24). This insertion sequence (IS) element was sequenced (11,23) and then used by Filippov et al (9) in conjunction with the novel IS285 to obtain fingerprint patterns establishing phylogenetic relationships. Further IS100-and IS285-based RFLP analysis became the method of choice used by many researchers for extensive analysis of Y. pestis collections (1,5,14,20).…”

mentioning

confidence: 99%

Genetic Variability of Yersinia pestis Isolates as Predicted by PCR-Based IS 100 Genotyping and Analysis of Structural Genes Encoding Glycerol-3-Phosphate Dehydrogenase ( glpD )

et al. 2002

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A PCR-based genotyping system that detects divergence of IS100 locations within the Yersinia pestis genome was used to characterize a large collection of isolates of different biovars and geographical origins. Using sequences derived from the glycerol-negative biovar orientalis strain CO92, a set of 27 locus-specific primers was designed to amplify fragments between the end of IS100 and its neighboring gene. Geographically diverse members of the orientalis biovar formed a homogeneous group with identical genotype with the exception of strains isolated in Indochina. Yersinia pestis, the causative agent of bubonic plague, is a recently evolved clone of Yersinia pseudotuberculosis serotype O:1b (1, 29). Strains of Y. pestis are divided into three biovars on the basis of their abilities to ferment glycerol and to reduce nitrate. These phenotypic differences have proven useful in distinguishing strains thought to be responsible for the three plague pandemics (7). Isolates of the biovar antiqua (able to ferment glycerol and reduce nitrate) are believed to remain as holdovers from the first pandemic that started with the Justinian plague of the 6th century. Strains of the biovar medievalis (glycerol positive and nitrate negative) evidently caused the second pandemic of Europe (Black Death), which was initiated during the 14th century, and strains of the biovar orientalis (glycerol negative and nitrate positive) are responsible for the third plague pandemic of modern times (15,22).Several molecular methods that generate fingerprinting patterns of Y. pestis DNA have been successfully used for genotyping strains of this microorganism, such as pulse-field gel electrophoresis and ribotyping (15,16,18,25) as well as newly described variable-number tandem repeat analysis (2). Genotyping of Y. pestis was also accomplished by restriction fragment length polymorphism (RFLP) detected on Southern blots using probes originating from IS100, which is found at least singly on each of the three plasmids of the species and as multiple copies within the chromosome (24). This insertion sequence (IS) element was sequenced (11, 23) and then used by Filippov et al. (9) in conjunction with the novel IS285 to obtain fingerprint patterns establishing phylogenetic relationships. Further IS100-and IS285-based RFLP analysis became the method of choice used by many researchers for extensive analysis of Y. pestis collections (1,5,14,20). Finally, a third insertion sequence (IS1541) was discovered that disrupts inv, which encodes invasin (28). This element is now known to be present in many copies in the Y. pestis genome and has also been used to obtain RFLP fingerprinting profiles (21, 28).Many insertions of IS1541 in the genome of Y. pestis strain 6/69 M biovar orientalis were characterized and then tested to see if the insert flanked the same genes in other strains of Y. pestis (21). The authors employed five unrelated strains (four of biovar orientalis and one of medievalis) of different ribotypes and IS1541 hybridization patterns that were isol...

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Nucleotide sequence and structural organization of Yersinia pestis insertion sequence IS100

Cited by 21 publications

References 13 publications

Homology with a repeated Yersinia pestis DNA sequence IS100 correlates with pesticin sensitivity in Yersinia pseudotuberculosis

Homology with a repeated Yersinia pestis DNA sequence IS100 correlates with pesticin sensitivity in Yersinia pseudotuberculosis

The integrons In0, In2, and In5 are defective transposon derivatives

Genetic Variability of Yersinia pestis Isolates as Predicted by PCR-Based IS 100 Genotyping and Analysis of Structural Genes Encoding Glycerol-3-Phosphate Dehydrogenase ( glpD )

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