Nucleoside-Based Ultrasensitive Fluorescent Probe for the Dual-Mode Imaging of Microviscosity in Living Cells

Abstract

Microviscosity changes of living cells have a far-reaching influence on diffusion and movement capacity of RNA and, more seriously, could modify RNA functions in living cells. Fluorescent rotor, whose fluorescence responds to different environmental viscosities, holds great potential for the imaging of viscosity in biosystem. Although many fluorescent rotors have been reported for viscosity, the fluorogenic rotor with ultrasensitivity for the determination of microviscosity (<10 cP) was rarely reported. Herein, we report a nucleoside-based two-photon fluorescent rotor (dABp-3) that can selectively and ultrasensitively image microviscosity in RNA region of living cells for the first time. 2'-Deoxyadenosine is selected as an electron donor to permit energy transfer via the acetylenic bond to acceptor, a typical boron dipyrromethene moiety. Another highlight, dABp-3 is based on 2'-deoxyadenosine, which result in its recognition capacity for RNA. dABp-3 with ultrasensitivity provides a varied linear response to the microrange viscosity (1.8-6.0 cP) in RNA region of living cells on dual-mode-two-photon ratio mode and fluorescence lifetime mode. After screening and optimization, advantageously, dABp-3 can be used to screen reticulocytes from mature blood cells of thrombosis models in vitro and in vivo because of targeting RNA, while simultaneously image microviscosity changes in these cells. So, dABp-3 as an analytical tool holds considerable promise for bioimaging and monitoring of microviscosity changes in complex biological systems.

Luminescent metal salen complex as intracellular microviscosity fluorescent sensor

“…Salen] + Cl − 在细胞内呈多亚细胞器分布(图7). 细胞内的微黏度会随着所处环境温度的降低而 升高 [19,22,37] . 使用自噬诱导剂雷帕霉素 [38] 或者血清饥饿 [29] 参考文献…”

Functionalized BODIPYs as Fluorescent Molecular Rotors for Viscosity Detection

“…(D) The fluorescence lifetime measurements of BODIPY 14 (3.0 μM) with different viscosities. Reproduced with permission from Li et al (2016), Copyright 2016 American Chemical Society. (E) 2,6-Functionalized BODIPY 15 as a FMR.…”

“…Li et al reported a RNA-targeted BODIPY 14 as a new FMR to detect intracellular viscosity (Figure 2C; Li et al, 2016). FMR 14 showed two different maximum wavelengths at 496 and 565 nm, respectively.…”

Proof of principle of a purine D–A–D′ ligand based ratiometric chemical sensor harnessing complexation induced intermolecular PET

“…The purine derivatives have already been demonstrated as emitters in OLEDs, [18][19][20] as pH 21 sensors or even as imaging tools for cell compartments 22 and their environment. 23 Additionally, the purine derivatives are naturallike molecules that can be more easily recognized by cells. 14 During the last decade a few examples of purines bearing traditional complexing structures like crown ethers, 24 pincer forming substituents 25 and salicyl-imine side chains 22 have been reported and applied as metal ion sensors.…”