Nucleolar organizer regions (NORs) and myoepitheliomas: a comparison with DNA content and clinical course

Carrillo, Rosario; El‐Naggar, Adel K.; Luna, Mario A.; Rodríguez‐Peralto, José Luis; Batsakis, John G.

doi:10.1017/s0022215100120341

Cited by 10 publications

(4 citation statements)

References 33 publications

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“…Although our patient population is small, our findings show that the number of AgNORs is not a useful prognostic indicator in CMM. Similar lack of correlation between AgNOR values and clinical outcome has been shown recently in other nonmelanocytic tumors (28)(29)(30)(31)(32). Based on our observations ancl those of others, we suggest that Ag-NOR count has little or no significance as a prognostic marker in thick melanomas.…”

Section: Discussionsupporting

confidence: 85%

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Prognostic significance of nucleolar organizer regions (NORS) in malignant melanoma

McDonald

et al. 1994

J Cutan Pathol

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Nucleolar organizer regions (NORs) are loops of ribosomal DNA (rDNA) in the nucleolus and are associated with acidic proteins. They are seen in routinely processed paraffin sections by using a one‐step colloidal silver (Ag) staining method; they appear as black dots termed “AgNORs”. The quantitative assay of AgNORs has been used to differentiate benign from malignant neoplasms. Melanocytic lesions differ significantly in AgNOR counts between malignant melanoma and nevi. However, conflicting results have been reported as to AgNORs' prognostic value in melanoma. A recent study showed AgNOR counts to be a more accurate prognostic indicator than Breslow's thickness. In this study, we counted the AgNORs in 26 patients with primary cutaneous melanomas (CMM) between 2.0 mm and 2.5 mm thick. Of these, 14 are alive without disease (AN) at 5 years after diagnosis (group 1), and 12 are dead of disease (DD) in less than 5 years (group 2). The AgNORs were scored in 30 nuclei per tumor, and the means were calculated. For group 1, the mean number of AgNORs per nucleus was 6.88, ranging from 3.73 to 12.70. For group 2, the mean number was 6.97, ranging from 3.63 to 11.67. Statistical analysis using analysis of variance (ANOVA) showed no significant difference between the groups (p=0.33). In our study, AgNOR counts did not prove to be of prognostic value in malignant melanoma. Ronan SG, Farolan MJ, McDonald A, Manaligod JR, Das Gupta TK. Prognostic significance of nucleolar organizer regions (NORs) in malignant melanoma.

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Section: Discussionsupporting

confidence: 85%

“…Previous sttidies have shown strong coi relalion between AgNOR scores and proliferation indices (17)(18)(19)(20), mitotic ecmnts (21), Itimor grade (22,23), fiow cytometric DNA content (17,20,24,25), and aggie.s.sive biologic behavior (26,27). Other studies have shown the ccjntraiy (28)(29)(30)(31)(32).…”

Section: Discussionmentioning

confidence: 91%

Prognostic significance of nucleolar organizer regions (NORS) in malignant melanoma

McDonald

et al. 1994

J Cutan Pathol

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“…13 However, Carrillo et al found no correlation between nucleolar organizer regions (an indicator of cell proliferative activity) and clinical outcome in benign and malignant myoepithelioma. 27 According to our results, MMEs that showed high proliferative activity, extensive invasion into the surrounding tissue, perineural permeation, and marked cellular pleomorphism had a poor prognosis. In salivary gland tumors, it has been reported that p53 expression is related to histologic type and prognosis.…”

Section: Discussionmentioning

confidence: 53%

Salivary gland malignant myoepithelioma

Nagao

Sugano

Ishida

et al. 1998

Cancer

260

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“…Data characterizing cellular DNA content of myoepithelioma have been scarce in the literature. In a study of Carrillo et al, 5 all the grade I myoepitheliomas (encapsulated tumors without cellular atypia) were diploid, with low proliferative activity (less than 4% of cells in S-phase). None of these tumors recurred.…”

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confidence: 95%

Myoepithelioma of the Submandibular Gland Occurring after Cardiac Transplantation

1997

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Myoepithelioma is a tumor composed entirely or almost exclusively of myoepithelial cells. It is an uncommon neoplasm, accounting for less than 1% of all salivary gland tumors. Immunosuppressed recipients of human donor organ grafts have an increased incidence of neoplasia.1 This represents an effect of long-term immunosuppressive therapy. In the present report we present a case of myoepithelioma of the submandibular gland occurring in a cardiac-transplanted man. To the best of our knowledge no case of such an association has been reported.A 61-year-old man underwent a heart transplantation in March 1992. He required it for the treatment of severe left ventricular failure after cardiac valve surgery. The patient has been treated since 1992 with an immunosuppressive regimen consisting of a combination of CSA, prednisone and aziathoprine. On June 1995, he was admitted to the hospital for excision of an asymptomatic mass in the right submandibular gland. The mass had been present for 14 months after heart transplantation. Physical examination revealed a firm, non-tender, nonfluctuant and freely movable mass. Excision of the gland was performed.Gross examination of the submandibular gland showed at the transection a well-circumscribed nodule, 13 cm in diameter, embedded in the gland. The tumor was whitish, firm and it showed a paracentral cyst filled with mucoid fluid. The remains of the gland were normal. Microscopically, a well-delineated tumor mass was separated from the surrounding salival gland by a thick hyalinized connective tissue capsule. The neoplasm was solidly packed with uniform epithelial cells and a scant, hyalinized stroma ( Figure 1). The tumor cells were spindle-shaped, with centrally placed nuclei containing delicately dispersed chromatin and a thin nuclear envelope. The nuclei occupied most of the cell bodies. The cells were bipolar, with slightly eosinophilic cytoplasm which varied from granular to fibrillar. The stroma was characterized by the presence of an amount of amorphous and acellular material in which a few fibroblasts and small collagen fibers were scattered. The tumor contained a few small isolated intercalated ducts; they were lined with a single layer of small cells with round and dense nuclei. The number of ductal structures was 0.4 in every 220x magnification field. The central cyst was lined by a single layer of flattened ductal epithelium. The neoplastic cells were diffusely positive for anti-human alpha-smooth muscle actin, anti-vitamin, and anti-S-100 protein, and were negative for anti-keratin cocktail CK22. The DNA histogram showed a diploid pattern, GO-GI, S and G2+M phase fractions were 94.5%, 3.4% and 2.1% respectively. Coefficient of variation was 8.9%. With all these findings, the pathological diagnosis was spindle-cell myoepithelioma.The incidence of the novo neoplasms in patients who have received solid organ transplants is of 3% to 11% within the first year and up to 21% after five years.2 Lanza et al. 3 reported a 10% incidence of malignant neoplasms in 53 patients w...

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Nucleolar organizer regions (NORs) and myoepitheliomas: a comparison with DNA content and clinical course

Cited by 10 publications

References 33 publications

Prognostic significance of nucleolar organizer regions (NORS) in malignant melanoma

Prognostic significance of nucleolar organizer regions (NORS) in malignant melanoma

Salivary gland malignant myoepithelioma

Myoepithelioma of the Submandibular Gland Occurring after Cardiac Transplantation

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