Nucleocytoplasmic Trafficking of the Arabidopsis WD40 Repeat Protein XIW1 Regulates ABI5 Stability and Abscisic Acid Responses

Xu, Xuezhong; Wan, Wang; Jiang, Guobin; Xi, Yue; Huang, Hai‐Jian; Cai, Jiajia; Chang, Yanan; Duan, Cheng‐Guo; Mangrauthia, Satendra K.; Peng, Xinxiang; Zhu, Jian‐Kang; Zhu, Guiyu

doi:10.1016/j.molp.2019.07.001

Cited by 60 publications

(40 citation statements)

References 51 publications

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“…Abscisic acid (ABA) regulates diverse cellular and molecular processes during plant development and in response to abiotic stresses (Xu et al, 2019; Chen et al, 2020; Qi et al, 2020). Abscisic acid is recognized by receptors including PYRABACTIN RESISTANCE/PYRABACTIN RESISTANCE‐LIKE/REGULATORY COMPONENT OF ABSCISIC ACID RECEPTOR (PYR/PYL/RCAR) family proteins, which combine with clade A PROTEIN PHOSPHATASES OF TYPE 2C (PP2C) to function as coreceptors for ABA (Fujita et al, 2009; Umezawa et al, 2009; Gonzalez‐Guzman et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Abscisic acid signaling negatively regulates nitrate uptake via phosphorylation of NRT1.1 by SnRK2s in Arabidopsis

Deng

et al. 2021

JIPB

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Nitrogen (N) is a limiting nutrient for plant growth and productivity. The phytohormone abscisic acid (ABA) has been suggested to play a vital role in nitrate uptake in fluctuating N environments. However, the molecular mechanisms underlying the involvement of ABA in N deficiency responses are largely unknown. In this study, we demonstrated that ABA signaling components, particularly the three subclass III SUCROSE NON‐FERMENTING1 (SNF1)‐RELATED PROTEIN KINASE 2S (SnRK2) proteins, function in root foraging and uptake of nitrate under N deficiency in Arabidopsis thaliana. The snrk2.2snrk2.3snrk2.6 triple mutant grew a longer primary root and had a higher rate of nitrate influx and accumulation compared with wild‐type plants under nitrate deficiency. Strikingly, SnRK2.2/2.3/2.6 proteins interacted with and phosphorylated the nitrate transceptor NITRATE TRANSPORTER1.1 (NRT1.1) in vitro and in vivo. The phosphorylation of NRT1.1 by SnRK2s resulted in a significant decrease of nitrate uptake and impairment of root growth. Moreover, we identified NRT1.1Ser585 as a previously unknown functional site: the phosphomimetic NRT1.1S585D was impaired in both low‐ and high‐affinity transport activities. Taken together, our findings provide new insight into how plants fine‐tune growth via ABA signaling under N deficiency.

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Section: Introductionmentioning

confidence: 99%

Abscisic acid signaling negatively regulates nitrate uptake via phosphorylation of NRT1.1 by SnRK2s in Arabidopsis

Deng

et al. 2021

JIPB

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“…Ilmi), 20 seedlings planted in 10 different plates, were grown in the dark for 10 days and transferred to light conditions for 10 h. Rice protoplast preparation and transient gene expression were performed as described previously [ 51 ]. For transient expression of OsC3H10-GFP in rice protoplasts, the OsC3H10 coding sequence translationally fused with GFP (OsC3H10-GFP) was inserted into the pHBT vector carrying the 35S promoter [ 51 , 52 , 53 , 54 ]. Similarly, coding sequences of OsDCP1-2 and OsPABP8 were translationally fused with red fluorescent protein (RFP) and inserted into the pHBT vector.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of OsC3H10, a CCCH-Zinc Finger, Improves Drought Tolerance in Rice by Regulating Stress-Related Genes

Seong

Shim

Bang

et al. 2020

Plants

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CCCH zinc finger proteins are members of the zinc finger protein family, and are known to participate in the regulation of development and stress responses via the posttranscriptional regulation of messenger RNA in animals and yeast. However, the molecular mechanism of CCCHZF-mediated drought tolerance is not well understood. We analyzed the functions of OsC3H10, a member of the rice CCCHZF family. OsC3H10 is predominantly expressed in seeds, and its expression levels rapidly declined during seed imbibition. The expression of OsC3H10 was induced by drought, high salinity and abscisic acid (ABA). Subcellular localization analysis revealed that OsC3H10 localized not only in the nucleus but also to the processing bodies and stress granules upon stress treatment. Root-specific overexpression of OsC3H10 was insufficient to induce drought tolerance, while the overexpression of OsC3H10 throughout the entire plant enhanced the drought tolerance of rice plants. Transcriptome analysis revealed that OsC3H10 overexpression elevated the expression levels of genes involved in stress responses, including LATE EMBRYOGENESIS ABUNDANT PROTEINs (LEAs), PATHOGENESIS RELATED GENEs (PRs) and GERMIN-LIKE PROTEINs (GLPs). Our results demonstrated that OsC3H10 is involved in the regulation of the drought tolerance pathway by modulating the expression of stress-related genes.

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“…ABI5 S-nitrosylation at Cys153 residue promotes its interaction with CULLIN4based and KEEP ON GOING E3 ligases, leading to its degradation (Albertos et al, 2015). Xu et al (2019) also described the XIW1 nuclear import under oxidative treatments by using hydrogen peroxide, highlighting the relevance of redox conditions. In addition, the replacement of Cys260 by an alanine results in constitutive nuclear localization, establishing a clear role during nucleocytoplasmic protein shuttle.…”

Section: Cysteine Redox Sensors During Development and Stress Trade-offmentioning

confidence: 97%

“…In a recent study, Xu et al (2019) characterized a WD40containing protein, named XPO1-Interacting WD40 protein 1 (XIW1). XIW1 was isolated as a scaffold protein interacting with Exportin 1 (XPO1), a receptor mediating protein nuclear export.…”

Section: Wd40 Proteins Fine-tune Mobile Players During Aba Signalingmentioning

confidence: 99%

Protein Shuttle between Nucleus and Cytoplasm: New Paradigms in the ABI5-dependent ABA Responses

2019

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Plant survival depends on seed germination, progression through post-germinative developmental checkpoints, and adaptation to abiotic stresses. All these processes underlie a tightly controlled regulation by the stress hormone abscisic acid (ABA) at different levels, even though the main research focuses on changes in transcript and protein abundance. Nevertheless, protein fate undergoes complex readjustments that allow signal transduction to be integrated into physiological responses. Among them, protein redox status, stability, capacity of oligomerization, formation of complexes, and subcellular compartmentalization become key points for plant reprogramming against stresses to acquire an optimal development. In this Spotlight, we highlight and discuss the latest advances in dissecting the mechanisms driving protein dynamics of nucleocytoplasmic trafficking in the cell and its relevance for regulating the stability of ABI5 and the ABI5-dependent ABA signaling pathway (Figure 1).

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Nucleocytoplasmic Trafficking of the Arabidopsis WD40 Repeat Protein XIW1 Regulates ABI5 Stability and Abscisic Acid Responses

Cited by 60 publications

References 51 publications

Abscisic acid signaling negatively regulates nitrate uptake via phosphorylation of NRT1.1 by SnRK2s in Arabidopsis

Abscisic acid signaling negatively regulates nitrate uptake via phosphorylation of NRT1.1 by SnRK2s in Arabidopsis

Overexpression of OsC3H10, a CCCH-Zinc Finger, Improves Drought Tolerance in Rice by Regulating Stress-Related Genes

Protein Shuttle between Nucleus and Cytoplasm: New Paradigms in the ABI5-dependent ABA Responses

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