Protein Shuttle between Nucleus and Cytoplasm: New Paradigms in the ABI5-dependent ABA Responses

Sánchez-Vicente, Inmaculada; Albertos, Pablo; Lorenzo, Óscar

doi:10.1016/j.molp.2019.09.005

Cited by 4 publications

(2 citation statements)

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“…To assess seed stress level, responses of malondialdehyde (MDA), a marker of oxidative stress ( Kranner et al., 2010 ), and two transcription factors (TFs), ABI5 and ANAC089, with prominent roles in the regulation of seed germination were studied. The TF ABI5, a member of the basic leucine zipper (bZIP) family, represents a hub in the abscisic acid (ABA) signalling network ( Sanchez-Vicente et al., 2019 ) during seed germination and post-germinative developmental checkpoints ( Finkelstein and Lynch, 2000 ; Lopez-Molina et al., 2001 ). In barley seeds, the ABI5 protein is expressed in the aleurone cells ( Casaretto and Ho, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

Impacts of drought and elevated temperature on the seeds of malting barley

et al. 2022

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High seed quality is key to agricultural production, which is increasingly affected by climate change. We studied the effects of drought and elevated temperature during seed production on key seed quality traits of two genotypes of malting barley (Hordeum sativum L.). Plants of a “Hana-type” landrace (B1) were taller, flowered earlier and produced heavier, larger and more vigorous seeds that resisted ageing longer compared to a semi-dwarf breeding line (B2). Accordingly, a NAC domain-containing transcription factor (TF) associated with rapid response to environmental stimuli, and the TF ABI5, a key regulator of seed dormancy and vigour, were more abundant in B1 seeds. Drought significantly reduced seed yield in both genotypes, and elevated temperature reduced seed size. Genotype B2 showed partial thermodormancy that was alleviated by drought and elevated temperature. Metabolite profiling revealed clear differences between the embryos of B1 and B2. Drought, but not elevated temperature, affected the metabolism of amino acids, organic acids, osmolytes and nitrogen assimilation, in the seeds of both genotypes. Our study may support future breeding efforts to produce new lodging and drought resistant malting barleys without trade-offs that can occur in semi-dwarf varieties such as lower stress resistance and higher dormancy.

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Section: Introductionmentioning

confidence: 99%

Impacts of drought and elevated temperature on the seeds of malting barley

et al. 2022

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“…The PP2C Arabidopsis cluster includes nine members (i.e., ABI1, ABI2, HAB1, HAB2, AHG1, AHG3/AtPP2CA, HAI1, HAI2 and HAI3) which are negative regulators of early ABA signaling [ 17 , 31 , 32 , 33 ]. Removal of PP2C repression allows downstream signaling via OST1/SnRK2.6/SnRK2E SNF1-related protein kinases 2 (SnRK2) [ 32 , 33 , 34 , 35 ] ( Figure 1 ). The Arabidopsis genome contains 10 members of SnRK2 ; among them, SRK2D / SnRK2.2, SRK2E / OST1 / SnRK2.6 and SRK2I / SnRK2.3 are essential for ABA responses [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Auxin: Hormonal Signal Required for Seed Development and Dormancy

Matilla

2020

Plants

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The production of viable seeds is a key event in the life cycle of higher plants. Historically, abscisic acid (ABA) and gibberellin (GAs) were considered the main hormones that regulate seed formation. However, auxin has recently emerged as an essential player that modulates, in conjunction with ABA, different cellular processes involved in seed development as well as the induction, regulation and maintenance of primary dormancy (PD). This review examines and discusses the key role of auxin as a signaling molecule that coordinates seed life. The cellular machinery involved in the synthesis and transport of auxin, as well as their cellular and tissue compartmentalization, is crucial for the development of the endosperm and seed-coat. Thus, auxin is an essential compound involved in integuments development, and its transport from endosperm is regulated by AGAMOUS-LIKE62 (AGL62) whose transcript is specifically expressed in the endosperm. In addition, recent biochemical and genetic evidence supports the involvement of auxins in PD. In this process, the participation of the transcriptional regulator ABA INSENSITIVE3 (ABI3) is critical, revealing a cross-talk between auxin and ABA signaling. Future experimental aimed at advancing knowledge of the role of auxins in seed development and PD are also discussed.

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Deciphering the role of rapamycin in modulating decidual senescence: implications for decidual remodeling and implantation failure

Kendirci-Katirci,

Sati,

Celik-Ozenci

2024

J Assist Reprod Genet

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Purpose Physiological decidual senescence promotes embryo implantation, whereas pathological decidual senescence causes many pregnancy pathologies. The aim of this study was to evaluate the effect of rapamycin on decidual cell subpopulations and endometrial function in physiological and induced senescence and to investigate the decidual cell subpopulations present in physiological conditions during early pregnancy and implantation in mice. Methods Control, physiological decidualization (0.5 mM cAMP and 1 μM MPA added), and induced senescence (0.1 mM HU added) models with and without 200 nM rapamycin treatment were established using a human endometrial stromal cell line, and decidual cell subpopulations were analyzed by immunofluorescence and flow cytometry. The human extravillous trophoblast cell line AC-1M88 was also cultured in decidualization models, and spheroid expansion analysis was performed. In in vivo studies, decidual cell subpopulations were analyzed by immunofluorescence during early mouse pregnancy. Results The results revealed that rapamycin decreased DIO2 and β-GAL expressions in physiological and induced senescence without FOXO1. Notably, in induced senescence, increased fragmentation was observed in AC-1M88 cells, and rapamycin treatment successfully attenuated the fragmentation of spheroids. We showed that the FOXO1-DIO2 signaling axis can trigger decidual senescence during early gestation and days of implantation in mice. Conclusions Our study underlines the importance of rapamycin in modulating decidual cell subpopulations and endometrial tissue function during decidual senescence. The information obtained may provide insight into the pathologies of pregnancy seen due to decidual senescence and guide better treatment strategies for reproductive problems.

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Protein Shuttle between Nucleus and Cytoplasm: New Paradigms in the ABI5-dependent ABA Responses

Cited by 4 publications

References 12 publications

Impacts of drought and elevated temperature on the seeds of malting barley

Impacts of drought and elevated temperature on the seeds of malting barley

Auxin: Hormonal Signal Required for Seed Development and Dormancy

Deciphering the role of rapamycin in modulating decidual senescence: implications for decidual remodeling and implantation failure

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