Overexpression of OsC3H10, a CCCH-Zinc Finger, Improves Drought Tolerance in Rice by Regulating Stress-Related Genes

Seong, So Yoon; Shim, Jae Sung; Bang, Seung Woon; Kim, Ju-Kon

doi:10.3390/plants9101298

Cited by 28 publications

(37 citation statements)

References 57 publications

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“…To function, proteins must be localized to the appropriate part of the cell; in turn, a protein’s location in a cell can offer clues about its function [ 99 , 100 ]. So far, CCCH zinc-finger proteins show different patterns of localization: some are located in the nucleus, such as GhZFP1 [ 72 ], AtTZF11 [ 101 ], OsDOS [ 84 ], AtZFP1 [ 21 ], KHZ1 and KHZ2 [ 102 ], SAW1 [ 103 ] and OsC3H10 [ 104 ]. Some are located in the cytoplasm plasma membrane, such as Oxidation-related Zinc Finger 1 (AtOZF1) [ 105 ] and AtOZF2 [ 106 ].…”

Section: Subcellular Localization Of Ccch Zinc-finger Proteinsmentioning

confidence: 99%

“…The widespread presence of shuttle signals in plant CCCH proteins suggests that they may be intra- and extranuclear shuttle proteins with important roles in signal transduction and stress responses [ 65 ]. In addition, AtTZF1, AtTZF4, AtTZF5, AtTZF6 and OsC3H10 co-localize with processing bodies (PBs) and stress granules (SGs) in the cytoplasm [ 104 ]. As aggregates of cytoplasmic messenger ribonucleoprotein complexes, PBs and SGs play important roles in the post-transcriptional regulation of genes and have been highly conserved during evolution.…”

Section: Subcellular Localization Of Ccch Zinc-finger Proteinsmentioning

confidence: 99%

“…PBs and SGs play an important role in plant tolerance to abiotic stress [ 97 ]. AtTZF1, AtTZF4, AtTZF5, and AtTZF6 may function through a mechanism associated with PBs and SGs, and this function may be related to the cytoplasmic shuttle characteristics of these CCCH proteins [ 104 , 114 ].…”

Section: Subcellular Localization Of Ccch Zinc-finger Proteinsmentioning

confidence: 99%

“…In Aegilops tauschii Coss., 36 CCCH zinc-finger family genes have been identified by comprehensive computational analysis, among which AetTZF1 shows the strongest expression under drought stress [ 67 ]. Drought and ABA could significantly induce the expression of OsC3H10 in rice [ 104 ].…”

Section: The Roles Of Ccch Zinc-finger Proteins In Abiotic Stressmentioning

confidence: 99%

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The Roles of CCCH Zinc-Finger Proteins in Plant Abiotic Stress Tolerance

Han

Qiao

et al. 2021

IJMS

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Zinc-finger proteins, a superfamily of proteins with a typical structural domain that coordinates a zinc ion and binds nucleic acids, participate in the regulation of growth, development, and stress adaptation in plants. Most zinc fingers are C2H2-type or CCCC-type, named after the configuration of cysteine (C) and histidine (H); the less-common CCCH zinc-finger proteins are important in the regulation of plant stress responses. In this review, we introduce the domain structures, classification, and subcellular localization of CCCH zinc-finger proteins in plants and discuss their functions in transcriptional and post-transcriptional regulation via interactions with DNA, RNA, and other proteins. We describe the functions of CCCH zinc-finger proteins in plant development and tolerance to abiotic stresses such as salt, drought, flooding, cold temperatures and oxidative stress. Finally, we summarize the signal transduction pathways and regulatory networks of CCCH zinc-finger proteins in their responses to abiotic stress. CCCH zinc-finger proteins regulate the adaptation of plants to abiotic stress in various ways, but the specific molecular mechanisms need to be further explored, along with other mechanisms such as cytoplasm-to-nucleus shuttling and post-transcriptional regulation. Unraveling the molecular mechanisms by which CCCH zinc-finger proteins improve stress tolerance will facilitate the breeding and genetic engineering of crops with improved traits.

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Section: Subcellular Localization Of Ccch Zinc-finger Proteinsmentioning

confidence: 99%

Section: Subcellular Localization Of Ccch Zinc-finger Proteinsmentioning

confidence: 99%

Section: Subcellular Localization Of Ccch Zinc-finger Proteinsmentioning

confidence: 99%

Section: The Roles Of Ccch Zinc-finger Proteins In Abiotic Stressmentioning

confidence: 99%

See 2 more Smart Citations

The Roles of CCCH Zinc-Finger Proteins in Plant Abiotic Stress Tolerance

Han

Qiao

et al. 2021

IJMS

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“…AtTZF14 and AtTZF15 / AtCDM1 are involved in secondary wall thickening and anther development [ 8 ]. In rice ( Oryza sativa ), expression of OsTZF1 is upregulated by drought, salt stress, and hydrogen peroxide [ 9 ]; OsC3H10 is involved in drought tolerance by modulating the expression of stress-related genes [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Non-TZF Protein AtC3H59/ZFWD3 Is Involved in Seed Germination, Seedling Development, and Seed Development, Interacting with PPPDE Family Protein Desi1 in Arabidopsis

Seok

Bae

Kim

et al. 2021

IJMS

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Despite increasing reports on the function of CCCH zinc finger proteins in plant development and stress response, the functions and molecular aspects of many non-tandem CCCH zinc finger (non-TZF) proteins remain uncharacterized. AtC3H59/ZFWD3 is an Arabidopsis non-TZF protein and belongs to the ZFWD subfamily harboring a CCCH zinc finger motif and a WD40 domain. In this study, we characterized the biological and molecular functions of AtC3H59, which is subcellularly localized in the nucleus. The seeds of AtC3H59-overexpressing transgenic plants (OXs) germinated faster than those of wild type (WT), whereas atc3h59 mutant seeds germinated slower than WT seeds. AtC3H59 OX seedlings were larger and heavier than WT seedlings, whereas atc3h59 mutant seedlings were smaller and lighter than WT seedlings. Moreover, AtC3H59 OX seedlings had longer primary root length than WT seedlings, whereas atc3h59 mutant seedlings had shorter primary root length than WT seedlings, owing to altered cell division activity in the root meristem. During seed development, AtC3H59 OXs formed larger and heavier seeds than WT. Using yeast two-hybrid screening, we isolated Desi1, a PPPDE family protein, as an interacting partner of AtC3H59. AtC3H59 and Desi1 interacted via their WD40 domain and C-terminal region, respectively, in the nucleus. Taken together, our results indicate that AtC3H59 has pleiotropic effects on seed germination, seedling development, and seed development, and interacts with Desi1 in the nucleus via its entire WD40 domain. To our knowledge, this is the first report to describe the biological functions of the ZFWD protein and Desi1 in Arabidopsis.

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