The Roles of CCCH Zinc-Finger Proteins in Plant Abiotic Stress Tolerance

Han, Guoliang; Qiao, Ziqi; Li, Yuxia; Wang, Chengfeng; Wang, Baoshan

doi:10.3390/ijms22158327

Cited by 73 publications

(71 citation statements)

References 198 publications

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“…Several studies have shown that CCCH proteins are involved in stress tolerance in plants [ 57 ]. For example, Arabidopsis AtSZF1 and AtSZF2 , two closely related CCCH zinc finger genes, negatively regulate the expression of salt-responsive genes and modulate the tolerance to salt stress [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

CCCH Zinc finger genes in Barley: genome-wide identification, evolution, expression and haplotype analysis

Pan

Zeng

et al. 2022

BMC Plant Biol

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Background CCCH transcription factors are important zinc finger transcription factors involved in the response to biotic and abiotic stress and physiological and developmental processes. Barley (Hordeum vulgare) is an agriculturally important cereal crop with multiple uses, such as brewing production, animal feed, and human food. The identification and assessment of new functional genes are important for the molecular breeding of barley. Results In this study, a total of 53 protein-encoding CCCH genes unevenly dispersed on seven different chromosomes were identified in barley. Phylogenetic analysis categorized the barley CCCH genes (HvC3Hs) into eleven subfamilies according to their distinct features, and this classification was supported by intron–exon structure and conserved motif analysis. Both segmental and tandem duplication contributed to the expansion of CCCH gene family in barley. Genetic variation of HvC3Hs was characterized using publicly available exome-capture sequencing datasets. Clear genetic divergence was observed between wild and landrace barley populations in HvC3H genes. For most HvC3Hs, nucleotide diversity and the number of haplotype polymorphisms decreased during barley domestication. Furthermore, the HvC3H genes displayed distinct expression profiles for different developmental processes and in response to various types of stresses. The HvC3H1, HvC3H2 and HvC3H13 of arginine-rich tandem CCCH zinc finger (RR-TZF) genes were significantly induced by multiple types of abiotic stress and/or phytohormone treatment, which might make them as excellent targets for the molecular breeding of barley. Conclusions Overall, our study provides a comprehensive characterization of barley CCCH transcription factors, their diversity, and their biological functions.

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Section: Discussionmentioning

confidence: 99%

CCCH Zinc finger genes in Barley: genome-wide identification, evolution, expression and haplotype analysis

Pan

Zeng

et al. 2022

BMC Plant Biol

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“…On the basis of the number and order of the Cys and His residues that bind the Zinc ion in the secondary structure of the finger, they were divided into several different types, including C2H2, C2C2, C2HC, C2C2C2C2, and C2HCC2C2 [ 7 , 8 , 9 ]. Among the different zinc-finger proteins, Cys2/His2 (C2H2)-type (TFIII-types) was a characteristic representative of eukaryotic transcription factors to abiotic stress [ 10 , 11 , 12 , 13 , 14 , 15 ]. Di19 (drought-induced 19) protein containing two typical Cys2/His2 zinc finger domains is one of the Cys2/His2-type TFs.…”

Section: Introductionmentioning

confidence: 99%

Homologous Drought-Induced 19 Proteins, PtDi19-2 and PtDi19-7, Enhance Drought Tolerance in Transgenic Plants

Lin

Chen

et al. 2022

IJMS

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Drought-induced 19 (Di19) proteins play important roles in abiotic stress responses. Thus far, there are no reports about Di19 family in woody plants. Here, eight Di19 genes were identified in poplar. We analyzed phylogenetic tree, conserved protein domain, and gene structure of Di19 gene members in seven species. The results showed the Di19 gene family was very conservative in both dicotyledonous and monocotyledonous forms. On the basis of transcriptome data, the expression patterns of Di19s in poplar under abiotic stress and ABA treatment were further studied. Subsequently, homologous genes PtDi19-2 and PtDi19-7 with strong response to drought stress were identified. PtDi19-2 functions as a nuclear transcriptional activator with a transactivation domain at the C-terminus. PtDi19-7 is a nuclear and membrane localization protein. Additionally, PtDi19-2 and PtDi19-7 were able to interact with each other in yeast two-hybrid system. Overexpression of PtDi19-2 and PtDi19-7 in Arabidopsis was found. Phenotype identification and physiological parameter analysis showed that transgenic Arabidopsis increased ABA sensitivity and drought tolerance. PtDi19-7 was overexpressed in hybrid poplar 84K (Populus alba × Populus glandulosa). Under drought treatment, the phenotype and physiological parameters of transgenic poplar were consistent with those of transgenic Arabidopsis. In addition, exogenous ABA treatment induced lateral bud dormancy of transgenic poplar and stomatal closure of transgenic Arabidopsis. The expression of ABA/drought-related marker genes was upregulated under drought treatment. These results indicated that PtDi19-2 and PtDi19-7 might play a similar role in improving the drought tolerance of transgenic plants through ABA-dependent signaling pathways.

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“…The positive regulatory roles of bHLH transcription factors have been shown in different plants [ 65 ]. Our data analysis revealed higher expression of a gene encoding zinc finger C3H domain-containing protein 53 (cluster-30086.92972) in T03R than T22R ( Figure 5 and Table S13 ), suggesting that this gene might be a contributor to salinity tolerance in guar [ 66 ]. We observed upregulation of a gene (cluster-30086.34639) encoding an MYB transcription factor in T03R compared to C03R in response to salinity ( Figure 5 and Table S13 ).…”

Section: Discussionmentioning

confidence: 99%

Deciphering Molecular Mechanisms Involved in Salinity Tolerance in Guar (Cyamopsis tetragonoloba (L.) Taub.) Using Transcriptome Analyses

Sandhu

Dueñas

Ferreira

et al. 2022

Plants

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Guar is a commercially important legume crop known for guar gum. Guar is tolerant to various abiotic stresses, but the mechanisms involved in its salinity tolerance are not well established. This study aimed to understand molecular mechanisms of salinity tolerance in guar. RNA sequencing (RNA-Seq) was employed to study the leaf and root transcriptomes of salt-tolerant (Matador) and salt-sensitive (PI 340261) guar genotypes under control and salinity. Our analyses identified a total of 296,114 unigenes assembled from 527 million clean reads. Transcriptome analysis revealed that the gene expression differences were more pronounced between salinity treatments than between genotypes. Differentially expressed genes associated with stress-signaling pathways, transporters, chromatin remodeling, microRNA biogenesis, and translational machinery play critical roles in guar salinity tolerance. Genes associated with several transporter families that were differentially expressed during salinity included ABC, MFS, GPH, and P-ATPase. Furthermore, genes encoding transcription factors/regulators belonging to several families, including SNF2, C2H2, bHLH, C3H, and MYB were differentially expressed in response to salinity. This study revealed the importance of various biological pathways during salinity stress and identified several candidate genes that may be used to develop salt-tolerant guar genotypes that might be suitable for cultivation in marginal soils with moderate to high salinity or using degraded water.

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The Roles of CCCH Zinc-Finger Proteins in Plant Abiotic Stress Tolerance

Cited by 73 publications

References 198 publications

CCCH Zinc finger genes in Barley: genome-wide identification, evolution, expression and haplotype analysis

CCCH Zinc finger genes in Barley: genome-wide identification, evolution, expression and haplotype analysis

Homologous Drought-Induced 19 Proteins, PtDi19-2 and PtDi19-7, Enhance Drought Tolerance in Transgenic Plants

Deciphering Molecular Mechanisms Involved in Salinity Tolerance in Guar (Cyamopsis tetragonoloba (L.) Taub.) Using Transcriptome Analyses

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