Nucleo‐cytoplasmic Interactions in the Achievement of Nuclear Synchrony in Dna Synthesis and Mitosis in Multinucleate Cells

Chromosome Pulverization in Chinese Hamster Multinucleate Cells Induced by Bleomycin and Cytochalasin B

“…In contrast, 53% (53/100) of the metaphase cells without PC were labeled, the incidence being equivalent to that expected from the normal G2 length (2.5 hours) of Don cells. The lowered incidence of labeling in the former may be a reflexion of the prolonged G2 period, since a similar phenomenon has been reported in virus-fused cells (Johnson and Rao 1971).…”

“…Johnson and Rao 1971). However, the asynchrony in DNA synthesis and/or mitosis in multinucleate systems is no exception, as reported in several mammalian cell cultures (Sandberg et al 1966;Sandberg 1967, 1968;Burns 1971).…”

“…The present study revealed the last two processes more clearly by the use of two chemicals, BLM and CB, with more precise autoradiographic demonstration of asynchronous DNA synthesis and mitosis in such multinucleate systems. Certain nucleo-cytoplasmic interactions have been suggested indispensable for the achievement of nuclear synchrony in multinucleate cells, and the nuclear asynchrony in large multinucleate cells was accounted for the possible uneven distribution of some cytaplasmic factors (Johnson and Rao 1971). This assumption may be supported by the fact that the higher the number of nuclei involved in the CB-induced multinucleates, the greater the asynchrony was induced.…”

“…Johnson and Rao 1971). It is generally accepted that the phenomenon is induced in interphase nuclei by coexisting, as a result of virus-induced cell fusion, with a set of normal metaphase chromosomes in a common cytoplasm.…”

Mitotic Activity of Polykaryons Fused by Modified PEG-DMSO-Serum Method.

“…Presence of conspicuous peak of multipolar telophase-cytokinesis indicates successful completion of division and formation of mononuclear hybrid cells. Such nuclear synchronization was observed in different kinds of polykaryons, obtained by fusion (Johnson and Harris 1969, Johnson and Rao 1971, Onishchenko and Pronina 1988a.…”