Mitotic Activity of Polykaryons Fused by Modified PEG-DMSO-Serum Method.

Manandhar, Gaurishankar; Khodjakov, Alexey; Онищенко, Г. Е.

doi:10.1508/cytologia.58.377

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…We chose the 1.5 hour incubation period after fusion treatments for ultrastructural study so that the centrioles would be sufficiently exposed to the effect of the new cytoplasmic environment, and in the mean time attainment of synchronization would be minimum. Polykaryons obtained by this method show highest asynchronous mitotic activity at the 1.5 hour period (Manandhar et al, 1993).…”

Section: Cell Fusionmentioning

confidence: 92%

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Centriolar cycle of fused cells

Manandhar

Онищенко

1995

Journal of Cell Science

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Ultrastructure of centrioles of fused cells containing heterophasic interphase nuclei, premature chromosome condensation, and a telophase-like nucleus were studied. The study indicated that the heterophasic cellular environments contributed by the different cell partners exert a mutually opposite effect on the structure of centrioles. The G1-cell partner suppresses replication of S-centrioles. Asynchronous replications of G1 and S-stage centrioles were observed in some G1-S, G1-G2 and S-G2 fused cells. In interphase-mitotic fused cells, centrioles of interphase stages underwent mitotic activation when their nuclei were induced to premature chromosome condensation. Daughter centrioles of G1-, S-, and G2-stages were also capable of mitotic activation independently if they were separated from their mother centriole. Inactive centrioles were observed in some cells containing G1-premature chromosome condensation. When mitotic nuclei were induced to telophase-like nucleus formation, their centrioles were also inactivated. Concomitant events of induced nuclear and centriolar changes suggest that they might have been controlled by the heterophasic cytoplasmic factors through similar pathways.

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Section: Cell Fusionmentioning

confidence: 92%

“…Monolayer cells were fused with a modified method using PEG, DMSO and serum. Assessment of this method is described elsewhere (Manandhar et al, 1993). The cells were rinsed 3-times with 15% DMSO in serum, then treated with 50% PEG (molecular mass 4,000, pH 8) for 1 minute.…”

Section: Cell Fusionmentioning

confidence: 99%