Centriolar cycle of fused cells

Abstract

Ultrastructure of centrioles of fused cells containing heterophasic interphase nuclei, premature chromosome condensation, and a telophase-like nucleus were studied. The study indicated that the heterophasic cellular environments contributed by the different cell partners exert a mutually opposite effect on the structure of centrioles. The G1-cell partner suppresses replication of S-centrioles. Asynchronous replications of G1 and S-stage centrioles were observed in some G1-S, G1-G2 and S-G2 fused cells. In interphase-mitotic fused cells, centrioles of interphase stages underwent mitotic activation when their nuclei were induced to premature chromosome condensation. Daughter centrioles of G1-, S-, and G2-stages were also capable of mitotic activation independently if they were separated from their mother centriole. Inactive centrioles were observed in some cells containing G1-premature chromosome condensation. When mitotic nuclei were induced to telophase-like nucleus formation, their centrioles were also inactivated. Concomitant events of induced nuclear and centriolar changes suggest that they might have been controlled by the heterophasic cytoplasmic factors through similar pathways.

The Development of Methods for the Production of New Molecular Vaccines and Appropriate RNA Fragments to Counteract Unwanted Genes: A Pilot Study

“…According to the scientific literature, a lot of properties of many membrane molecules influence DMSO [ 50 , 51 , 52 ] and of other organic detergents [ 53 , 54 ], as do drastic temperature changes [ 55 ], which could be changed. Many inter-molecular interactions have also proven to be influenced, which has also been proposed as a factor, activating the fusion processes [ 56 ]. In this way, the possibility for transfer of nucleotide (DNA and/or RNA) fragments from a virus to a cellular genome, as well as in the opposite direction (from cellular to viral genome) in these conditions could be proposed, and new methods for the production of novel safe recombinant DNA constructs, molecular vaccines (DNA, RNA and/or protein), as well as for appropriate siRNAs on the basis of adeno-associated virus/parvovirus and poxvirus genomes by appropriate application and processing, could be suggested [ 25 , 26 , 27 ].…”