Chromosome Pulverization in Chinese Hamster Multinucleate Cells Induced by Bleomycin and Cytochalasin B

Ikeuchi, Tatsuro

doi:10.1266/jjg.48.391

Cited by 18 publications

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“…The phenomenon of PCC has been extensivelly studied in virally and/or chemi cally-induced multinucleate cells "in vitro" (Johnston et al 1970, Ikeuchi 1973 and "in vivo" (Kurten and Obe 1975, Yamamoto et al 1986). Most of these studies were only restricted to mammalian cells.…”

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"In vivo" Occurrence of Premature Chromosomes Condensation in Kidncy Cells of Eigenmannia virescens (Pisces, Sternopygidae).

1992

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The technique of premature chromosome condensation (PCC) allows the visualization of chromosomes in G1, S and G2 interphase nuclei when such cells are fused with a mitotic metaphase cell. Johnston and Rao (1970) demonstrated that the fusion of a mitotic metaphase with an interphase cell resulted in the breakdown of the nuclear membrane and the subsequent premature condensation of the interphase chromatin.PCC has been used to study the chro mosome constitution of nondividing cells and in this way, to visualize insults on genetic material induced by Xray-radiation of mammalian spermatids that otherwise do not divide (Drwinga et al. 1979). The phenomenon of PCC has been extensivelly studied in virally and/or chemi cally-induced multinucleate cells "in vitro" (Johnston et al. 1970, Ikeuchi 1973 and "in vivo" (Kurten and Obe 1975, Yamamoto et al. 1986). Most of these studies were only restricted to mammalian cells. Spontaneous cell fusions were first described between tumoral cells (Barski et al. 1961, Atkin 1979 and also in cultured lynphocytes of two sibs with Bloom's syndrome (Otto and Therman 1982), which is the first description of spontaneous fusion between non malignant somatic human cells.The present communication reports recent observations on the spontaneous occurrence of PCC in metaphase spreads from the kidney cells of two out of five specimens of a fish, Eigenmannia virescens (2n=38) manteined for fifteen days before sacrifice in aquaria which was verified to be strongly infested by a ciliate protozoan, Ichthyophthirius multifiliis. Material and methodsThe chromosomal analyses were made on cytological preparations from five specimens of a fish, Eigenmannia virescens belonging to the family Sternopygidae, collected in the Mogi Guacu river, at Pirassununga (SP), Brasil. The specimens were maintained for fifteen days in well aerated aquaria in the laboratory till sacrifice for chromosome preparations.By that time two specimens presented a very strong infestation of a ciliate protozoan, Ichthyophthirius multifiliis, distributed over fish bodies like white velvet spots.Chromosome preparations were made from kidney cells according to the procedure de scribed by Foresti et al. (1981). In order to obtain appropriate chromosome condensation for karyotypic analysis, the concentration of colchicine solution was reduced to 0.05% (1ml/ 100g body weight) and the animals were killed 50 to 60 min after intraperitonealy drug injection.

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confidence: 99%