The Human Leukocyte Test System

Obe, Günter; Beek, Bernd

doi:10.1007/978-1-4615-6625-0_8

Cited by 49 publications

(4 citation statements)

References 134 publications

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“…In the present investigation, it is observed that, at 72 h RT in EAC cells, that is, cancerous cell, there is reduction in chromatid aberrations and it can also be noted that the lowest aberrations were seen at 72 h than at 24 and 48 h RT (Tables 1–3). This agrees with earlier reports where it has been amply proven that the decrease in aberration frequency with increasing culturing time reflects a mechanism of mitotic selection of aberration bearing cells [67]. When the data of normal and cancer cells are taken together with respect to the adaptive response and different recovery times, there is not much difference.…”

Section: Resultssupporting

confidence: 91%

Inducible Protective Processes in Animal Systems XIII: Comparative Analysis of Induction of Adaptive Response by EMS and MMS in Ehrlich Ascites Carcinoma Cells

Mahadimane

Vasudev

2014

Scientifica

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In order to investigate the presence of adaptive response in cancerous cells, two monofunctional alkylating agents, namely, ethyl methanesulfonate (EMS) and methyl methanesulfonate (MMS), were employed to treat Ehrlich ascites carcinoma (EAC) cells in vivo. Conditioning dose of 80 mg/kg body weight of EMS or 50 mg/kg body weight of MMS and challenging dose of 240 mg/kg body weight of EMS or 150 mg/kg body weight of MMS were selected by pilot toxicity studies. Conditioned EAC cells when challenged after 8 h time lag resulted in significant reduction in chromosomal aberrations compared to challenging dose of respective agents. As has been proved in earlier studies with normal organisms, even in cancerous cells (EAC), there is presence of adaptive response to methylating and ethylating agents. Furthermore, it is also interesting to note in the present studies that the methylating agent, MMS, is a stronger inducer of the adaptive response than the ethylating agent, EMS.

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Section: Resultssupporting

confidence: 91%

Inducible Protective Processes in Animal Systems XIII: Comparative Analysis of Induction of Adaptive Response by EMS and MMS in Ehrlich Ascites Carcinoma Cells

Mahadimane

Vasudev

2014

Scientifica

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“…[40–41] The decrease in DNA damage in followed up cases could be attributed to three independent physiological processes: repair of DNA damage, elimination of cells due to death of highly damaged cells, dilution of cells carrying DNA damage by the production of undamaged lymphocytes from the stem cell pool. [42–44] Earlier followed up studies have revealed that comet assay is more efficient in detecting ongoing exposure rather than accumulated exposure. [234546] Non-exposed farmers were not compared with the follow up cases as longitudinal study on the same subjects is a powerful mean to observe modification than a comparison between two different populations.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of DNA damage in agricultural workers exposed to pesticides using single cell gel electrophoresis (comet) assay

Kaur¹,

Kaur²,

Lata³

2011

Indian J Hum Genet

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BACKGROUND:Pesticides are used in agriculture to protect crops, but they pose a potential risk to farmers and environment. The aim of the present study is to investigate the relation between the occupational exposure to various pesticides and the presence of DNA damage.MATERIALS AND METHODS:Blood samples of 210 exposed workers (after a day of intense spraying) and 50 control subjects belonging to various districts of Punjab (India) were evaluated using Comet assay. Sixty workers who showed DNA damage were selected for follow up at 5-6 months after the first sampling during a low or null spraying period.RESULTS:Significant differences were found in DNA damage between freshly exposed workers and controls and freshly exposed and followed up cases. There was significant increase in the comet parameters viz. mean comet tail length and frequency of cells showing migration in exposed workers as compared to controls (72.22 ± 20.76 vs. 46.92 ± 8.17, P<0.001; 31.79 vs. 5.77, P<0.001). In the second samples, followed up cases showed significant decrease in frequency of damaged cells as compared to freshly exposed workers of first sampling (P<0.05). The confounding factors such as variable duration of pesticide exposure, age, smoking, drinking and dietary habits etc which were expected to modulate the damage, were instead found to have no significant effect on DNA fragmentation.CONCLUSION:The evidence of a genetic hazard related to exposure resulting from the intensive use of pesticides stresses the need for educational programs for agricultural workers to reduce the use of chemicals in agriculture.

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“…Triaziquone (Oncovedex ® ) was used clinically in the 1960s for the treatment of a number of cancers, but because of its toxicity to bone marrow and blood vessel walls, it has been replaced by more effective agents. 29 Apaziquone (EO9), which contains only one azidirine moiety, is a bioreductive drug that showed no activity in phase II clinical trials when administered intravenously because of rapid pharmacokinetic elimination and poor penetration through vascular tissue. However, later studies showed that it is active and well tolerated in patients with superficial transitional cell carcinoma of the bladder after intravesical administration, and it has been granted "fast track" status by the FDA for this indication.…”

Section: Aziridines (Ethyleneimines)mentioning

confidence: 99%

DNA Alkylating Agents

Avendaño

2015

Medicinal Chemistry of Anticancer Drugs

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The Human Leukocyte Test System

Cited by 49 publications

References 134 publications

Inducible Protective Processes in Animal Systems XIII: Comparative Analysis of Induction of Adaptive Response by EMS and MMS in Ehrlich Ascites Carcinoma Cells

Inducible Protective Processes in Animal Systems XIII: Comparative Analysis of Induction of Adaptive Response by EMS and MMS in Ehrlich Ascites Carcinoma Cells

Evaluation of DNA damage in agricultural workers exposed to pesticides using single cell gel electrophoresis (comet) assay

DNA Alkylating Agents

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