Nucleation and characterization of hydroxyapatite on thioglycolic acid-capped reduced graphene oxide/silver nanoparticles in simplified simulated body fluid

Zhao, Jun; Zhang, Zhaochun; Yu, Zhenwei; He, Zhenni; Yang, Shanshan; Jiang, Huiyi

doi:10.1016/j.apsusc.2013.10.106

Cited by 22 publications

(14 citation statements)

References 43 publications

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“…This is attributed to the ability of these functional groups to attract Ca 2þ and PO 4 32 ions, thus increasing the degree of local supersaturation with respect to calcium phosphate precipitation at regions close to the surfaces [50,53]. While there have been many studies investigating the inhibitory effect of AAs dissolved in solution on HA mineralization [6,[18][19][20][21][22][23][24][25][26][27][28], the effect of AAs bound to surfaces has been subject of just a few studies [8,55,56]. Rautaray et al [8] investigated HA precipitation in the presence of Aspcapped gold nanoparticles (figure 4).…”

Section: Amino Acids Bound To Surfacesmentioning

confidence: 99%

The role of amino acids in hydroxyapatite mineralization

Tavafoghi¹,

Cerruti²

2016

J. R. Soc. Interface.

197

147

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Polar and charged amino acids (AAs) are heavily expressed in noncollagenous proteins (NCPs), and are involved in hydroxyapatite (HA) mineralization in bone. Here, we review what is known on the effect of single AAs on HA precipitation. Negatively charged AAs, such as aspartic acid, glutamic acid (Glu) and phosphoserine are largely expressed in NCPs and play a critical role in controlling HA nucleation and growth. Positively charged ones such as arginine (Arg) or lysine (Lys) are heavily involved in HA nucleation within extracellular matrix proteins such as collagen. Glu, Arg and Lys intake can also increase bone mineral density by stimulating growth hormone production. In vitro studies suggest that the role of AAs in controlling HA precipitation is affected by their mobility. While dissolved AAs are able to inhibit HA precipitation and growth by chelating Ca 2þ and PO 4 32 ions or binding to nuclei of calcium phosphate and preventing their further growth, AAs bound to surfaces can promote HA precipitation by attracting Ca 2þ and PO 4 32 ions and increasing the local supersaturation. Overall, the effect of AAs on HA precipitation is worth being investigated more, especially under conditions closer to the physiological ones, where the presence of other factors such as collagen, mineralization inhibitors, and cells heavily influences HA precipitation. A deeper understanding of the role of AAs in HA mineralization will increase our fundamental knowledge related to bone formation, and could lead to new therapies to improve bone regeneration in damaged tissues or cure pathological diseases caused by excessive mineralization in tissues such as cartilage, blood vessels and cardiac valves.

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Section: Amino Acids Bound To Surfacesmentioning

confidence: 99%

The role of amino acids in hydroxyapatite mineralization

Tavafoghi¹,

Cerruti²

2016

J. R. Soc. Interface.

197

147

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“…In general, GFNs are good HA nucleators due to their high surface area, and the presence of biomolecules always increase the amount and rate of HA precipitation [20,21,38,40,42]. Liu et al [20,38] showed the formation of HA on reduced GO (rGO) modified with a layer of in situ polymerized dopamine ( polydopamine, PDA) after one week and gelatinmodified GO after two weeks of immersion in 1.5 Â SBF.…”

Section: Arg-carboxylated Graphene Oxide As a Strong Nucleator Of Hydmentioning

confidence: 99%

“…A few papers have started exploring the formation of HA on these materials [36 -42]. Also, a few researchers have explored the modification of GFNs with biomolecules and investigated the effect of such modifications on in vitro HA mineralization [20,21,36,38,40,42]. All these studies showed that HA precipitation was improved in the presence of biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite formation on graphene oxide modified with amino acids: arginine versus glutamic acid

Tavafoghi¹,

Brodusch²,

Gauvin³

et al. 2016

J. R. Soc. Interface.

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Hydroxyapatite (HA, Ca 5 (PO 4 ) 3 OH) is the main inorganic component of hard tissues, such as bone and dentine. HA nucleation involves a set of negatively charged phosphorylated proteins known as non-collagenous proteins (NCPs). These proteins attract Ca 2þ and PO 4 32 ions and increase the local supersaturation to a level required for HA precipitation. Polar and charged amino acids (AAs) are highly expressed in NCPs, and seem to be responsible for the mineralizing effect of NCPs; however, the individual effect of these AAs on HA mineralization is still unclear. In this work, we investigate the effect of a negatively charged (Glu) and positively charged (Arg) AA bound to carboxylated graphene oxide (CGO) on HA mineralization in simulated body fluids (SBF).Our results show that Arg induces HA precipitation faster and in larger amounts than Glu. We attribute this to the higher stability of the complexes formed between Arg and Ca 2þ and PO 4 32 ions, and also to the fact that Arg exposes both carboxyl and amino groups on the surface. These can electrostatically attract both Ca 2þ and PO 4 32 ions, thus increasing local supersaturation more than Glu, which exposes carboxyl groups only.

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“…Biomineralization is an extremely complex process, in which, the initial nucleation has strong impact on mineral growth, leading to diversities in Ca/P ratio, morphology, crystal size and structure of final CaP compounds [11][12][13]. Amino acids were reported able to take part in the initial nucleation during the formation of amorphous calcium carbonate phase, thus causing changes in the subsequent crystal growth orientation and lattice parameters of the final product [14][15][16][17].…”

Section: Introductionmentioning

confidence: 98%