Mineralization on polylactide/gelatin composite nanofibers using simulated body fluid containing amino acid

Guo, Yougang; Lan, Jinle; Zhang, Caijin; Cao, Man; Cai, Qing; Yang, Xiaoping

doi:10.1016/j.apsusc.2015.05.047

Cited by 26 publications

(13 citation statements)

References 51 publications

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“…The presence of 50 wt % of hydrophobic PLLA component in PG‐B0 had prevented the swelling of gelatin component and the conglutination between fibers . Conversely, PG‐B0 was quite hydrophilic due to the presence of 50 wt % of gelatin component, which made the nanofibers able to be soaked immediately when they were immersed into SBF . To readily control the amount and the crystalline structure of minerals depositing onto nanofibers via timed biomineralization, a modified 5 × SBF with continuous CO 2 bubbling was applied.…”

Section: Discussionmentioning

confidence: 99%

“…Biomineralization is a complex process, especially when differently‐designed SBFs were used . Because of the variances in both the SBFs and the soaking times, generally, the resulted mineral depositions are different in many aspects such as chemical composition, morphology, and amount . In most of the publications, the major concern was the ability of biomineralized substrates in enhancing osteogenic differentiation in comparison with the pristine materials.…”

Section: Introductionmentioning

confidence: 99%

“…To this end, composite nanofibers composed of poly( l ‐lactide) (PLLA) and gelatin in 1:1 weight ratio were prepared via electrospinning and submitted to biomineralization study. PLLA/gelatin composite nanofibers have been identified able to remain fiber morphology during crosslinking treatment and provide nucleation sites for mineral depositions from our previous studies . Five times SBF (5 × SBF) was used for inducing fast mineral deposition to avoid the possible degradation of PLLA/gelatin composite nanofibers during biomineralization.…”

Section: Introductionmentioning

confidence: 99%

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Regulating proliferation and differentiation of osteoblasts on poly(l-lactide)/gelatin composite nanofibers via timed biomineralization

Zhang

Cao

Lan

et al. 2016

J. Biomed. Mater. Res.

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Mimicking the natural bone extracellular matrix, biomineralized nanofibers are envisioned as good choices for bone regeneration. Herein, composite nanofibers composed of poly(L-lactide) (PLLA) and gelatin (50/50, w/w) were electrospun and soaked in a modified five times simulated body fluid (SBF) for 6-24 h. Along with the soaking time, the amounts of deposited minerals increased, and the minerals transformed from dicalcium phosphate dehydrate (DCPD) to hydroxyapatite (HA). Mineral dissolution and Ca(2+) ion release of these biomineralized nanofibers were investigated by putting them in deionized water or Hank's balanced salt solution. MC3T3-E1 osteoblasts were cultured in transwell chambers without contacting the materials or on the biomineralized nanofibers directly. In the noncontact culture, the released ions were found able to enhance osteogenic differentiation more significantly in comparison with cell proliferation. In the contact culture, all biomineralized nanofibers demonstrated strong ability in promoting both cell proliferation and osteogenic differentiation. Due to the fast dissolution of DCPD, the biomineralized nanofibers obtained from short SBF soaking was found to be inferior in enhancing osteogenic differentiation. Whereas the cells displayed high levels of alkaline phosphatase activity and collagen I synthesis when the material had abundant deposition of apatite. The results revealed that cell biological behaviors were synergistically influenced by the ionic dissolution products, the composition, and the morphology of biomineralized nanofibers, which could be regulated by timed biomineralization. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1968-1980, 2016.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regulating proliferation and differentiation of osteoblasts on poly(l-lactide)/gelatin composite nanofibers via timed biomineralization

Zhang

Cao

Lan

et al. 2016

J. Biomed. Mater. Res.

Self Cite

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“…6 Guo et al biomineralized PLLA/gelatin nanobers in an amino-acid-rich articial body uid and found that the presence of amino acids increased the preferential growth of HA crystals along the c axis, promoting the formation of HA acicular crystals. 7 However, in vitro bio-mineralization in simulated body uid (SBF) needs at least several weeks for the desirable outcomes, and long immersion times can lead to drug release and material degradation. [8][9][10] Therefore, an efficient deposition method for calcied HA bioactive coatings on brous scaffold surfaces remains a challenge and an urgent issue that needs to be solved.…”

Section: Introductionmentioning

confidence: 99%

Accelerated fabrication of antibacterial and osteoinductive electrospun fibrous scaffolds via electrochemical deposition

Wang

Gao

et al. 2018

RSC Adv.

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Electrospun fibrous scaffolds have attracted much research interest due to their many applications in orthopedics and other relevant fields. However, poor surface bioactivity of the polymer scaffold body significantly limits the implementation of many potential applications, and an effective solution remains a great challenge for researchers. Herein, a highly efficient method, namely pulsed electrochemical deposition (ED) with co-electrospinning nano-Ag dopant, to fabricate poly(L-lactic acid) (PLLA)/nano-Ag composite fibers is presented. The resulting product demonstrated excellent antibacterial properties, as well as strong capabilities in facilitating the precipitation of calcium phosphate crystals at fiber surfaces and in promoting osteogenic differentiation. In the process of ED, the conductivity of the fibers was observed to increase due to the nano-Ag dopant. Upon applying pulse signals when charging, water electrolysis occurred in micro-reactive regions of anodic fibers, forming OH À , an alkaline environment that allowed the supersaturation of calcium phosphate. When discharging, the calcium phosphate in the solution diffused rapidly and reduced the concentration polarization, reforming a homogeneous electrolyte. The realization of efficient bioactive coatings at fiber surfaces was achieved in a highly efficient manner by repeating the above charging and discharging processes. Therefore, ED can be adopted to simplify and accelerate the fabrication process of an osteogenetic and antibacterial electrospun fibrous scaffold.

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“…The most commonly explored porous bone scaffold materials include bioceramics and bioactive glass (Chen et al 2013;Chen et al 2014) and polymers (Owen et al 2016). Plus there are many kinds of biocompatible materials have been used to guide mineralization of HA to make them suitable as bone repair materials, including ceramics (Dai et al 2015), metallics , polymeric materials (Huang et al 2015), and their composites (Guo et al 2015), cellulose (Li et al 2012;Morimune-Moriya et al 2015), and collagen (Tomoaia and Pasca 2015).…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Growth of Hydroxyapatite on Kenaf Fibers

et al. 2016

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Mineralization on polylactide/gelatin composite nanofibers using simulated body fluid containing amino acid

Cited by 26 publications

References 51 publications

Regulating proliferation and differentiation of osteoblasts on poly(l-lactide)/gelatin composite nanofibers via timed biomineralization

Regulating proliferation and differentiation of osteoblasts on poly(l-lactide)/gelatin composite nanofibers via timed biomineralization

Accelerated fabrication of antibacterial and osteoinductive electrospun fibrous scaffolds via electrochemical deposition

Biomimetic Growth of Hydroxyapatite on Kenaf Fibers

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