Hydroxyapatite formation on graphene oxide modified with amino acids: arginine versus glutamic acid

Tavafoghi, Maryam; Brodusch, Nicolas; Gauvin, Robert; Cerruti, Marta

doi:10.1098/rsif.2015.0986

Cited by 44 publications

(26 citation statements)

References 71 publications

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“…In that case, CaP nucleation probably occurred on the surface of glass particles present on the GS surface of composites in accordance with mechanism of bioactivity proposed by Kokubo and Hench [36]. It was previously shown that the layers of proteins and amino acids on the surfaces of graphene oxide and polymers (PS, PMMA) induced the nucleation of hydroxyapatite in SBF and 1.5SBF [37,38], which can explain the enrichment of pure PCL film surface with Ca and P ( Fig. 1) with an accompanying decrease in Ca and P concentration in DMEM-10% FBS (Fig.…”

Section: In Vitro Bioactivitysupporting

confidence: 54%

A new insight into in vitro behaviour of poly(ε-caprolactone)/bioactive glass composites in biologically related fluids

et al. 2017

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In the present work, the role of content, size and chemical composition of gel-derived bioactive glass particles from the SiO 2 -CaO-P 2 O 5 system in modulating the in vitro bioactivity, osteoinductive properties and long-term (up to 15 months) degradation behaviour of poly(e-caprolactone)-based composite films was investigated. Bioactivity was assessed in simulated body fluid (SBF) and HEPES-free Dulbecco's modified Eagle medium (DMEM) supplemented with 10% foetal bovine serum (FBS), while hydrolytic degradation tests were performed in phosphate buffer saline. Obtained composite films showed excellent calcium phosphate (CaP) layer forming ability in both SBF and DMEM-10% FBS. However, kinetics of bioactivity process strongly depended on the type of medium used. The layer of amino acids and proteins, derived from cell culture medium, on the surfaces of composites created barrier that inhibited release of the ions on the one hand, while increasing nucleation density of calcium phosphates, affecting the morphology of formed CaP layers on the other. The presence of bioactive glass fillers was shown to impart osteoinductive properties to obtained films, supporting osteoblast attachment and proliferation, as well as stimulating cell differentiation and also matrix mineralization process in vitro. We showed that kinetics of bioactivity process and also osteoinductive properties of composite films could be easily modulated with the use of different contents and chemical compositions of fillers. The results showed that modification of PCL matrix with bioactive glass particles accelerated its degradation. We proved that the degradation rate of composites could be controlled and optimized for bone regeneration, in particular by using bioactive fillers causing different calcium phosphate layer forming ability on the surfaces of composites, depending on particle size and chemical composition. We have presented new opportunities to design and obtain multifunctional composites with tunable degradation and bioactivity kinetics, as well as biological properties that can meet complex requirements of bone tissue engineering.

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Section: In Vitro Bioactivitysupporting

confidence: 54%

A new insight into in vitro behaviour of poly(ε-caprolactone)/bioactive glass composites in biologically related fluids

et al. 2017

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“…The ability of a substrate to self‐mineralize, via selected adsorption of elements from the surrounding environment is a significantly beneficial material property in the development of biomimetic surfaces. The role of surface modification, specifically –NH 2 and –COOH modified layers, in enhancing the ability of a material to produce an apatite‐like layer when submerged in stimulated body fluids has previously been reported . Yet, the role of varying the chain length with a view to optimizing this process has not previously been reported.…”

Section: Discussionmentioning

confidence: 99%

The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells

Chen

Hunt

Fawcett

et al. 2018

J Biomedical Materials Res

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Silane modification has been proposed as a powerful biomaterial surface modification tool. This is the first comprehensive investigation into the effect of silane chain length on the resultant properties of -NH silane monolayers and the associated osteoinductive properties of the surface. A range of -NH presenting silanes, chain length 3-11, were introduced to glass coverslips and characterized using water contact angles, atomic force microscopy, X-ray photoelectron spectroscopy, and Ninhydrin assays. The ability of the variation in chain length to form a homogenous layer across the entirety of the surfaces was also assessed. The osteoinductive potential of the resultant surfaces was evaluated by real-time polymerase chain reaction, immunocytochemistry, and von Kossa staining. Control of surface chemistry and topography was directly associated with changes in chain length. This resulted in the identification of a specific, chain length 11 (CL11) which significantly increased the osteoinductive properties of the modified materials. Only CL11 surfaces had a highly regular nano-topography/roughness which resulted in the formation of an appetite-like layer on the surface that induced a significantly enhanced osteoinductive response (increased expression of osteocalcin, CBFA1, sclerostin, and the production of a calcified matrix) across the entirety of the surface. © 2018 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1862-1877, 2018.

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“…The common functional methods of GDs used in bone repair were carboxylation, 103 – 105 silanization, 106 , 107 hydroxylation, 25 dopamine modification 108 , 109 and amination, 24 , 110 as shown in Table 1 . These functional modifications improved the different properties of the composites, such as binding other ions, dispersibility, hydrophilicity, adsorption of serum proteins and attraction to osteoblasts.…”

Section: Utilization Of Advantages Of Gds In Bone Repair Materialsmentioning

confidence: 99%

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Du¹,

Wang

Zhang³

et al. 2020

IJN

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During continuous innovation in the preparation, characterization and application of various bone repair materials for several decades, nanomaterials have exhibited many unique advantages. As a kind of representative two-dimensional nanomaterials, graphene and its derivatives (GDs) such as graphene oxide and reduced graphene oxide have shown promising potential for the application in bone repair based on their excellent mechanical properties, electrical conductivity, large specific surface area (SSA) and atomic structure stability. Herein, we reviewed the updated application of them in bone repair in order to present, as comprehensively, as possible, their specific advantages, challenges and current solutions. Firstly, how their advantages have been utilized in bone repair materials with improved bone formation ability was discussed. Especially, the effects of further functionalization or modification were emphasized. Then, the signaling pathways involved in GDs-induced osteogenic differentiation of stem cells and immunomodulatory mechanism of GDs-induced bone regeneration were discussed. On the other hand, their applications as contrast agents in the field of bone repair were summarized. In addition, we also reviewed the progress and related principles of the effects of GDs parameters on cytotoxicity and residues. At last, the future research was prospected.

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Hydroxyapatite formation on graphene oxide modified with amino acids: arginine versus glutamic acid

Cited by 44 publications

References 71 publications

A new insight into in vitro behaviour of poly(ε-caprolactone)/bioactive glass composites in biologically related fluids

A new insight into in vitro behaviour of poly(ε-caprolactone)/bioactive glass composites in biologically related fluids

The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

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