NRP1 regulates HMGB1 in vascular endothelial cells under high homocysteine condition

Ma, Yeshuo; Zhang, Zhen; Chen, Runtai; Shi, Rui; Zeng, Pingyu; Chen, Ruifang; Leng, Yiping; Chen, Alex F.

doi:10.1152/ajpheart.00746.2018

Cited by 15 publications

(16 citation statements)

References 42 publications

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“…And the molecular mechanism of VEGF-Nrp1 interaction is insufficiently reported [40]. Ma et al found that overexpression of Nrp1 promoted the expression and secretion of high mobility group box 1 (HMGB1) and endothelial inflammation, and that the mitogen-activated protein kinase (MAPKs) pathway was involved in this process [41]. Consistent with the above studies, we also found that NRP1 was highly expressed in GBM patients.…”

Section: Discussionsupporting

confidence: 88%

Computational analysis and verification of molecular genetic targets for glioblastoma

Li¹,

Liu²,

Chen³

et al. 2020

Bioscience Reports

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Background: Glioblastoma (GBM) is the most common malignant brain tumor with a poor prognosis. The initial treatment for high-grade gliomas is surgical excision. However, even with concomitant use of radiation or chemotherapy, patients are still prone to recurrence. The specific pathogenesis of GBM is still controversial. Methods: Differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) between GBM and normal brain tissues were screened. P-value was obtained by Bayes test based on the limma package. Statistical significance was set as P-value <0.05 and |Fold change (FC)| > 0.2 (GSE90886); P-value <0.05 and |FC| > 1 (GSE116520, GSE103228). Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG), protein–protein interaction (PPI) network were performed. Hub genes were selected from miRNA target genes and DEGs. GBM and normal brain tissues were extracted to verify the expression. Results: A total of 100 DEGs were overlapped in both datasets. Analysis of pathways and process enrichment tests indicated that ion transport, positive regulation of macromolecule metabolic process, cell cycle, axon guidance were enriched in the GBM. Sixteen hub genes were identified. Hub genes ADARB1 and neuropilin 1 (NRP1) were significantly associated with overall survival (OS) and disease-free survival (DFS) (P<0.05). Eukaryotic translation termination factor 1 (ETF1) was associated with DFS (P<0.05). Conclusions: DEGs and DEMs were found between GBM tumor tissues and normal brain tissues. These biomarkers may be used as targets for early diagnosis and specific treatment.

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Section: Discussionsupporting

confidence: 88%

Computational analysis and verification of molecular genetic targets for glioblastoma

Li¹,

Liu²,

Chen³

et al. 2020

Bioscience Reports

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“…The collected serum was placed in a refrigerator at -80˚C for testing. The level of homocysteine (Hcy) was detected by cyclic enzyme method ( 19 ). The level of miR-28-3p was detected by real-time fluorescence quantitative PCR (qRT-PCR).…”

Section: Methodsmentioning

confidence: 99%

Expression of miR‑28‑3p in patients with Alzheimer's disease before and after treatment and its clinical value

2020

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Expression of miR-28-3p in patients with Alzheimer's disease (AD) before and after treatment and clinical value of miR-28-3p were determined. There were three groups: 68 AD patients treated with donepezil combined with basic therapy in The People's Hospital of Shouguang collected as an AD group, 70 patients with mild cognitive impairment (MCI) as an MCI group, and 75 healthy people as a normal group. Serum miR-28-3p was detected by qRT-PCR. The Montreal cognitive assessment scale (MoCA), mini mental state examination scale (MMSE), activities of daily living scale (ADL) and homocysteine (Hcy) were adopted to assess patients before and after treatment. miR-28-3p in normal group was significantly lower than that in other two groups, and miR-28-3p in MCI group was significantly lower than that in AD group (P<0.001). miR-28-3p correlated with the course and severity of patients. miR-28-3p in AD group after treatment was significantly lower than that before treatment (P<0.001). ADL and Hcy of AD patients after treatment were significantly lower than before treatment (P<0.05), and MMSE and MoCA after treatment were significantly higher than before treatment (P<0.05). Before and after treatment, miR-28-3p was significantly positively correlated with ADL score and Hcy level, but negatively correlated with MMSE score and MoCA score. Analysis of the working characteristic curve of the patients indicated that miR-28-3p can be used for diagnosis of AD patients. Donepezil therapy may reduce miR-28-3p level to alleviate the symptoms of AD patients, and miR-28-3p level can be used as an early diagnosis and prognosis evaluation of AD patients.

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“…NRP1 is also a co-receptor for the VEGF signaling pathway, indicating a crosstalk between these two pathways. NRP1 also regulates HMGB1, which induces caveolae formation in general ECs (Ma et al, 2019). Possibly PDGF signaling is able to manage transcytosis via this pathway, additionally activating a regular PI3K/AKT pathway for actin dynamic regulation.…”

Section: Platelet-derived Growth Factor (Pdgf)-b/pdgf Receptor Beta (Pdgfrβ)mentioning

confidence: 99%

Overview of Crosstalk Between Multiple Factor of Transcytosis in Blood Brain Barrier

et al. 2020

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Blood brain barrier (BBB) conserves unique regulatory system to maintain barrier tightness while allowing adequate transport between neurovascular units. This mechanism possess a challenge for drug delivery, while abnormality may result in pathogenesis. Communication between vascular and neural system is mediated through paracellular and transcellular (transcytosis) pathway. Transcytosis itself showed dependency with various components, focusing on caveolae-mediated. Among several factors, intense communication between endothelial cells, pericytes, and astrocytes is the key for a normal development. Regulatory signaling pathway such as VEGF, Notch, S1P, PDGFβ, Ang/Tie, and TGF-β showed interaction with the transcytosis steps. Recent discoveries showed exploration of various factors which has been proven to interact with one of the process of transcytosis, either endocytosis, endosomal rearrangement, or exocytosis. As well as providing a hypothetical regulatory pathway between each factors, specifically miRNA, mechanical stress, various cytokines, physicochemical, basement membrane and junctions remodeling, and crosstalk between developmental regulatory pathways. Finally, various hypotheses and probable crosstalk between each factors will be expressed, to point out relevant research application (Drug therapy design and BBB-on-a-chip) and unexplored terrain.

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NRP1 regulates HMGB1 in vascular endothelial cells under high homocysteine condition

Cited by 15 publications

References 42 publications

Computational analysis and verification of molecular genetic targets for glioblastoma

Computational analysis and verification of molecular genetic targets for glioblastoma

Expression of miR‑28‑3p in patients with Alzheimer's disease before and after treatment and its clinical value

Overview of Crosstalk Between Multiple Factor of Transcytosis in Blood Brain Barrier

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