Novel wound dressing based on nanofibrous PHBV-keratin mats

Abstract

Keratin is an important protein used for wound healing and tissue recovery. In this study, keratin was first extracted from raw materials and chemically modified to obtain stable keratin (m-keratin). The raw and m-keratin were examined by Raman spectroscopy. The molecular weight of the m-keratin was analysed by SDS-PAGE. The m-keratin was then blended with poly(hydroxybutylate-co-hydroxyvalerate) (PHBV) and electrospun to afford nanofibrous mats. These mats were characterized by field emission scanning electron microscopy (FE-SEM), electron spectroscopy for chemical analysis (ESCA) and atomic force microscopy (AFM). From the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) data, it was found that introduction of keratin enhanced cell proliferation. From wound-healing test and histological examination results, it was shown that the composite mats accelerated wound recovery remarkably as compared to the PHBV control. It was concluded that PHBV-keratin may be a good candidate as a wound dressing.

Evaluation of keratin biomaterial containing silver nanoparticles as a potential wound dressing in full‐thickness skin wound model in diabetic mice

“…In addition, the wound covered with keratin dressing underwent faster epithelization in comparison with the control wounds facilitating epithelial cell migration and proliferation but also favoring a moist environment advisable for wound healing and maintaining mechanical strength important for tissue remodeling. Similar results were obtained by Yuan et al () who examined a wound dressing based on nanofibrous poly(hydroxybutylate‐co‐hydroxyvalerate) (PHBV)–keratin mats in a partial‐thickness skin wound in athymic nude mice. They observed that the wound covered with PHBV–keratin mats underwent epithelization faster with epidermis being thicker when compared with the control wounds.…”

Differences in cytocompatibility between collagen, gelatin and keratin

“…In our previous work, keratins were coelectrospun with PHBV [19,20] and PLA [21], respectively. These mats could accelerate cell adhesion and proliferation.…”

Electrospun PCL/keratin/AuNPs mats with the catalytic generation of nitric oxide for potential of vascular tissue engineering

“…S ‐Carboxymethyl keratins were prepared from human hair in our laboratory . PCL (Mw 80,000) was provided by Daigang Biomaterial Co., Ltd. (Jinan, China).…”