Electrospun PCL/keratin/AuNPs mats with the catalytic generation of nitric oxide for potential of vascular tissue engineering

Wan, Xiuzhen; Liu, Pengcheng; Jin, Xin; Xin, Xuanxuan; Li, Pengfei; Yuan, Jiang; Shen, Jian

doi:10.1002/jbm.a.36521

Cited by 24 publications

(9 citation statements)

References 50 publications

(114 reference statements)

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“…Furthermore, the disulfide bonds endow keratin with redox‐responsiveness (Curcio, Blanco‐Fernandez, Diaz‐Gomez, Concheiro, & Alvarez‐Lorenzo, ) and catalytic activity to generate NO in blood (Jin, Wang, Yuan, & Shen, ). Previously, our group has electrospun keratin composite mats with good cytocompatibility for vascular tissue engineering scaffold (Li, Wang, Ye, Yuan, & Xiao, ; Wan et al, ; Wan et al, ).…”

Section: Introductionmentioning

confidence: 99%

Poly(ε‐caprolactone)/keratin/heparin/VEGF biocomposite mats for vascular tissue engineering

Wan

Jin

et al. 2019

J Biomedical Materials Res

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Vascular endothelial growth factor (VEGF) is an effective growth and angiogenic cytokine, which stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Binding VEGF with heparin could protect it from rapid degradation, subsequently allowing it to be controlled release. Primarily, poly(ε-caprolactone) (PCL) and keratin were coelectrospun, followed by conjugating with heparin and subsequently binding VEGF. The loaded heparin and VEGF on these mats were quantified, respectively. The surface characteristics of mats were investigated by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). The VEGF delivery results indicated these mats could sustainably release VEGF for 2 weeks. Cell viability assays suggested these mats were valid to accelerate human umbilical vein endothelial cells (HUVECs) proliferation, while inhibit human umbilical arterial smooth muscle cells (HUASMCs) growth under the combined actions of VEGF and heparin. The results tested by blood clotting times (APTT, PT, and TT), hemolysis, and platelet adhesion indicated the mats were blood compatible. To sum up, these biocomposite mats are ideal scaffolds for vascular tissue engineering. K E Y W O R D S heparin, keratin, vascular tissue engineering, VEGF

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Section: Introductionmentioning

confidence: 99%

Poly(ε‐caprolactone)/keratin/heparin/VEGF biocomposite mats for vascular tissue engineering

Wan

Jin

et al. 2019

J Biomedical Materials Res

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“…The protein eNOS regulates the availability of NO, a lipophilic compound that takes part in several biological mechanism [50]. NO produced by the activity of eNOS modulate, beyond other functions, the degree of constriction experienced by a blood vessel, cell cycle progression, senescence or apoptosis, immune system cells activity and platelet aggregation [51][52][53][54][55].…”

Section: Resultsmentioning

confidence: 99%

Therapeutic Design of Peptide Modulators of the Interaction Between eNOS and p53 in Atherosclerosis

Ds¹,

Lp²,

Am³

et al. 2019

JICOA

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Atherosclerosis is a cardiovascular disease featuring a chronic inflammation due to the accumulation of lipids within the tunica intima of arteries. The development of the disease depends on dynamic changes in the vascular biology. Immune system cells directly influence the pathogenesis of atherosclerosis during the inflammatory process. Currently, atherosclerosis diagnosis is performed by non-invasive or invasive methods depending on the type of arteries that are being investigated. New diagnostic and therapeutic procedures should improve the quality of life of patients. Some of the genes that could be biomarkers of cardiovascular diseases are TP53 and eNOS. The protein p53 is recognized as a tumor suppressor protein that controls DNA repair, cell cycle progression or arrest and apoptosis. These functions that p53 exerts are well known and some other functions are being investigated, such as its role in the cardiovascular system. The eNOS gene regulates the levels of nitric oxide, which is vital for several intracellular biological functions, such as vasodilation, vascular homeostasis, protection of arteries against injuries, cellular growth, signaling pathways and immune response among others. Here, we used an in-silico approach to predict four models of interaction between clinically important proteins (eNOS and p53), to predict the interface of interaction and to rationally design modulating peptides to be tested in vitro and in vivo and possibly used as a therapeutic agent.

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“…Copper Nanoparticle 186 In addition to Cu, there are other metal elements involved in the catalytic release of NO, such as Au. 187,188 Chelate 189 Covalent bonding by polydopamine [190][191][192] Layer-by-layer assembly 193,194 Organic sulfur Covalent bonding thiolcontaining cysteine 195 This design can control the release of NO by adjusting the proportion of each component and the loading sequence.…”

Section: Endogenousmentioning

confidence: 99%

Endothelialization strategy of implant materials surface: The newest research in recent 5 years

Bian

Chen

Weng

et al. 2022

Journal of Applied Biomaterials & Functional Materials

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In recent years, more and more metal or non-metal materials have been used in the treatment of cardiovascular diseases, but the vascular complications after transplantation are still the main factors restricting the clinical application of most grafts, such as acute thrombosis and graft restenosis. Implant materials have been extensively designed and surface optimized by researchers, but it is still too difficult to avoid complications. Natural vascular endodermis has excellent function, anti-coagulant and anti-intimal hyperplasia, and it is also the key to maintaining the homeostasis of normal vascular microenvironment. Therefore, how to promote the adhesion of endothelial cells (ECs) on the surface of cardiovascular materials to achieve endothelialization of the surface is the key to overcoming the complications after implant materialization. At present, the surface endothelialization design of materials based on materials surface science, bioactive molecules, and biological function intervention and feedback has attracted much attention. In this review, we summarize the related research on the surface modification of materials by endothelialization in recent years, and analyze the advantages and challenges of current endothelialization design ideas, explain the relationship between materials, cells, and vascular remodeling in order to find a more ideal endothelialization surface modification strategy for future researchers to meet the requirements of clinical biocompatibility of cardiovascular materials.

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Electrospun PCL/keratin/AuNPs mats with the catalytic generation of nitric oxide for potential of vascular tissue engineering

Cited by 24 publications

References 50 publications

Poly(ε‐caprolactone)/keratin/heparin/VEGF biocomposite mats for vascular tissue engineering

Poly(ε‐caprolactone)/keratin/heparin/VEGF biocomposite mats for vascular tissue engineering

Therapeutic Design of Peptide Modulators of the Interaction Between eNOS and p53 in Atherosclerosis

Endothelialization strategy of implant materials surface: The newest research in recent 5 years

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