Evaluation of keratin biomaterial containing silver nanoparticles as a potential wound dressing in full‐thickness skin wound model in diabetic mice

Konop, Marek; Czuwara, Joanna; Kłodzińska, Ewa; Laskowska, Anna; Sulejczak, Dorota; Damps, Tatsiana; Zielenkiewicz, Urszula; Brzozowska, Iwona; Sureda, A.; Kowalkowski, Tomasz; Schwartz, Robert A.; Rudnicka, Lidia

doi:10.1002/term.2998

Cited by 46 publications

(38 citation statements)

References 41 publications

(61 reference statements)

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“…is was related to the lack of moisture on the surface of the wound caused to generate some damage in the regenerated tissues at the change of gauze bandage. For the sample covered with PU/F nanofibers, better healing (higher wound closure percentage) was observed due to the higher absorption ability of PU/F wound dressing in comparison with gauze bandage that resulted in taking up wound exudates and providing a moist wound healing environment [53]. Also, relatively hindering property of the prepared scaffold from the penetration of bacteria into the wound surface can be another reason for higher efficiency of PU/F compared to gauze bandage.…”

Section: Wound Healing and Histological Examinationmentioning

confidence: 99%

Preparation of Polyurethane/Pluronic F127 Nanofibers Containing Peppermint Extract Loaded Gelatin Nanoparticles for Diabetic Wounds Healing: Characterization, In Vitro, and In Vivo Studies

Almasian

Najafi

Eftekhari

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Diabetic ulcer is regarded as one of the most prevalent chronic diseases. The healing of these ulcers enhances with the use of herbal extracts containing wound dressings with high antibacterial property and creating a nano-sized controlled release system. In this study, new peppermint extract was incorporated in the polyurethane- (PU-) based nanofibers for diabetic wound healing. The peppermint extract was used as an herbal antimicrobial and anti-inflammatory agent. The absorption ability of the wound dressing was enhanced by addition of F127 pluronic into the polymer matrix. The release of the extract was optimized by crosslinking the extract with gelatin nanoparticles (CGN) and their eventual incorporation into the nanofibers. The release of the extract was also controlled through direct addition of the extract into the PU matrix. The results showed that the release of extract from nanofibers was continued during 144 hours. The prepared wound dressing had a maximum absorption of 410.65% and an antibacterial property of 99.9% against Staphylococcus aureus and Escherichia coli bacteria. An in vivo study indicated on significant improving in wound healing after the use of the extract as an effective compound. On day 14, the average healing rate for samples covered by conventional gauze bandage, PU/F127, PU/F/15 (contained extract), and PU/F/15/10 (contained extract and CGN) prepared with different nanoparticle concentrations of 5 and 10 was 47.1 ± 0.2, 56.4 ± 0.4, 65.14 ± 0.2, and 90.55 ± 0.15%, respectively. Histopathological studies indicated that the wound treated with the extract containing nanofibers showed a considerable inflammation reduction at day 14. Additionally, this group showed more resemblance to normal skin with a thin epidermis presence of normal rete ridges and rejuvenation of skin appendages. Neovascularization and collagen deposition were higher in wounds treated with the extract containing nanofibrous wound dressing compared to the other groups.

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Section: Wound Healing and Histological Examinationmentioning

confidence: 99%

Preparation of Polyurethane/Pluronic F127 Nanofibers Containing Peppermint Extract Loaded Gelatin Nanoparticles for Diabetic Wounds Healing: Characterization, In Vitro, and In Vivo Studies

Almasian

Najafi

Eftekhari

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…Although the presence of the inflammatory cells is more preferable during the healing process, the continuing of this process leads to produce scar tissue. In contrast, in control wounds, neutrophils predominated at this time point with lymphocytes (Konop et al, 2020). On the 21st day, epithelialization expanded greater and faster in the Trial 2 groups compared to the control and Trial 1 groups, and had covered the surface of wound, which can be due to participation of mesenchymal cells and their ability in control of inflammation.…”

Section: Discussionmentioning

confidence: 81%

“…The regeneration of epidermis and dermis layers starts with cell migration of wound edges into the wound, and the histological appearance of the wound tissue was completed on Day 21. H&E staining and counting of leukocytes (lymphocytes and neutrophils), new blood vessels and fibroblasts were evaluated at three time point after scaffold implantation and was shown in Figure S2 and S3 and Table S2 ( Konop et al, 2020). These results revealed that…”

mentioning

confidence: 86%

Using 3D‐bioprinting scaffold loaded with adipose‐derived stem cells to burns wound healing

Roshangar

Rad

Kheirjou

et al. 2021

J Tissue Eng Regen Med

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Three dimensional (3D) printing has recently expanded in popularity and has become an effective approach for tissue engineering. Advances in tissue engineering have increased the effectiveness of cell-based therapies. Indeed, the ultimate goal of such treatment is the development of conditions similar to fetal wound regeneration. In this context, technology of 3D printing also allows researchers to more effectively compose multi-material and cell-laden scaffolds with less effort. In this study, we explored a synthetic gel scaffold derived from 3D bioprinter with or without stem cells to accelerate wound healing and skin defects. Adipose-derived stem cells (ADSCs) were isolated and seeded into 3D bioprinter derived-gel scaffold. Morphological and cell adherence properties of 3D scaffold were assessed by hemotoxylin & eosin (H&E) staining and scanning electron microscopy and cell viability was determined by methylthiazolyldiphenyl-tetrazolium bromide assay. In vivo assessment of the scaffold was done using H&E staining in the full-thickness burn rat model. The experimental groups included; (a) untreated (control), (b) 3D bioprinter derived-gel scaffold (Trial 1), and (c) 3D bioprinter derived-gel scaffold loaded with ADSC (Trial 2). Our results represented 3D bioprinter derived-gel scaffold with or without ADSCs accelerated wound contraction and healing compared to control groups. Epithelization was completed until 21 days after operation in scaffold alone. In scaffold with ADSCs group, epithelization was faster and formed a multi-layered epidermis with the onset of cornification. In conclusion, 3D bioprinter derived-gel scaffold with or without ADSCs has the potential to be used as a wound graft material in skin regenerative medicine.

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“…Keratin biomaterials soluble or insoluble are promising candidates in the field of wound dressing materials due to their tissue biocompatibility and ability to support cell growth [ 8 , 20 ]. Moreover, several studies have shown that insoluble keratin enhances tissue recovery and promotes macrophages infiltration during the skin repair process in healthy and diabetic animals.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, keratin contains the binding motif sequences RGD (Arg-Gly-Asp) and LVD (Leu-Asp-Val), which are also found in extracellular matrix proteins such as fibronectin, laminin, collagens, among others [ 5 , 6 ]. The heterogeneous structure of the keratin scaffolds facilitates cell adhesion and allows surface modification with analgesic or antibacterial agents [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Keratin Scaffolds Containing Casomorphin Stimulate Macrophage Infiltration and Accelerate Full-Thickness Cutaneous Wound Healing in Diabetic Mice

et al. 2021

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Impaired wound healing is a major medical challenge, especially in diabetics. Over the centuries, the main goal of tissue engineering and regenerative medicine has been to invent biomaterials that accelerate the wound healing process. In this context, keratin-derived biomaterial is a promising candidate due to its biocompatibility and biodegradability. In this study, we evaluated an insoluble fraction of keratin containing casomorphin as a wound dressing in a full-thickness surgical skin wound model in mice (n = 20) with iatrogenically induced diabetes. Casomorphin, an opioid peptide with analgesic properties, was incorporated into keratin and shown to be slowly released from the dressing. An in vitro study showed that keratin-casomorphin dressing is biocompatible, non-toxic, and supports cell growth. In vivo experiments demonstrated that keratin-casomorphin dressing significantly (p < 0.05) accelerates the whole process of skin wound healing to the its final stage. Wounds covered with keratin-casomorphin dressing underwent reepithelization faster, ending up with a thicker epidermis than control wounds, as confirmed by histopathological and immunohistochemical examinations. This investigated dressing stimulated macrophages infiltration, which favors tissue remodeling and regeneration, unlike in the control wounds in which neutrophils predominated. Additionally, in dressed wounds, the number of microhemorrhages was significantly decreased (p < 0.05) as compared with control wounds. The dressing was naturally incorporated into regenerating tissue during the wound healing process. Applied keratin dressing favored reconstruction of more regular skin structure and assured better cosmetic outcome in terms of scar formation and appearance. Our results have shown that insoluble keratin wound dressing containing casomorphin supports skin wound healing in diabetic mice.

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Evaluation of keratin biomaterial containing silver nanoparticles as a potential wound dressing in full‐thickness skin wound model in diabetic mice

Cited by 46 publications

References 41 publications

Preparation of Polyurethane/Pluronic F127 Nanofibers Containing Peppermint Extract Loaded Gelatin Nanoparticles for Diabetic Wounds Healing: Characterization, In Vitro, and In Vivo Studies

Preparation of Polyurethane/Pluronic F127 Nanofibers Containing Peppermint Extract Loaded Gelatin Nanoparticles for Diabetic Wounds Healing: Characterization, In Vitro, and In Vivo Studies

Using 3D‐bioprinting scaffold loaded with adipose‐derived stem cells to burns wound healing

Keratin Scaffolds Containing Casomorphin Stimulate Macrophage Infiltration and Accelerate Full-Thickness Cutaneous Wound Healing in Diabetic Mice

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