Novel polymorphism of <i>HSP70</i> gene affected caprine physiological vital parameters

Mohalik, Prasanta Kumar; Sahoo, Siddhant Sekhar; Mishra, Chinmoy; Dash, Sanjaya K.; Nayak, Gangadhar

doi:10.1080/10495398.2020.1726364

Cited by 5 publications

(4 citation statements)

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“…Abundant genetic variations in the HSP70 gene sequences have been reported in some livestock animals, such as in cattle [8][9][10][11], goats [12][13][14], and chickens [15]. HSP70 gene polymorphisms are genetically associated with milk production traits in Frieswal crossbred cattle [8], blood biochemical parameters in Holstein cattle [16], and heat thermotolerance (HTC) in Tharparkar cattle [9].…”

Section: Introductionmentioning

confidence: 99%

Genetic polymorphisms of the 5' untranslated regions of the HSP70 gene in Indonesian cattle populations

Prihandini¹,

Primasari²,

Aryogi³

et al. 2022

Vet World

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Background and Aim: Heat shock proteins (HSPs) are a group of proteins that play a significant role in protecting cells against cellular stress. HSP70 is a conserved, sensitive, and abundant gene associated with heat stress's physiological adaptability. The objective of this study was to reveal the polymorphisms of the partial sequences of the HSP70 gene (5' untranslated region [UTR]) in seven cattle populations in Indonesia. Materials and Methods: Polymerase chain reaction products (551 bp) of the HSP70 gene amplified from 102 animals representing seven cattle populations (Bali, Belgian Blue × Peranakan Ongole [PO] cross, Galekan, Jabres, Madura, PO, and Rambon) were sequenced by DNA sequencing method. Results: Fourteen single-nucleotide polymorphisms (SNPs), generally found at a low frequency, were detected. Among these SNPs, only 1117G>A, 1125A>C, and 1204T>C were polymorphic in all the analyzed breeds. A Chi-square test showed that the majority of the loci were in Hardy–Weinberg equilibrium (p>0.05). Varying levels of observed (0.050-0.571) and expected heterozygosity (0.049-0.500) were noted. The polymorphism information content values (0.048-0.375) indicated that the SNPs in the HSP70 gene showed low-to-moderate polymorphism in the studied populations. Thirty-six haplotypes were defined according to the identified SNPs, of which haplotype Hap5 (CGACGAGAGTGTCC) and Hap4 (CGACGAGAGTGCCC) were generally dominant in the studied samples. The phylogenetic tree showed a close relationship between Bali and Rambon cattle and between Galekan and Jabres cattle, while the Belgian Blue × PO crossbred cattle were farther apart. Conclusion: The polymorphisms in the 5' UTR of the HSP70 gene identified in this study should be further investigated in a larger population to unravel the association between the SNPs and thermotolerance in Indonesian local cattle populations.

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Section: Introductionmentioning

confidence: 99%

Genetic polymorphisms of the 5' untranslated regions of the HSP70 gene in Indonesian cattle populations

Prihandini¹,

Primasari²,

Aryogi³

et al. 2022

Vet World

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“…Thus, it is a highly conserved gene (Tripathy et al, 2021), explaining its rapid expression and production during stress (Silver & Noble, 2012). Several studies have reported that polymorphisms may exist in the HSP70 gene (Abhijith et al, 2021;Mohalik et al, 2021;Raza et al, 2021). However, some studies suggest that polymorphisms could be associated with gene performance, whereas other studies claim that HSP70 nucleotide polymorphisms may indicate resistance to stress (Bhat et al, 2016;Habib et al, 2018b;Mohalik et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have reported that polymorphisms may exist in the HSP70 gene (Abhijith et al, 2021;Mohalik et al, 2021;Raza et al, 2021). However, some studies suggest that polymorphisms could be associated with gene performance, whereas other studies claim that HSP70 nucleotide polymorphisms may indicate resistance to stress (Bhat et al, 2016;Habib et al, 2018b;Mohalik et al, 2021). Depending on their type, mutations may affect transcription, translation, and mRNA transportation (Goymer, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…According to Sodhi et al (2013) and based on the genetic variations in Iraqi goats, the polymorphism in the HSP70 gene-coding region impacts goats' ability to tolerate stress (Afsal et al, 2021;Mohalik et al, 2021). Therefore, animals more tolerant to different forms of stress including heat stress can be selected based on variations in the polymorphisms of HSP70 genes as molecular biomarker (Abbas et al, 2020;Kumar et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

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Genetic profiling of HSP70 gene in local Iraqi goats

2022

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Animals display numerous physiological and behavioral responses that reduce the effects of heat stress. Moreover, genetic variance is strongly associated with responses to heat stress, including variants of heat shock proteins (HSPs) that are necessary for thermoregulation and stress resistance. Herein, we performed the molecular profiling of the HSP70 gene, and its polymorphism was demonstrated as a possible factor in the stress tolerance of local Iraqi goats. A number of different mutations were found owing to seven main polymorphisms. Results indicated the occurrence of silent and missense mutations in sequences obtained for Iraqi local goats. Genetic diversity was observed in the HSP70 gene of Iraqi local goats on the basis of phylogenetic-tree analysis as some mutations occurred once whereas others occurred multiple times. The polymorphisms LC616787, LC616788, and LC616791 were combined with the reference gene in the same branch, whereas polymorphisms (LC616785 and LC616786) and (LC616789 and LC616790) met in different branches, respectively. Moreover, all studied proteins had mismatches in their threedimensional structures. Therefore, the presence of specific genetic differences within the HSP70 gene in Iraqi goats can increase the possibility of selecting animals more suitable to various levels of stress.

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Effects of temperature and relative humidity on behavior and physiological indices in goats

Zhou,

He,

Yang

et al. 2023

Small Ruminant Research

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Novel polymorphism of HSP70 gene affected caprine physiological vital parameters

Cited by 5 publications

References 41 publications

Genetic polymorphisms of the 5' untranslated regions of the HSP70 gene in Indonesian cattle populations

Genetic polymorphisms of the 5' untranslated regions of the HSP70 gene in Indonesian cattle populations

Genetic profiling of HSP70 gene in local Iraqi goats

Effects of temperature and relative humidity on behavior and physiological indices in goats

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