Infectious lymphadenitis in small ruminants which caused by broad ranges of microorganismsis worldwide distributed disease, that subsequently causing significant economic losses to animal production industry. Clinical cases of ovine lymphadenopathy in Awasi sheep are described in this research. These cases were recorded separately in the animal's farm of College of Agriculture, University of Basra / Iraq during November 2017. A 2-4year-old Awasi sheep weighing 30-40 kg, presented to the Large Animal Clinic, College of Veterinary Medicine, University of Basra with an enlargement of superficial lymph nodes. Surgical removals of the affected lymph nodes were done and samples were processed to histopathological and bacteriological examinations. The sheep were tentatively diagnosed with Infectious lymphadenitis. The present study revealed that the infectious lymphadenitis in Awasi sheep were caused by Pseudomonas aeruginosa and the infected sheep might provide evidence for disease transmission and source of infection in the farm as well as human.

The heat shock protein 70 (HSP 70) has important roles in protecting cells and keeping them alive when exposed to different stress conditions. The polymorphism of the hsp 70 gene could be linked with the ability of stress tolerance. This study aimed to determine the polymorphism of the hsp 70 gene in Iraqi buffaloes and study bits effects on the resistance to stress. This study was conducted during from November 2018 to February 2019. The number of buffalo females used was 35 at the age of 4-6 years, which belonged to the local farmers from Basra city, Iraq. The DNA was extraction from the blood samples then the polymerase chain reaction (PCR) amplification was performed. The DNA sequences were analyzed by using bioinformatics analysis. The results of the molecular analysis showed that there were two groups of the hsp 70 gene as a compare with the same genes in GenBank due to silent and missense mutations. Based on these findings, it can be concluded that the Iraqi buffaloes have adapted to the surrounding environmental conditions as a result of the action of HSP 70 proliferation. The hsp 70 gene was a useful biomarker of stress tolerance in buffaloes.

The polymorphisms of the hsp70 gene have been associated with diverse resistance of heat stress in hens. The aim of the current study was to explore the genetic variation of the hsp70 gene in Layer hens that bred in Iraq. One hundred-fifty Lohmann breed hens aged 12 months were used in this study. Blood samples were collected during the period from 1st September to 31st December 2018 and examined for detection the polymorphism of hsp70 gene. We have detected four main polymorphisms groups in the coding region of hsp70 gene among these layer hens. A significant association between the silent and the missense mutations with the polymorphisms of hsp70 gene in Layer hens was found. There was a high homology of the hsp70 gene sequences that obtained from our local layer hens with the related sequences obtained from different hottest and coldest areas. In conclusion, this study demonstrates that the different mutations (silent and missense) in the coding region of the hsp70 gene of these local Layer hens predict improve birds’ability to the tolerance of stress conditions, and highlights the need of further investigations.

The objective of this study was to determine the effect of the injection of different levels of AD3E vitamins in improving the productivity and reproductive performance of the Arabi rams in different seasons. This study was conducted in the animal field of the College of Agriculture-University of Basrah, Karmat Ali, for the period from December 2017 to March 2018 (three months winter) and June 2018 until September 2018 (three months in summer). The study used 21 Arabi sheep with 2.5 years old and weights from 55 to 60 kg, the animals were randomly distributed to three equal groups (each group of 7 rams). The control group was injected with a saline solution (distilled water), the second group with 2 ml of vitamin AD3E and the third group with 4 ml of vitamin AD3E, the injection was performed every two weeks. One month after the injection, the blood samples were collected (4 ml) from the jugular vein. Blood tests (RBC, Hb, PCV, and WBC) and blood plasma separation were performed. The chemical components (total protein, albumin, globulin), some enzymes (AST, ALT), and the hormones (testosterone, ICSH, FSH, and cortisol) were estimated. The summer months significantly (P < 0.05) affected the concentration of hormones (testosterone, ICSH, FSH, and cortisol). AST and ALT of Group 3 was significantly higher (P < 0.05) compared to the rest of the months. Total protein concentration, albumin, and globulin during December, August, and January were significantly higher (P < 0.05) over the rest of the months. G3 was also significantly higher (P < 0.05) than G2 and G1 in concentration. The results showed that WBC, PCV, Hb, and RBC of G3 recorded the highest values (P < 0.05) compared with G2 and G1 during summer.

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