Novel Physically Cross-Linked Curcumin-Loaded PVA/Aloe vera Hydrogel Membranes for Acceleration of Topical Wound Healing: In Vitro and In Vivo Experiments
Abstract:This study aims to prepare novel cross-linked antimicrobial membranes composed of PVA-Aloe vera hydrogels using novel physically cross-linked method via transforming PVA to high crystalline structure using propanol. Curcumin was incorporated to improve the membrane biological properties; while gentamycin improved sharply antimicrobial properties. PVA-Aloe vera hydrogel membranes were analyzed by FTIR, SEM, XRD and TGA measurements for characterizing resultant cross-linked membranes. Physicochemical measurement… Show more
“…It is clear that for prepared Cs-g-PAAm/AgNPs, Cs-g-PAAm/AuNPs, and Cs-g-PAAm/Au–Ag-NPs nanocomposites killed 95.8, 78.6, and 91.7% of the cells, respectively. This means that C-g-PAAm/AgNPs nanocomposite hydrogel inhibits cell proliferation the most effectively with IC 50 value of 8.6 ± 1.34 which is considered a very strong cytotoxic biomaterial 47 . In addition, the greatest of IC 50 values were observed with Cs-g-PAAm/AuNPs nanocomposite hydrogel reaching 39.6 µg/mL.…”
In this study, we prepared a pH-responsive nanocomposite hydrogel based on chitosan grafted with acrylamide monomer and gold nanoparticles using gamma irradiation method (Cs-g-PAAm/AuNPs). The nanocomposite was enhanced with a layer coating of silver nanoparticles to improve the controlled release of the anticancer drug fluorouracil while increasing antimicrobial activity and decreasing the cytotoxicity of silver nanoparticles in nanocomposite hydrogel by combining with gold nanoparticles to enhance the ability to kill a high number of liver cancer cells. The structure of the nanocomposite materials was studied using FTIR spectroscopy and XRD patterns, which demonstrated the entrapment of gold and silver nanoparticles within the prepared polymer matrix. Dynamic light scattering data revealed the presence of gold and silver in the nanoscale with the polydispersity indexes in the mid-range values, indicating that distribution systems work best. Swelling experiments at various pH levels revealed that the prepared Cs-g-PAAm/Au–Ag-NPs nanocomposite hydrogels were highly responsive to pH changes. Bimetallic pH-responsive Cs-g-PAAm/Au–Ag-NPs nanocomposites exhibit strong antimicrobial activity. The presence of AuNPs reduced the cytotoxicity of AgNPs while increasing their ability to kill a high number of liver cancer cells.Cs-g-PAAm/Au–Ag-NPs has a high amount of fluorouracil drug loaded at pH 7.4 reaching 95 mg/g with a maximum drug release of 97% within 300 min. Cs-g-PAAm/Au–Ag-NPs have been recommended to use as oral delivery of anticancer drugs because they secure the encapsulated drug in the acidic medium of the stomach and release it in the intestinal pH.
“…It is clear that for prepared Cs-g-PAAm/AgNPs, Cs-g-PAAm/AuNPs, and Cs-g-PAAm/Au–Ag-NPs nanocomposites killed 95.8, 78.6, and 91.7% of the cells, respectively. This means that C-g-PAAm/AgNPs nanocomposite hydrogel inhibits cell proliferation the most effectively with IC 50 value of 8.6 ± 1.34 which is considered a very strong cytotoxic biomaterial 47 . In addition, the greatest of IC 50 values were observed with Cs-g-PAAm/AuNPs nanocomposite hydrogel reaching 39.6 µg/mL.…”
In this study, we prepared a pH-responsive nanocomposite hydrogel based on chitosan grafted with acrylamide monomer and gold nanoparticles using gamma irradiation method (Cs-g-PAAm/AuNPs). The nanocomposite was enhanced with a layer coating of silver nanoparticles to improve the controlled release of the anticancer drug fluorouracil while increasing antimicrobial activity and decreasing the cytotoxicity of silver nanoparticles in nanocomposite hydrogel by combining with gold nanoparticles to enhance the ability to kill a high number of liver cancer cells. The structure of the nanocomposite materials was studied using FTIR spectroscopy and XRD patterns, which demonstrated the entrapment of gold and silver nanoparticles within the prepared polymer matrix. Dynamic light scattering data revealed the presence of gold and silver in the nanoscale with the polydispersity indexes in the mid-range values, indicating that distribution systems work best. Swelling experiments at various pH levels revealed that the prepared Cs-g-PAAm/Au–Ag-NPs nanocomposite hydrogels were highly responsive to pH changes. Bimetallic pH-responsive Cs-g-PAAm/Au–Ag-NPs nanocomposites exhibit strong antimicrobial activity. The presence of AuNPs reduced the cytotoxicity of AgNPs while increasing their ability to kill a high number of liver cancer cells.Cs-g-PAAm/Au–Ag-NPs has a high amount of fluorouracil drug loaded at pH 7.4 reaching 95 mg/g with a maximum drug release of 97% within 300 min. Cs-g-PAAm/Au–Ag-NPs have been recommended to use as oral delivery of anticancer drugs because they secure the encapsulated drug in the acidic medium of the stomach and release it in the intestinal pH.
“…36 The overlapped patterns are found because cross-linked PVA is more crystalline than non-cross-linked and is showing larger peak area. 53 The graph indicated blending between AV, CS, GG, and glutaraldehyde by representing a minimal shift in 2θ values. The presence of more AV contents in ACGG-3 created more compactness to cause more favorable H-bonding and consequently increasing crystallinity.Figure 6.(a) XRD patterns of ACGG2, CAT-3, and CAT-2 membranes (b) ACGG-3, ACPG-3, and ACPG-2 samples.…”
Since ancient times, medicinal plants have been used as traditional medicine to treat a variety of ailments. Aloe vera (AV) gel’s therapeutic potential is one of the most effective approach in the fabrication of functional materials. The current study aimed to prepare the AV and chitosan (CS) membranes using various cross-linkers that were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform-infrared (FT-IR) spectroscopy, thermal gravimetric analysis (TGA), and ultraviolet-visible (UV-Visible) techniques, as well as swelling ratio and antimicrobial studies. SEM analysis revealed that the membrane is porous, with interconnected pores. The inclusion of AV contents in the membrane improved thermal stability and crystallinity. The swelling ratio of the ACPG-3 membrane with a 2:1 CS to AV ratio was 366%. The membranes showed promising antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pasteurella multocida strains. The findings revealed that polymeric CS/AV membranes have effective potential for use in the biomedical field.
“…Albumin-based hydrogels are used mainly in tissue engineering for several detailed applications, such as wound healing, bone repair, vascular endothelium, scaffold materials and so on. Researchers in Romania constructed a composite hydrogel based on BSA, and the hydrogel showed properties that could be better applied for wound healing ( Kenawy et al, 2021 ). The use of an albumin-based hydrogel (containing BMP2) constructed by Zhang et al can significantly promote the production of new bone within 13 weeks, the result showed it was effective in accelerating the bridging of tibial bone defects ( Kossover et al, 2020 ).…”
Section: Two Key Applications Of Albumin-based Hydrogelsmentioning
Albumin is derived from blood plasma and is the most abundant protein in blood plasma, which has good mechanical properties, biocompatibility and degradability, so albumin is an ideal biomaterial for biomedical applications, and drug-carriers based on albumin can better reduce the cytotoxicity of drug. Currently, there are numerous reviews summarizing the research progress on drug-loaded albumin molecules or nanoparticles. In comparison, the study of albumin-based hydrogels is a relatively small area of research, and few articles have systematically summarized the research progress of albumin-based hydrogels, especially for drug delivery and tissue engineering. Thus, this review summarizes the functional features and preparation methods of albumin-based hydrogels, different types of albumin-based hydrogels and their applications in antitumor drugs, tissue regeneration engineering, etc. Also, potential directions for future research on albumin-based hydrogels are discussed.
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