Ag nanoparticles incorporated onto flour (F)/polyvinyl alcohol (PVA) were performed by green synthesis using gamma irradiation to form (F/PVA/Ag) bio‐based nanocomposite hydrogel. The effect of the hydrogel composition and irradiation dose on the gelation percent was investigated. Characterization of (F/PVA) and (F/PVA/Ag) by Fourier transform infrared spectroscopy and thermogravimetric analysis was was investigated. The formation of Ag nanoparticles in (F/PVA/Ag) was confirmed by UV–Vis spectra, X‐ray diffraction, and transmission electron microscopy. The (F/PVA/Ag) nanocomposite exhibited excellent antibacterial activity against the gram‐positive bacteria Staphylococcus aureus (ATCC 29213) and moderate activity against the gram‐negative bacteria Escherichia coli (ATCC 25922). It exhibited excellent antifungal activity against Candida albicans (ATCC 10231). The results proved that this novel developed (F/PVA/Ag) naocomposite has a great potential for use in biomedical applications such as wound dressing hydrogel.
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